HA1-specific indirect ELISA for serological detection of canine influenza virus H3N2 infection in dogs

Shim, Doo Hee; Kim, Jeong-Ki; Hong, Moongeun; Na, Woonsung; Park, Yong-A; Park, Seong Jun; Song, Daesub; Lee, Jae Myun; Kim, Hye Kwon

doi:10.1016/j.jviromet.2015.01.007

Cited by 1 publication

(1 citation statement)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The wing venous blood samples were collected on days 0, 7, 14, 21, 28, 35, 42 after the first vaccination and then the serum was separated for antibody titer determination. The antibody titers were determined by indirect ELISA [ 23 ]. In briefly, ELISA plates (Greiner Bio-One, Shanghai, China) were coated with 0.1 μg·mL −1 Ptfa protein (or peptide MetII) in cold carbonate buffer and incubated overnight at 4°C.…”

Section: Methodsmentioning

confidence: 99%

The antimicrobial peptide MetchnikowinII enhances Ptfa antigen immune responses against avian <i>Pasteurella multocida</i> in chickens

Niu

Gong

et al. 2023

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

Immunostimulants and vaccines are the main means for controlling infectious diseases and searching highly effective and low toxic immunestimulants has always been the focus of researchers. The MetchnikowinII (MetII) had been expressed by us and exhibited both antibacterial and antifungal activities, in this study, we evaluated its potential for an adjuvant effect. In chickens, antigen-specific immunoglobulin Gs (IgGs) were increased after MetII adjuvanted vaccination using the Ptfa protein. Compared to group Ptfa + iFA, which was only adjuvanted with incomplete Freund’s adjuvant (iFA), the antibody titers of the group Ptfa + iFA + Met20 μg·mL −1 (PFM 20 ) and Ptfa + iFA + Propolis (PFP) significantly increased ( P <0.05). Likewise, Interleukin-2 (IL-2) and Interferon-γ (IFN-γ) cytokines in group Ptfa + iFA + Met20 μg·mL −1 (PFM 20 ) and Ptfa + iFA + Propolis (PFP) were significantly higher than those of the other three experimental groups ( P <0.05). The stimulation index (SI) value in chickens of group PFM 20 was significantly higher than that of the other four experimental groups ( P <0.05). Chickens that received MetII adjuvanted vaccinations benefitted from higher protection rate (88%) when challenged with Pasteurella multocida ( P. multocida ), which was significantly higher than those of group PF and PFP ( P <0.05). These results suggested that the antimicrobial peptide MetII may play an adjuvant role in the immune response in chickens but need a proper usage, because the higher usage of 40 μg·mL −1 and 60 μg·mL −1 resulted poor effect. Whether MetII could be a potential adjuvant or a biomolecule as part of a complex adjuvant for vaccines needs more experimental evidence, the study still provides an examples for understanding vaccine adjuvants.

show abstract

Section: Methodsmentioning

confidence: 99%