H3K36 methyltransferase SDG708 enhances drought tolerance by promoting abscisic acid biosynthesis in rice

Chromatin remodelers act in an ATP-dependent manner to modulate chromatin structure and thus genome function. Here, we report that the Arabidopsis (Arabidopsis thaliana) remodeler CHROMATIN REMODELING19 (CHR19) is enriched in gene body regions, and its depletion causes massive changes in nucleosome position and occupancy in the genome. Consistent with these changes, an in vitro assay verified that CHR19 can utilize ATP to slide nucleosomes. A variety of inducible genes, including several important genes in the salicylic acid (SA) and jasmonic acid (JA) pathways, were transcriptionally up-regulated in the chr19 mutant under normal growth conditions, indicative of a role of CHR19 in transcriptional repression. In addition, the chr19 mutation triggered higher susceptibility to the JA pathway-defended necrotrophic fungal pathogen Botrytis cinerea, but did not affect the growth of the SA pathway-defended hemibiotrophic bacterial pathogen Pseudomonas syringae pv. tomato DC3000. Expression of CHR19 was tissue-specific and inhibited specifically by SA treatment. Such inhibition significantly decreased the local chromatin enrichment of CHR19 at the associated SA pathway genes, which resulted in their full activation upon SA treatment. Overall, our findings clarify CHR19 to be a novel regulator acting at the chromatin level to impact the transcription of genes underlying plant resistance to different pathogens.

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“…The CLUSTER PROFILER package for R software was used for GO analysis as previously described (Chen et al, 2021).…”

Section: Transcription and Transcriptome Analysesmentioning

confidence: 99%

Arabidopsis CHROMATIN REMODELING 19 acts as a transcriptional repressor and contributes to plant pathogen resistance

et al. 2021

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“…According to RNA-Seq analysis, SDG102 is necessary for the proper expression of numerous genes involved in diverse biological processes. In addition to flowering, ASH1 family proteins in rice and Arabidopsis such as SDG26 and SDG8 in Arabidopsis and SDG708 and SDG725 in rice also affect a variety of plant phenotypes reported in previous studies [28][29][30]33,40,43]. RNA-Seq analyses demonstrated that DEGs between the sdg102 mutants and the WT are distributed across functional categories such as hormone signal transduction, cell composition of the nucleosome and histone modification, carbohydrate metabolism, and response to stress.…”

Section: Discussionmentioning

confidence: 73%

“…In addition to controlling the flowering time, SDG708 also acts as a positive regulator of drought tolerance. The overexpression of SDG708 increased the grain yield and drought tolerance under normal and drought stress conditions in rice [43]. The overexpression of rice SDG736 in A. thaliana, the closest rice homolog of Arabidopsis SDG4, resulted in early flowering.…”

Section: Introductionmentioning

confidence: 97%

SDG102, a H3K36-Methyltransferase-Encoding Gene, Plays Pleiotropic Roles in Growth and Development of Maize (Zea mays L.)

Sun

Wang

et al. 2022

IJMS

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Although histone lysine methylation has been studied in thale cress (Arabidopsis thaliana (L.) Heynh.) and rice (Oryza sativa L.) in recent years, its function in maize (Zea mays L.) remains poorly characterized. To better understand the function of histone lysine methylation in maize, SDG102, a H3 lysine 36 (H3K36) methylase, was chosen for functional characterization using overexpressed and knockout transgenic plants. SDG102-deficiency in maize caused multiple phenotypes including yellow leaves in seedlings, late-flowering, and increased adult plant height, while the overexpression of SDG102 led to reduced adult plant height. The key flowering genes, ZCN8/ZCN7 and MADS4/MADA67, were downregulated in SDG102-deficient plants. Chromatin immunoprecipitation (ChIP) experiments showed that H3 lysine 36 trimethylation (H3K36me3) levels were reduced at these loci. Perturbation of SDG102 expression caused the misexpression of multiple genes. Interestingly, the overexpression or knockout of SDG102 also led to genome-wide decreases and increases in the H3K36me3 levels, respectively. Together, our results suggest that SDG102 is a methyltransferase that catalyzes the trimethylation of H3K36 of many genes across the maize genome, which are involved in multiple biological processes including those controlling flowering time.

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“…OsSDG708 , encoding a putative H3K36 methyltransferase, positively regulates drought tolerance by directly targeting and activating the crucial ABA biosynthesis genes OsNCED3 and OsNCED5 in rice. Overexpression of OsSDG708 enhances the drought tolerance and increases the grain yield either, highlighting its potential role epigenetic regulation ( Chen et al, 2021b ). In dehydrated maize plants, higher active methylation (H3K36me3 and H3K4me3) and lower repressive methylation (H3K27me3) are deposited in the loci of antisense transcripts, indicating biogenesis of small RNAs might be regulated by histone methylation in drought response ( Xu et al, 2017 ).…”

Section: Two Dynamic Counterparts In Stress-responsive Gene Transcrip...mentioning

confidence: 99%

“…Later in another study, the authors found that FLD/SDG26/LD interacts with R-loop to slow both the transcription initiation and the elongation ( Xu et al, 2021 ). Since the ASH-group HKMTs are involved in drought tolerance ( Chen et al, 2021b ), the output when multiple epigenetic mechanisms are combinatorially involved needs to be reconsidered.…”

Section: Cross-talks Of Histone (De)methylation In Response To Osmoti...mentioning

confidence: 99%

Methylation hallmarks on the histone tail as a linker of osmotic stress and gene transcription

2022

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Plants dynamically manipulate their gene expression in acclimation to the challenging environment. Hereinto, the histone methylation tunes the gene transcription via modulation of the chromatin accessibility to transcription machinery. Osmotic stress, which is caused by water deprivation or high concentration of ions, can trigger remarkable changes in histone methylation landscape and genome-wide reprogramming of transcription. However, the dynamic regulation of genes, especially how stress-inducible genes are timely epi-regulated by histone methylation remains largely unclear. In this review, recent findings on the interaction between histone (de)methylation and osmotic stress were summarized, with emphasis on the effects on histone methylation profiles imposed by stress and how histone methylation works to optimize the performance of plants under stress.

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H3K36 methyltransferase SDG708 enhances drought tolerance by promoting abscisic acid biosynthesis in rice

Cited by 29 publications

References 87 publications

Arabidopsis CHROMATIN REMODELING 19 acts as a transcriptional repressor and contributes to plant pathogen resistance

Arabidopsis CHROMATIN REMODELING 19 acts as a transcriptional repressor and contributes to plant pathogen resistance

SDG102, a H3K36-Methyltransferase-Encoding Gene, Plays Pleiotropic Roles in Growth and Development of Maize (Zea mays L.)

Methylation hallmarks on the histone tail as a linker of osmotic stress and gene transcription

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