H7, a protein kinase C inhibitor, increases the glutathione content of neuroblastoma cells

Abstract

It is shown that the intracellular glutathionc (GSH) concentration of neuroblastoma-h cells in culture increases with 3 maximum at 24 h after starting treatment with I-(S-isoquinolinylsulfonyl)-2-methylpipcrazine (H7), an inhibitor of protein kinase C (PKC). Other inhibitors of this and other protein hinases, e.g. sphingosinc, staurosporinc, and HA 1004, at the eoncentr?tions tested, bad a less mark& or negligible cffcct on intracellular GSH conccntrntion. 12.0.Tetradccanoylphorbol-13.acetate (TPA) was also tested and jhowcd :lo sigilicant effect 24 h after addition.