Habitat ecological characteristics and soil fungal community structure of
            <i>Paphiopedilum</i>
            subgenus
            <i>Brachypetalum</i>
            Hallier (Orchidaceae) plants in Southwest China

Tian, Lei; An, Malaviya; Wu, Moxu; Liu, Feng; Zhang, Yang

doi:10.1080/15592324.2023.2227365

Cited by 3 publications

(1 citation statement)

References 61 publications

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“…Research indicates that Ascomycota and Basidiomycota, belonging to the phylum Dikarya, are widely distributed fungal groups in many soil environments and represent dominant fungal phyla in soils (Zhang et al, 2020a). Sordariomycetes and Dothideomycetes classes have been identified as dominant fungal groups in PCH (Tian et al, 2023), suggesting their essential role in the development and growth of PCH.…”

Section: Discussionmentioning

confidence: 99%

Quality analysis and function prediction of soil microbial communities of Polygonatum cyrtonema in two indigenous-origins

Yang,

Wulu

et al. 2024

Front. Microbiol.

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Polygonatum cyrtonema Hua (PCH), as an important economic crop, is used as raw industrial materials and traditional Chinese medicine. There are significant variations in the quality of PCH from different geographical origins. It can be due to the change of the endophytic fungi and soil microbial communities of PCH. Therefore, the aim of this study is to investigate the composition and functional prediction of the main microbial communities in the rhizomes and soil of PCH and explore their impact on medicinal quality. High-throughput sequencing techniques targeting ITS and 16S rDNA were employed to compare the structure and biodiversity differences of endophytic fungi in the rhizomes and soil microbial communities of PCH from 12 different locations in Sichuan and Guangxi province. Heatmap analysis was used for comprehensive statistics and visualization of the richness of rhizome and soil microbial communities from all locations. Venn analysis was conducted to determine the total number of shared fungi between rhizomes and soil, and GraphPad Prism analysis was employed to predict and compare the microbial communities related to phenotypes at the genus level in Sichuan and Guangxi. Tax4Fun and Fungild were used for metabolic function prediction of microbial communities in the rhizomes and soil of PCH. The results revealed the identification of 19,387 bacterial amplicon sequence variants (ASVs) in the rhizomes and 37,990 bacterial ASVs in the soil, with 6,889 shared bacterial ASVs. In addition, 2,948 fungal ASVs were identified in the rhizomes and 8,868 in the soil, with 1,893 shared fungal ASVs. Microbial sequencing results indicated that the fungal communities between soil and rhizomes were mainly composed of Ascomycota and Basidiomycota, while bacterial communities included Proteobacteria, Acidobacteria, Bacteroidota, Gammatimonadota, and Firmicutes. Dominant bacterial groups such as Nitrospira, Acidibacter, and fungal groups including Mortierella, Ceratobasidium, and Fusarium were identified as potential contributors to the observed traits. In the top 15 microbial genera, both Sichuan and Guangxi contain 15 bacterial genera, but there are differences in their abundance. Guangxi has three unique fungal genera, including the genera Scleroderma, Russula, and Gliocladiopsis. On the other hand, Sichuan has the unique fungal genus Chamaeota. The correlation analysis between the microbiota and the chemical content from 12 different collecting spots was performed by GraphPad Prism. Burkholderia-Caballeronia-Paraburkholderia, Acidibacter, and Amycolatopsis show an inverse proportionality to total polysaccharides and saponins, while Enterobacter shows a direct proportionality to total polysaccharides and inverse proportionality to saponins. The metabolism pathways show a significant positive correlation with PCH polysaccharides and saponins. This study provide new insights into the mechanisms underlying the quality differences between the two major indigenous areas.

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Section: Discussionmentioning

confidence: 99%

Quality analysis and function prediction of soil microbial communities of Polygonatum cyrtonema in two indigenous-origins

Yang,

Wulu

et al. 2024

Front. Microbiol.

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Effects of Seafood Mushroom Spent Substrate Solid-State Fermentation Combined with PGPR as a Microbial Fertilizer on the Soil Environment and Growth Promotion of Cenchrus fungigraminus

Li,

Lei,

Qin

et al. 2024

J Soil Sci Plant Nutr

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Purpose Careless burning, discarding, or overfertilization of spent mushroom substrate (SMS) is environmentally harmful. The reuse of SMS resources is a matter of concern. Methods Spent seafood mushroom substrate solid-state fermentation combined with Rhizobium pusense solution was added to soil to evaluate its environmental and Cenchrus fungigraminus seedling growth effects in pot trial experiments. Results The 40% fermented seafood mushroom SMS (fresh weight) with R. pusense treatment had significantly higher total nitrogen (TN), ammonium nitrogen (AMN), organic matter, etc., and decreased soil electrical conductivity (EC) and increased pH compared with other treatment. Soil catalase (CAT), dehydrogenase (DHA), and urease (UE) activities were significantly improved; there were no differences in CAT and UE activities between 40% (T4) and 50% (T5) SMS additions. C. fungigraminus seedling height and leaf length increased gradually and were significantly different from those of the positive control. The 30–50% seafood mushroom SMS treatment increased plant TN by 5.76–10.00% and total phosphorus by 15.44–16.55%. Plant root length and root activity peaked in T4; however, plant split root number and root activity in T5 decreased by 43.96% and 26.97% compared with those in T4. According to Pearson correlation and one-dimensional regression analyses, the seafood mushroom SMS content affected fertility > enzyme activity > plant growth. Principal component analysis and redundancy analysis showed that the effect of the seafood mushroom SMS concentration on the soil environment was mainly reflected in plant aboveground and root activity. Conclusions Under an SMS addition amount of 40%, plant growth-promoting rhizobacteria promoted the growth of C. fungigraminus seedlings to a greater extent.

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Soil Properties and Rhizosphere Microbes Community Structure Reveal Nitrogen Uptake Preferences and Nitrogen Use Efficiency of Two Ecotypes of Paphiopedilum micranthum

Li,

Hu,

Ruan

et al. 2024

Agriculture

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Paphiopedilum micranthum, an IUCN Red List species, is discontinuously distributed in the karst limestone mountain of southwest China and exhibits ecological specialization, typically through lithophytic and terrestrial ecotypes. Whether the distribution of rhizosphere bacteria and fungi in these different habitats is random or reflects soil preferences requires further investigation. A total of 73 samples from the core distribution areas in China, representing all habitats in two sites, were analyzed for soil differences by comparing edaphic properties and microbial community structure based on high-throughput sequencing of bacterial 16S rRNA genes and fungal ITS region sequences, alongside soil physiochemical data. The results showed no significant differences in microbial community richness and diversity across the heterogeneous habitats. However, significant differences in taxa were observed across various habitats. Dominant bacterial phyla included Actinobacteriota, Proteobacteria and Acidobacteriota, with dominant genera such as Crossiella, Pseudonocardia, 67-14, Mycobacterium and RB41. The primary fungal phyla were Basidiomycota and Ascomycota, featuring prominent genera such as Phlegmacium, Archaeorhizomyces, Trechispora, and Lepiota. There were 16 bacterial genera and 13 fungal genera associated with nitrogen transformation and fixation. Alkali-hydrolyzed nitrogen (AN) was identified as a main driver of soil bacterial and fungal community variation. Based on an analysis of soil physicochemical properties, ammonium nitrogen content was consistently higher than nitrate nitrogen across different habitats. Furthermore, across all heterogeneous habitats, P. micranthum showed no significant differences in nitrate nitrogen, ammonium nitrogen, or their ratio. The nitrogen-use efficiency of P. micranthum ranged from 7.73% to 9.87%, with the highest efficiency observed in the terrestrial habitat of Shedu. These results suggest that P. micranthum prefers habitats rich in organic matter and nitrogen, showing a preference for ammonium nitrogen uptake in natural conditions. Heterogeneous habitats affect plant nitrogen-use efficiency as well as changes in microbial community composition.

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Habitat ecological characteristics and soil fungal community structure of Paphiopedilum subgenus Brachypetalum Hallier (Orchidaceae) plants in Southwest China

Cited by 3 publications

References 61 publications

Quality analysis and function prediction of soil microbial communities of Polygonatum cyrtonema in two indigenous-origins

Quality analysis and function prediction of soil microbial communities of Polygonatum cyrtonema in two indigenous-origins

Effects of Seafood Mushroom Spent Substrate Solid-State Fermentation Combined with PGPR as a Microbial Fertilizer on the Soil Environment and Growth Promotion of Cenchrus fungigraminus

Soil Properties and Rhizosphere Microbes Community Structure Reveal Nitrogen Uptake Preferences and Nitrogen Use Efficiency of Two Ecotypes of Paphiopedilum micranthum

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