H2O2 priming induces proteomic responses to defense against salt stress in maize

Araújo, Gyedre dos Santos; Lopes, Lineker de Sousa; Paula-Marinho, Stelamaris de Oliveira; Mesquita, Rosilene Oliveira; Nagano, Celso Shiniti; Vasconcelos, Fábio Perdigão; Carvalho, Humberto Henrique de; Moura, Arlindo de Alencar Araripe; Marques, Elton Camelo; Gomes-Filho, Enéas

doi:10.1007/s11103-021-01127-x

Cited by 9 publications

(16 citation statements)

References 73 publications

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“…Therefore, it could be inferred that exogenous H 2 O 2 regulates the gaseous exchange under stress (single or combined) and improves photosynthetic activity, which may result in better quinoa performance. Previously, Araujo et al (2021) and Habib et al (2021), working respectively on maize ( Zea mays ) and wheat ( Triticum aestivum ), revealed that exogenous H 2 O 2 improved crop growth under salinity, possibly due to metabolites modulation and increased antioxidant activities. As stated in the previous section, the Pichaman genotype showed an impaired enzymatic antioxidant system with significantly decreased SOD and POD activities under single stress (Figure 5).…”

Section: Discussionmentioning

confidence: 99%

“…It has been extensively reported that excessive ROS production (i.e., H 2 O 2 and

{normalO}_{2}^{\cdot -}

) under drought and salt stress can cause redox imbalance in various crops plants (Araujo et al, 2021; Dugasa et al, 2021; Habib et al, 2021; Hossain et al, 2015). ROS are highly cytotoxic and can damage the cellular membranes, causing lipid peroxidation and leading to growth inhibition in numerous plant species (Choudhury et al, 2017; Hossain et al, 2015; Iqbal et al, 2018b).…”

Section: Discussionmentioning

confidence: 99%

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Salinity and exogenous H₂O₂ improve gas exchange, osmoregulation, and antioxidant metabolism in quinoa under drought stress

Iqbal,

Yaning,

Waqas

et al. 2023

Physiologia Plantarum

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Climate change‐induced concurrent drought and salinity stresses significantly threaten global crop yields, yet the physio‐biochemical responses to combined stress in quinoa remain elusive. This study evaluated quinoa responses under four growth conditions: well‐watered, drought stress, salt stress, and drought + salt stress with (15 mM) or without (0 mM) exogenous hydrogen peroxide (H2O2) application. All examined stresses (alone or in combination) reduce quinoa growth and net photosynthesis, although salt stress was found to be less destructive than drought and combined stress. Strikingly, superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), stomatal conductance (gs), photosynthetic rate (PN), K+ uptake, shoot height, shoot fresh, and dry weight were increased by 46.1%, 22.2%, 101.6%, 12.9%, 12.1%, 22.4%, 7.1%, 14%, and 16.4%, respectively, under combined stress compared to drought alone. In addition, exogenous H2O2 effectively improved gaseous exchange, osmolytes' accumulation, and antioxidant activity, resulting in reduced lipid peroxidation, which eventually led to higher plant growth under all coercive conditions. The principle component analysis (PCA) indicated a strong positive correlation between antioxidant enzymes and inorganic ions, which contributed efficiently to osmotic adjustment, particularly under conditions of salinity followed by combined stress. In short, in combination, salt stress has the potential to mitigate drought‐induced injuries by promoting the absorption of inorganic solutes for osmoregulation in quinoa plants. Furthermore, exogenous application of H2O2 could be opted to enhance quinoa performance to increase its tolerance mechanism against drought and salinity, even under combined stress.

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Section: Discussionmentioning

confidence: 99%

“…It has been extensively reported that excessive ROS production (i.e., H 2 O 2 and

{normalO}_{2}^{\cdot -}

Section: Discussionmentioning

confidence: 99%

Salinity and exogenous H₂O₂ improve gas exchange, osmoregulation, and antioxidant metabolism in quinoa under drought stress

Iqbal,

Yaning,

Waqas

et al. 2023

Physiologia Plantarum

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“…Although H 2 O 2 is not a free radical, it is the source of many oxygen-containing free radicals and the conversion of other ROS. H 2 O 2 is an important signaling molecule in plant cells, capable of mediating the response of plants to stress [7]. H 2 O 2 may mediate ABA-induced stomatal closure by controlling potassium channels on the cytoplasmic membrane and activating calcium channels [8].…”

Section: Introductionmentioning

confidence: 99%

Exogenous ABA Enhances the Antioxidant Defense System of Maize by Regulating the AsA-GSH Cycle under Drought Stress

Jiang

Zhu

et al. 2022

Sustainability

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When drought occurs during the maize-filling period, the probability of yield decline increases. Abscisic acid (ABA) plays a regulatory role in physiological and metabolic activities during plant development. However, its effect on the antioxidant system of maize leaves during the grain-filling stage is unclear. Maize plants (Zhengdan958) were used as an experimental material, and ABA was sprayed on the leaves during the grain-filling stage. The plants were placed under drought conditions to analyze the relationship between the ascorbate-glutathione (AsA-GSH) cycle and hydrogen peroxide (H2O2) removal. Exogenous ABA significantly reduced the malondialdehyde content, relative electrolyte leakage, and H2O2 under drought stress. This is similar to the exogenous ABA effect on the AsA-GSH cycle. Exogenous ABA upregulated the transcription of related genes and alleviated the inhibition of drought stress on the monodehydroascorbate reductase and dehydroascorbate reductase activities, thereby further increasing the ascorbate peroxidase and glutathione reductase activities. It contributed to an increase in the AsA and GSH levels and inhibited the decrease in the AsA/dehydroascorbic acid and GSH/oxidized glutathione ratios. Therefore, exogenous ABA plays an important role in improving the antioxidant capacity and drought resistance physiology of maize by enhancing antioxidant enzyme activity and stabilizing the AsA and GSH redox state.

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“…Dry seeds contain all the components required for germination and seedling establishment until the seedlings reach an autotrophic state ( Bai et al, 2020 ). Proteins related to energy metabolism may show increased abundance by seed priming ( Araujo et al, 2021 ). Carrot seeds have an endosperm with a high lipid content (mainly in the form of triacylglycerol), which is degraded to provide carbon skeleton and energy for seedling growth during germination ( Ngo-Duy et al, 2009 ; Pandey et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

“…Proteomic techniques can effectively identify the characteristics of protein composition, which is an effective way to explore the molecular physiological basis of seed priming ( Macovei et al, 2017 ; Marthandan et al, 2020 ). Numerous studies have used proteomic techniques to further uncover the molecular mechanisms of seed priming such as durum wheat ( Fercha et al, 2013 ), maize ( Araujo et al, 2021 ), rapeseed ( Kubala et al, 2015 ) and alfalfa ( Yacoubi et al, 2013 ), which focused on key proteins involved in reserve mobilization, protein metabolism, cell cycle and antioxidant protection through the analysis of the differentially abundant proteins (DAPs). However, as a representative of Apiaceae crops, the seeds of carrot come from cremocarp, so it is worth exploring whether the response of carrot seeds to priming is also related to these processes.…”

Section: Introductionmentioning

confidence: 99%

Carrot (Daucus carota L.) Seed Germination Was Promoted by Hydro-Electro Hybrid Priming Through Regulating the Accumulation of Proteins Involved in Carbohydrate and Protein Metabolism

Zhao

Zou

Jia³

et al. 2022

Front. Plant Sci.

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Asynchronized and non-uniform seed germination is causing obstacles to the large-scale cultivation of carrot (Daucus carota L.). In the present study, the combination of high voltage electrostatic field treatment (EF) with hydropriming (HYD), namely hydro-electro hybrid priming (HEHP), significantly improved all germination indicators of carrot seeds, and the promoting effect was superior to that of the HYD treatment. A tandem mass tags (TMT)-based proteomic analysis identified 4,936 proteins from the seeds, and the maximum number of differentially abundant proteins (DAPs) appeared between CK and HEHP. KEGG analysis revealed that the upregulated DAPs were mainly enriched in the pathways related to protein synthesis and degradation such as “ribosome” and “proteasome,” while the downregulated DAPs were mainly enriched in photosynthesis-related pathways. Furthermore, the maximum DAPs were annotated in carbohydrate metabolism. Some proteins identified as key enzymes of the glyoxylate cycle, the tricarboxylate cycle, glycolysis and the pentose phosphate pathway showed enhanced abundance in priming treatments. The activities of several key enzymes involved in carbohydrate metabolism were also enhanced by the priming treatments, especially the HEHP treatment. Real-time quantitative PCR (qRT-PCR) analysis revealed that the effect of priming is mainly reflected before sowing. In conclusion, the optimal effect of HEHP is to regulate the synthesis and degradation of proteins in seeds to meet the requirements of germination and initiate the utilization of seed storage reserves and respiratory metabolism. The present work expanded the understanding of the response mechanism of carrot seed germination to priming and the biological effects of high voltage electrostatic field.

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H2O2 priming induces proteomic responses to defense against salt stress in maize

Cited by 9 publications

References 73 publications

Salinity and exogenous H₂O₂ improve gas exchange, osmoregulation, and antioxidant metabolism in quinoa under drought stress

Salinity and exogenous H₂O₂ improve gas exchange, osmoregulation, and antioxidant metabolism in quinoa under drought stress

Exogenous ABA Enhances the Antioxidant Defense System of Maize by Regulating the AsA-GSH Cycle under Drought Stress

Carrot (Daucus carota L.) Seed Germination Was Promoted by Hydro-Electro Hybrid Priming Through Regulating the Accumulation of Proteins Involved in Carbohydrate and Protein Metabolism

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H2O2 priming induces proteomic responses to defense against salt stress in maize

Cited by 9 publications

References 73 publications

Salinity and exogenous H2O2 improve gas exchange, osmoregulation, and antioxidant metabolism in quinoa under drought stress

Salinity and exogenous H2O2 improve gas exchange, osmoregulation, and antioxidant metabolism in quinoa under drought stress

Exogenous ABA Enhances the Antioxidant Defense System of Maize by Regulating the AsA-GSH Cycle under Drought Stress

Carrot (Daucus carota L.) Seed Germination Was Promoted by Hydro-Electro Hybrid Priming Through Regulating the Accumulation of Proteins Involved in Carbohydrate and Protein Metabolism

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Salinity and exogenous H₂O₂ improve gas exchange, osmoregulation, and antioxidant metabolism in quinoa under drought stress

Salinity and exogenous H₂O₂ improve gas exchange, osmoregulation, and antioxidant metabolism in quinoa under drought stress