Habitat complexity affects benthic harmful dinoflagellate assemblages in the fringing reef of Rawa Island, Malaysia

Yong, Hwa Lin; Mustapa, Nurin Izzati; Lee, Li Keat; Lim, Zhen Fei; Tan, Toh Hii; Usup, Gires; Gu, Haifeng; Litaker, R. Wayne; Tester, Patricia A.; Lim, Po Teen; Leaw, Chui Pin

doi:10.1016/j.hal.2018.07.009

Cited by 47 publications

(39 citation statements)

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“…cell −1 , respectively. Cell densities were found to be relatively low (never exceeding 100 cells 100 cm −2 ) compared to values as high as 10,000 cells 100 cm −2 reported in Belize [34] or 1200 cells 100 cm −2 in Malaysia [35]. Interpretation of sampling data with limited spatial and temporal scale, as conducted in this study (one sampling event for microalgae), requires cautious judgement considering the known geographical and temporal variability in distributions of Gambierdiscus spp.…”

Section: Discussioncontrasting

confidence: 50%

“…Interpretation of sampling data with limited spatial and temporal scale, as conducted in this study (one sampling event for microalgae), requires cautious judgement considering the known geographical and temporal variability in distributions of Gambierdiscus spp. [35,36]. It highlights the complexity of developing an environmental monitoring strategy for seafood safety and disease management, with a need to conduct concomitant surveillance of toxins in fish for efficient monitoring.…”

Section: Discussionmentioning

confidence: 99%

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Ciguatoxin Occurrence in Food-Web Components of a Cuban Coral Reef Ecosystem: Risk-Assessment Implications

Díaz-Asencio

Clausing

Vandersea

et al. 2019

Toxins

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In Cuba, ciguatera poisoning associated with fish consumption is the most commonly occurring non-bacterial seafood-borne illness. Risk management through fish market regulation has existed in Cuba for decades and consists of bans on selected species above a certain weight; however, the actual occurrence of ciguatoxins (CTXs) in seafood has never been verified. From this food safety risk management perspective, a study site locally known to be at risk for ciguatera was selected. Analysis of the epiphytic dinoflagellate community identified the microalga Gambierdiscus. Gambierdiscus species included six of the seven species known to be present in Cuba (G. caribaeus, G. belizeanus, G. carpenteri, G. carolinianus, G. silvae, and F. ruetzleri). CTX-like activity in invertebrates, herbivorous and carnivorous fishes were analyzed with a radioligand receptor-binding assay and, for selected samples, with the N2A cell cytotoxicity assay. CTX activity was found in 80% of the organisms sampled, with toxin values ranging from 2 to 8 ng CTX3C equivalents g −1 tissue. Data analysis further confirmed CTXs trophic magnification. This study constitutes the first finding of CTX-like activity in marine organisms in Cuba and in herbivorous fish in the Caribbean. Elucidating the structure-activity relationship and toxicology of CTX from the Caribbean is needed before conclusions may be drawn about risk exposure in Cuba and the wider Caribbean. Key Contribution:This study constitutes a first investigation of fish contamination with ciguatoxines (CTXs) in Cuba. CTXs were present at all levels of the food web, including in widespread Caribbean herbivores generally considered safe for consumption. We discuss remaining analytical challenges to assess exposure and hence to develop effective management strategies for the associated foodborne disease. Supplementary Materials:The following are available online at http://www.mdpi.com/2072-6651/11/12/722/s1: Figure S1: Toxicity of sampled fish and invertebrate specimens. Horizontal bars represent median RBA analysis among specimens, boxes extend from the 25th to 75th percentiles and whiskers represent min to max specimens RBA values. The horizontal line corresponds to the RBA limit of quantification (LOQ = 1.5 ng CTX3C equiv. g-1). RBA-specimens are not shown. Shaded areas indicate fish species banned by Cuban regulation. Author Contributions: Conceptualization, C.M.A.-H.; Data curation, L.

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Section: Discussioncontrasting

confidence: 50%

Section: Discussionmentioning

confidence: 99%

Ciguatoxin Occurrence in Food-Web Components of a Cuban Coral Reef Ecosystem: Risk-Assessment Implications

Díaz-Asencio

Clausing

Vandersea

et al. 2019

Toxins

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“…Habitat preference likely varies between the two species, as the former was found attached to macroalgae and the latter was found in sediments (own observation). Several studies have documented the benthic distribution of Prorocentrum (Vila et al, 2001;Okolodkov et al, 2007Okolodkov et al, , 2014Yong et al, 2018). But as yet, very few studies focused on the ecological adaptation and habitat preference of benthic Prorocentrum at interspecific levels, this is not a topic to cover in this study but will be a subject to explore in the future.…”

Section: Taxonomic Remarks Of Two Toxic Prorocentrum Species Found Inmentioning

confidence: 89%

Taxonomy and toxicity of Prorocentrum from Perhentian Islands (Malaysia), with a description of a non-toxigenic species Prorocentrum malayense sp. nov. (Dinophyceae)

Lim

Luo²,

Lee

et al. 2019

Harmful Algae

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A B S T R A C TThirteen isolates of Prorocentrum species were established from the coral reefs of Perhentian Islands Marine Park, Malaysia and underwent morphological observations and molecular characterization. Six species were found: P. caipirignum, P. concavum, P. cf. emarginatum, P. lima, P. mexicanum and a new morphotype, herein designated as P. malayense sp. nov. Prorocentrum malayense, a species closely related to P. leve, P. cf. foraminosum, P. sp. aff. foraminossum, and P. concavum (Clade A sensu Chomérat et al. 2018), is distinguished from its congeners as having larger thecal pore size and a more deeply excavated V-shaped periflagellar area. Platelet arrangement in the periflagellar area of P. malayense is unique, with the presence of platelet 1a and 1b, platelet 2 being the most anterior platelet, and a broad calabash-shaped platelet 3. The species exhibits consistent genetic sequence divergences for the nuclear-encoded large subunit ribosomal RNA gene (LSU rDNA) and the second internal transcribed spacer (ITS2). The phylogenetic inferences further confirmed that it represents an independent lineage, closely related to species in Clade A sensu Chomérat et al. Pairwise comparison of ITS2 transcripts with its closest relatives revealed the presence of compensatory base changes (CBCs). Toxicity analysis showed detectable levels of okadaic acid in P. lima (1.0-1.6 pg cell ˗1 ) and P. caipirignum (3.1 pg cell ˗1 ); this is the first report of toxigenic P. caipirignum in the Southeast Asian region. Other Prorocentrum species tested, including the new species, however, were below the detection limit.

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“…Most research has focused on the environmental variability and host-epiphytic dynamics through the analysis of the experimental responses of benthic dinoflagellates to variations of temperature, irradiance, nutrient concentration and salinity (Bomber et al, 1988;Chinain et al, 1999;Hales et al, 1999;Anderson et al, 2008;Lartigue et al, 2009;Kibler et al, 2015), as well as the substrate preference of some dinoflagellate species (Lobel et al, 1988;Bomber et al, 1989;Nakahara et al, 1996;Parsons and Preskitt, 2007;Parsons et al, 2011;Rains and Parsons, 2015), and the monitoring of dinoflagellate abundance on several substrates and macroalgae (Morton and Faust, 1997;Vila et al, 2001;Okolodkov et al, 2007Okolodkov et al, , 2014Moreira et al, 2012;Cohu et al, 2013). Also, other features such as depth, the water motion and habitat structure have been studied in relation to abundance and occurrence of the benthic dinoflagellates (Richlen and Lobel, 2011;Cohu et al, 2013;Boisnoir et al, 2018;Yong et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Population Dynamics of Benthic-Epiphytic Dinoflagellates on Two Macroalgae From Coral Reef Systems of the Northern Mexican Caribbean

et al. 2018

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In the present study the abundance of epiphytic dinoflagellates was evaluated at two coral reef sites of the natural protected areas, Arrecife de Puerto Morelos and Isla Contoy, located in the northern Mesoamerican Reef System of the Mexican Caribbean. Abundances were monitored from April to December, 2015 on two genera of macroalga belonging to different functional groups: Dictyota and Amphiroa. In general, the total abundance of dinoflagellates was higher in Puerto Morelos on both macroalgae. Ostreopsis cf. marina and O. heptagona were the dominant species. Relative abundance of these species varied from 8 to 99% of total epiphytic dinoflagellates. Maximum abundances at Puerto Morelos were registered in April, with 33,801 cells•g −1 on Dictyota and 6,264 cells•g −1 on Amphiroa. In Isla Contoy the maximum abundance was 16,006 cells•g −1 and it was detected on Dictyota during December. Other dinoflagellate genera were more abundant during the warmer period (May-September) in both locations. Prorocentrum was the second most abundant genus and was represented by six species (P. hoffmannianum, P. lima, P. belizeanum, P. elegans, P. emarginatum, and P. rathymum). The maximum pooled abundance of Prorocentrum species was 4,144 cells•g −1 on Dictyota in August. Coolia spp. did not reach abundances higher than 1,000 cells•g −1 and Gambierdiscus spp. only exceeded 100 cells•g −1 during August. Mean water temperature in Isla Contoy was significantly lower than that of Puerto Morelos during the entire study period. There was a negative correlation between the water temperature and the abundance of O. cf. marina. The dominance of Ostreopsis in the Caribbean is significant because of its capability to produce palytoxin analogs and its potential role in ciguatera fish poisoning outbreaks in the region. This is the first study that reports blooms of Ostreopsis in Mexican Caribbean coral reefs, a fact that emphasizes the significance of this genus at global scale.

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Habitat complexity affects benthic harmful dinoflagellate assemblages in the fringing reef of Rawa Island, Malaysia

Cited by 47 publications

References 80 publications

Ciguatoxin Occurrence in Food-Web Components of a Cuban Coral Reef Ecosystem: Risk-Assessment Implications

Ciguatoxin Occurrence in Food-Web Components of a Cuban Coral Reef Ecosystem: Risk-Assessment Implications

Taxonomy and toxicity of Prorocentrum from Perhentian Islands (Malaysia), with a description of a non-toxigenic species Prorocentrum malayense sp. nov. (Dinophyceae)

Population Dynamics of Benthic-Epiphytic Dinoflagellates on Two Macroalgae From Coral Reef Systems of the Northern Mexican Caribbean

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