H2O2 production and gene expression of antioxidant enzymes in kimchi cabbage (Brassica rapa var. glabra Regel) seedlings regulated by plant development and nitrosative stress-triggered cell death

Enhanced Tolerance of Chinese Cabbage Seedlings Mediated by Bacillus aryabhattai H26-2 and B. siamensis H30-3 against High Temperature Stress and Fungal Infections

“…PCR amplifications were carried out in 50-μl reaction mixture. PCR products were subjected to gel electrophoresis containing 1.4% agarose and RedSafe TM Nucleic Acid Staining Solution (iNtRON Biotechnology, Republic of Korea), visualised by UV-illumination and photographed ( Kim et al, 2015b ).…”

“…Total RNA isolation and semi-quantitative RT-PCR analysis were performed with the third true leaves from three-week-old Chinese cabbage seedlings treated with or without B. siamensis H30-3 according to the methods of our previous studies ( Kim et al, 2015b ; Lee and Hong, 2014 ). Total RNA was isolated from the leaf tissues using RiboEx solution (GeneAll Biotechnology, Co., Ltd., Seoul, Republic of Korea), and two micrograms of the total RNA was converted to cDNA using Superscript III reverse transcriptase and oligo (dT) 20 primer according to the manufacturer’s instructions (Thermo Fisher Scientific, MA, USA).…”

Hydrogen Peroxide Signaling in Plant Development and Abiotic Responses: Crosstalk with Nitric Oxide and Calcium

“…For example, H 2 O 2 as signaling molecule may participate in nitrosative stress-triggered cell death in kimchi cabbage ( Brassica rapa var. glabra Regel) seedlings (Kim et al, 2015 ). Also, Li et al ( 2015 ) suggested that H 2 O 2 is involved in signaling crosstalk between NO and hydrogen sulfide (H 2 S) to induce thermotolerance in maize seedlings.…”