H2O2 homeostasis in wild-type and ethylene-insensitive Never ripe tomato in response to salicylic acid treatment in normal photoperiod and in prolonged darkness

Takács, Zoltan; Poór, Péter; Borbély, Péter; Czékus, Zalán; Szalai, Gabriella; Tari, Irma

doi:10.1016/j.plaphy.2018.02.026

Cited by 25 publications

(32 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This may lead to the formation of ROS and NO in both cell organelles. In intact leaves, however, ROS can be produced by a number of enzymes, such as NADPH oxidase, polyamine oxidases and peroxidases in various cell compartments, which can also be controlled by SA (Poór et al 2017;Takács et al 2018). SA also inhibits ROS-scavenging systems such as the activity of certain antioxidant enzymes (e.g.…”

Section: Discussionmentioning

confidence: 99%

Salicylic acid-induced ROS production by mitochondrial electron transport chain depends on the activity of mitochondrial hexokinases in tomato (Solanum lycopersicum L.)

et al. 2019

Self Cite

View full text Add to dashboard Cite

The growth regulator, salicylic acid (SA) plays an important role in the induction of cell death in plants. Production of reactive oxygen species (ROS) by mitochondrial electron transport chain (mtETC), cytochrome c (cyt c) release from mitochondria and loss of mitochondrial integrity can be observed during cell death execution in plant tissues. The aim of this work was to study the putative role of hexokinases (HXKs) in the initiation of cell death using tomato (Solanum lycopersicum L.) leaves and mitochondria isolated from plants exposed to a sublethal, 0.1 mM and a cell death-inducing, 1 mM concentrations of SA. Both treatments enhanced ROS and nitric oxide (NO) production in the leaves, which contributed to a concentration-dependent loss of membrane integrity. Images prepared by transmission electron microscopy showed swelling and disorganisation of mitochondrial cristae and vacuolization of mitochondria after SA exposure. Using post-embedding immunohistochemistry, cyt c release from mitochondria was also detected after 1 mM SA treatment. Both SA treatments decreased the activity and transcript levels of HXKs in the leaves and the total mtHXK activity in the mitochondrial fraction. The role of mitochondrial hexokinases (mtHXKs) in ROS and NO production of isolated mitochondria was investigated by the addition of HXK substrate, glucose (Glc) and a specific HXK inhibitor, N-acetylglucosamine (NAG) to the mitochondrial suspension. Both SA treatments enhanced ROS production by mtETC in the presence of succinate and ADP, which was slightly inhibited by Glc and increased significantly by NAG in control and in 0.1 mM SA-treated mitochondria. These changes were not significant at 1 mM SA, which caused disorganisation of mitochondrial membranes. Thus the inhibition of mtHXK activity can contribute to the mitochondrial ROS production, but it is not involved in NO generation in SA-treated leaf mitochondria suggesting that SA can promote cell death by suppressing mtHXK transcription and activity.

show abstract

Section: Discussionmentioning

confidence: 99%

Salicylic acid-induced ROS production by mitochondrial electron transport chain depends on the activity of mitochondrial hexokinases in tomato (Solanum lycopersicum L.)

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…Research in the past few years has documented the role of ET in the regulation of heat stress responses in plants (Cheng et al 2013;Takács et al 2018;Pistelli et al 2019;Wu and Yang 2019). ET, being a gaseous plant hormone, exhibit time and dose-dependent effects on the plants during heat stress conditions.…”

Section: Ethylene Biosynthesis and Signaling During Heat Stressmentioning

confidence: 99%

“…ROS, especially H 2 O 2 , can act together with ET in a selfamplifying feedback loop where ET induces H 2 O 2 accumulation, which then enhances the production of ET, and thus, high level of H 2 O 2 and ET may initiate detrimental processes such as leaf senescence or chlorosis under heat stress (Wi et al 2010;Xia et al 2015). At the same time, ET could also regulate ROS metabolism by modulating antioxidant enzymes (Takács et al 2018). In addition, ET-induced ERFs play a role in redox regulation and oxidative stress induces ERFs (Müller and Munné-Bosch 2015).…”

Section: Involvement Of Ethylene In Ros and Rns-signaling And Defense Systems Under Heat Stressmentioning

confidence: 99%

Ethylene involvement in the regulation of heat stress tolerance in plants

et al. 2021

View full text Add to dashboard Cite

Because of the rise in global temperature, heat stress has become a major concern for crop production. Heat stress deteriorates plant productivity and alters phenological and physiological responses that aid in precise monitoring and sensing of mildto-severe transient heat stress. Plants have evolved several sophisticated mechanisms including hormone-signaling pathways to sense heat stimuli and acquire heat stress tolerance. In response to heat stress, ethylene, a gaseous hormone, is produced which is indispensable for plant growth and development and tolerance to various abiotic stresses including heat stress. The manipulation of ethylene in developing heat stress tolerance targeting ethylene biosynthesis and signaling pathways has brought promising out comes. Conversely increased ethylene biosynthesis and signaling seem to exhibit inhibitory effects in plant growth responses from primitive to maturity stages. This review mainly focuses on the recent studies of ethylene involvement in plant responses to heat stress and its functional regulation, and molecular mechanism underlying the plant responses in the mitigation of heat-induced damages. Furthermore, this review also describes the crosstalk between ethylene and other signaling molecules under heat stress and approaches to improve heat stress tolerance in plants.

show abstract

“…The PCR reaction mixture contained 10 ng cDNA template, 400–400 nM forward and reverse primers, 5 µL of Maxima SYBR Green qPCR Master Mix (2X) (Thermo Scientific, Waltham, MA, USA) and nuclease-free water in 10 μL volume (denaturation at 95 °C for 7 min, followed by 40 cycles of denaturation at 95 °C for 15 s and annealing extension at 60 °C for 60 s). qTOWER Software 2.2 (Analytik Jena, Jena, Germany) was used to analyse the data [ 50 ]. Tomato 18S rRNA and elongation factor-1α subunit were applied as reference genes, and the data were calculated by the 2 (−∆∆Ct) formula [ 51 ].…”

Section: Methodsmentioning

confidence: 99%

Effects of Jasmonic Acid in ER Stress and Unfolded Protein Response in Tomato Plants

et al. 2020

Self Cite

View full text Add to dashboard Cite

Endoplasmic reticulum (ER) stress elicits a protective mechanism called unfolded protein response (UPR) to maintain cellular homeostasis, which can be regulated by defence hormones. In this study, the physiological role of jasmonic acid (JA) in ER stress and UPR signalling has been investigated in intact leaves of tomato plants. Exogenous JA treatments not only induced the transcript accumulation of UPR marker gene SlBiP but also elevated transcript levels of SlIRE1 and SlbZIP60. By the application of JA signalling mutant jai1 plants, the role of JA in ER stress sensing and signalling was further investigated. Treatment with tunicamycin (Tm), the inhibitor of N-glycosylation of secreted glycoproteins, increased the transcript levels of SlBiP. Interestingly, SlIRE1a and SlIRE1b were significantly lower in jai1. In contrast, the transcript accumulation of Bax Inhibitor-1 (SlBI1) and SlbZIP60 was higher in jai1. To evaluate how a chemical chaperone modulates Tm-induced ER stress, plants were treated with sodium 4-phenylbutyrate, which also decreased the Tm-induced increase in SlBiP, SlIRE1a, and SlBI1 transcripts. In addition, it was found that changes in hydrogen peroxide content, proteasomal activity, and lipid peroxidation induced by Tm is regulated by JA, while nitric oxide was not involved in ER stress and UPR signalling in leaves of tomato.

show abstract

H2O2 homeostasis in wild-type and ethylene-insensitive Never ripe tomato in response to salicylic acid treatment in normal photoperiod and in prolonged darkness

Cited by 25 publications

References 56 publications

Salicylic acid-induced ROS production by mitochondrial electron transport chain depends on the activity of mitochondrial hexokinases in tomato (Solanum lycopersicum L.)

Salicylic acid-induced ROS production by mitochondrial electron transport chain depends on the activity of mitochondrial hexokinases in tomato (Solanum lycopersicum L.)

Ethylene involvement in the regulation of heat stress tolerance in plants

Effects of Jasmonic Acid in ER Stress and Unfolded Protein Response in Tomato Plants

Contact Info

Product

Resources

About