HA1-2-fljB Vaccine Induces Immune Responses against Pandemic Swine-Origin H1N1 Influenza Virus in Mice

Kang, Xiangtao; Yang, Yun; Jiao, Yang; Song, Hongqin; Song, Li; Xiong, Dan; Wu, Li; Pan, Zhiming; Jiao, Xinan

doi:10.1159/000448895

Cited by 4 publications

(2 citation statements)

References 39 publications

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“…Then, these tissue samples were fixed in 10% neutral-buffered formalin. After fixation, the tissues were embedded in paraffin using the conventional method and then stained with H&E as previously described ( Kang et al., 2016 ). Histopathological analyses were performed under a light microscope (Nikon Eclipse Ci-L, Nikon, Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

Safety and protective efficacy of Salmonella Pullorum spiC and rfaH deletion rough mutant as a live attenuated DIVA vaccine candidate

et al. 2022

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Salmonella enterica serovar Pullorum ( S. Pullorum) causes pullorum disease ( PD ), which is an acute systemic disease, in chickens, and leads to serious economic losses in many developing countries because of its high morbidity and mortality rate in young chicks. The live-attenuated vaccine is considered to be an effective measure to control the Salmonella infection. In addition, the DIVA (differentiation of infected and vaccinated animals) feature without the interference of serological monitoring of Salmonella infection is an important consideration in the development of the Salmonella vaccine. In this study, we evaluated the immunogenicity and protective efficacy of a S. Pullorum rough mutant S06004 ΔspiCΔrfaH as a live attenuated DIVA vaccine candidate in chickens. The S06004 ΔspiCΔrfaH exhibited a significant rough lipopolysaccharides ( LPS ) phenotype which was agglutinated with the acriflavine, not with the O 9 mono antibody. Compared to the wild-type, 50% lethal dose (LD 50 ) of the rough mutant increased 100-fold confirmed its attenuation. The mutant strain also showed a decreased bacterial colonization in the spleen and liver. The immunization with the mutant strain had no effect on the body weight and no tissue lesions were observed in the liver and spleen. The high level of the S. Pullorum-specific IgG titers in the serum indicated that significant humoral immune responses were induced in the immunization group. The cellular immune responses were also elicited from the analysis of lymphocyte proliferation and expression of cytokines in the spleen. In addition, the S06004 ΔspiCΔrfaH immunized group exhibited a negative response for the serological test, while the wild-type S06004 infection group was strongly positive for the serological test showing a DIVA capability. The survival rates in the vaccinated chickens were 87% after intramuscular challenge with wild-type S. Pullorum, while the survival rates were 20% in the control groups. Overall, these results have demonstrated that the rough mutant S06004 ΔspiCΔrfaH strain can be developed as an efficient live attenuated DIVA vaccine candidate to control the systemic S. Pullorum infection without the interference of salmonellosis monitoring program in poultry.

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Section: Methodsmentioning

confidence: 99%

Safety and protective efficacy of Salmonella Pullorum spiC and rfaH deletion rough mutant as a live attenuated DIVA vaccine candidate

et al. 2022

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“…Western blotting was used to assess IKKγ expression in BMDCs, as described previously ( 16 ). Lysates from transfected BMDCs following 24 h of treatment with flagellin or medium alone were denatured and subjected to SDS-PAGE.…”

Section: Methodsmentioning

confidence: 99%

MicroRNA-5112 Targets IKKγ to Dampen the Inflammatory Response and Improve Clinical Symptoms in Both Bacterial Infection and DSS-Induced Colitis

et al. 2022

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Inflammation is a double-edged sword that can be induced by various PAMPs, resulting in the control of infection by invading pathogens or injuries. The inflammatory response requires strict and precise control and regulation. MicroRNAs (miRNAs) are small non-coding RNA molecules that regulate gene expression via translational inhibition or mRNA degradation. However, the role of miRNAs in inflammation induced by flagellin (ligand of TLR5) has yet to be fully determined. In this study, we identified differentially expressed miRNAs in murine bone marrow-derived dendritic cells (BMDCs) between flagellin treatment and medium alone using miRNA microarray. We found that flagellin stimulation downregulated miR-5112 expression in BMDCs and spleen DCs in vitro and in vivo. The overexpression of miR-5112 decreased inflammatory cytokine production, accompanied by a reduction of IKKγ in flagellin-stimulated BMDCs. We demonstrated that miR-5112 could directly target IKKγ to inhibit inflammatory cytokine production. Furthermore, miR-5112 inhibited the inflammatory response induced by flagellin or Salmonella infection in vivo. Interestingly, miR-5112 could also dampen the inflammatory response and alleviate dextran sulfate sodium (DSS)-induced colitis in C57BL/6 mice. These results suggest that miR-5112 could be a novel therapeutic target for both bacterial infection and DSS-induced colitis model.

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Attachment of flagellin enhances the immunostimulatory activity of a hemagglutinin-ferritin nano-cage

Lee

Kim

et al. 2019

Nanomedicine: Nanotechnology, Biology and Medicine

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HA1-2-fljB Vaccine Induces Immune Responses against Pandemic Swine-Origin H1N1 Influenza Virus in Mice

Cited by 4 publications

References 39 publications

Safety and protective efficacy of Salmonella Pullorum spiC and rfaH deletion rough mutant as a live attenuated DIVA vaccine candidate

Safety and protective efficacy of Salmonella Pullorum spiC and rfaH deletion rough mutant as a live attenuated DIVA vaccine candidate

MicroRNA-5112 Targets IKKγ to Dampen the Inflammatory Response and Improve Clinical Symptoms in Both Bacterial Infection and DSS-Induced Colitis

Attachment of flagellin enhances the immunostimulatory activity of a hemagglutinin-ferritin nano-cage

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