H

Finke, J.; Leuner, Hanscarl; Müller, Christian; Blakenburg, W.; Pauleikhoff, B; Walther-Büel, H.; Kind, Hans; Dietrich, H.; Wieser, St.; Adams, Arthur E.; Hole, G; Bräutigam, W.

doi:10.1007/978-3-642-96154-0_8

Cited by 2 publications

(2 citation statements)

References 18 publications

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“…The TCR/CD3-induced signaling in mature -less T cells has been studied in the setting of tumor-infiltrating lymphocytes by proliferation assays and was found decreased (39). Nevertheless, the chain deficiency in the above cells was accompanied by decreased expression of src family members which may offer an explanation to this phenomenon.…”

Section: Discussionmentioning

confidence: 99%

Altered pattern of TCR/CD3-mediated protein-tyrosyl phosphorylation in T cells from patients with systemic lupus erythematosus. Deficient expression of the T cell receptor zeta chain.

Liossis¹,

Ding²,

Dennis³

et al. 1998

J. Clin. Invest.

311

228

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Cellular immunity aberrations in patients with SLE are underscored by the abnormal early Ag receptor-mediated lymphocyte signal transduction pathway. To further characterize the T cell receptor (TCR)/CD3-initiated signaling defects, we studied 22 patients with SLE, 12 patients with other systemic rheumatic diseases, and 14 normal donors. The early (1 min) TCR/CD3-mediated tyrosine phosphorylation of cellular proteins with a molecular size between 36 and 64 kD was increased in 15 of 21 SLE patients, compared to normal or disease control subjects. The deficiency or absence of a band with a molecular size of approximately 16 kD in the immunoblots of SLE patients led us to investigate the expression of the TCRzeta chain. In immunoblots using anti-zeta antibodies we found that 10 of 22 lupus patients tested lacked the expression of TCRzeta, which was always present in control subjects (P < 0.001). Flow cytometric studies using permeabilized cells confirmed the deficiency or absence of the TCRzeta chain in lupus T cells. Using Northern blots we found that for eight patients tested, the TCRzeta mRNA was missing in three, decreased in three, and apparently normal in two patients (P < 0.003), but was always present in control subjects. Reverse transcriptase-PCR verified Northern blot results. We conclude that TCRzeta chain expression is either decreased or absent in the majority of patients with SLE, but not in patients with other systemic rheumatic diseases, regardless of disease activity, treatment status, or clinical manifestations. The previously described increases in TCR-initiated Ca2+ responses and the herein described increases in TCR-induced protein tyrosine phosphorylation and deficient TCRzeta expression may represent intrinsic defects modulating lupus T cell function.

show abstract

Section: Discussionmentioning

confidence: 99%

Altered pattern of TCR/CD3-mediated protein-tyrosyl phosphorylation in T cells from patients with systemic lupus erythematosus. Deficient expression of the T cell receptor zeta chain.

Liossis¹,

Ding²,

Dennis³

et al. 1998

J. Clin. Invest.

311

228

View full text Add to dashboard Cite

show abstract

“…It is interesting to note that tumor-bearing mice with impaired immune function exhibit a selective loss of the ~" subunit, with its apparent replacement by the related Fce-3' subunit (10). A similar loss of ~" is seen in tumor-infiltrating T ceils from patients with malignancies (11,12).…”

Section: Ecognition Of Foreign Antigens By T Cells Is Dependentmentioning

confidence: 92%

Transcriptional regulation of the T cell antigen receptor zeta subunit: identification of a tissue-restricted promoter.

Rellahan

Jensen

Weissman

1994

The Journal of Experimental Medicine

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The cell surface expression of T cell antigen receptors (TCR) is regulated in part by the limiting synthesis of the zeta subunit. Utilizing fragments from the 5' region of the human zeta gene, two discrete regions that promote transcription were characterized. Both of these elements are located within 125 bases of the most 3' site of transcription initiation. The more proximal (3') promoter exhibits activity in lymphoid as well as nonlymphoid cells. In contrast, the more distal (5') promoter element functions in a tissue-restricted fashion. The tissue-specific promoter is localized to a 29-base fragment. The sequence of this region is remarkable for a stretch of 11 consecutive purines that are required for activity. This element constitutes the only known tissue-specific promoter for an invariant TCR subunit. Consistent with the unique role served by the zeta subunit in assembly of the TCR, this study demonstrates that the expression of the zeta gene is regulated in a fashion distinct from other TCR components.

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H

Cited by 2 publications

References 18 publications

Altered pattern of TCR/CD3-mediated protein-tyrosyl phosphorylation in T cells from patients with systemic lupus erythematosus. Deficient expression of the T cell receptor zeta chain.

Altered pattern of TCR/CD3-mediated protein-tyrosyl phosphorylation in T cells from patients with systemic lupus erythematosus. Deficient expression of the T cell receptor zeta chain.

Transcriptional regulation of the T cell antigen receptor zeta subunit: identification of a tissue-restricted promoter.

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