H2O2 Induces a Transient Multi-phase Cell Cycle Arrest in Mouse Fibroblasts through Modulating Cyclin D and p21Cip1 Expression

Uncoupling protein-2 deficiency promotes oxidant stress and delays liver regeneration in mice

“…36 As shown recently, high levels of ROS may stimulate p21 expression. 6 It is conceivable that increased ROS production in UCP2 Ϫ/Ϫ liver remnants may lead to increased p21 expression, thus accounting for the delay in liver regeneration. Indeed, we detected higher amounts of p21 in UCP2 Ϫ/Ϫ liver remnants by 12 hours after partial hepatectomy with a peak in p21 expression seen a full day earlier than in UCP2 ϩ/ϩ controls (Fig.…”

“…4,5 ROS are known to mediate cell growth arrest 5 and activate proteins that inhibit the cell cycle. 6,7 Therefore, ROS production in the liver remnant is likely to have a role in the negative control of regeneration.…”

Oxidative stress-related mechanisms are associated with xenobiotics exerting excess toxicity to Fanconi anemia cells.

“…This observation fits well with the previously observed up-regulation of apoptotic regulatory p53 protein and cyclin B proteins, inactivation of CDC2 kinase, and cell-cycle arrest, which follows exposure to low doses of cross-linking agents in FA cells (Kruyt et al 1996(Kruyt et al , 1997Kupfer and D'Andrea 1996). Also p53, cyclin B, and CDC2 kinase are known to be involved in oxidative stress pathways (Barnouin et al 2002;Chen et al 2002;Chuang et al 2002).…”

Control of Trx1 redox state modulates protection against methyl methanesulfonate-induced DNA damage via stabilization of p21

“…Mild oxidative stimulation was shown to cause rapid nuclear export and a transient decrease in p21 protein levels (27), while p21 protein levels increased in response to sublethal doses of H 2 O 2 (29,30), inducing G2/M-phase and multi-phase cell cycle arrest in human lung cancer cells (29). This study further found that MMS treatment reduced p21 protein levels, while Trx1 enhanced the stability of p21 protein.…”