H2S Emission and Microbial Community of Chicken Manure and Vegetable Waste in Anaerobic Digestion: A Comparative Study

Abstract

In order to solve the problem of H2S corrosion in biogas utilization, it is necessary to understand the characteristics and mechanisms of H2S production in chicken manure anaerobic digestion (CMAD) and vegetable waste anaerobic digestion (VWAD). In this study, lab-scale batch tests of CMAD and VWAD were conducted for 67 days at 35 °C. The results showed that sulfide was found to be the major form of sulfur in CMAD (accounting for 90%) and VWAD (70%). The average concentration of H2S was 198 ± 79 ppm in CMAD and 738 ± 210 ppm in VWAD. Moreover, 81% of total H2S was produced at 20 days of methane production in CMAD, but 80% of total H2S was produced in the first day in VWAD because of the rapid production of biogas and fermentation acidification. The sulfide ion equilibrium model could universally and feasibly predict the H2S production in CMAD and VWAD. The abundance of Firmicutes, Bacteroidetes, Proteobacteria and Euryarchaeota accounted for about 95% of the total microbes in both CMAD and VWAD; the influence of the fermentation stage on the microbial community was greater than that of the difference between CM and VW; the abundance of SRB was 0.01~0.07%, while that concerning organosulfur compounds fermentation was 22.8~30.5%. This study indicated that the H2S concentration of CMAD biogas was more than five times that of VWAD because CM is alkalescent but VW is acidic.

Bibliographical review on assessment methodologies to evaluate the electrical energy recovered from biomass conversion technologies

“…While CO2 does not contribute at any time to the degradation of the mechanical system of the site, H2S causes corrosion and mechanical wear increasing maintenance costs drastically, thus posing occupational health and safety problems from its colorless nature, flammable and extremely dangerous [41]. In general, the H2S content in biogas from the anaerobic digestion of municipal organic waste is 500-4000 ppm, however a large value in H2S confirms the existence of organic sulfur compounds in the in buried waste [42].…”

“…The stability and progress of landfill fermentation are key indicators of the degradation of fermentable organic matter in landfill, whose CH4/CO2 content ratio must be less than or equal to two [40,41,42]. Adopting the most common and fastest solutions for abnormal values detected by the biogas analyzer will improve the operating parameters of the landfill site.…”

Microbial Growth Rate Kinetics in Biogas Production by Anaerobic Digestion of Chicken Manure

“…In CM, there are different types of micro-organisms, namely, Salmonella spp., Escherichia coli (E. coli), and Cryptosporidium, to mention a few (Nauanova et al, 2020;Sule et al, 2019;Tian et al, 2023). During anaerobic batch fermentation of CM, these microorganisms grow under a variety of physical, chemical, and nutritional conditions.…”