Habitat ephemerality affects the evolution of contrasting growth strategies and cannibalism in anuran larvae

Abstract

Ephemeral streams are challenging environments for tadpoles; thus adaptive features that increase the survival of these larvae should be favored by natural selection. In this study, we compared the adaptive growth strategies of Bombina orientalis (the oriental fire-bellied toad) tadpoles from ephemeral streams with those of such tadpoles from non-ephemeral streams. Using a common garden experiment, we tested the interactive effects of location (ephemeral vs. non-ephemeral), food availability, and growing density on larval period, weight at metamorphosis, and cannibalism. We found that tadpoles from ephemeral streams underwent a shorter larval period compared with those from non-ephemeral streams but that this difference was contingent on food availability and density. The observed faster growth is likely to be an adaptive response because tadpoles in ephemeral streams experience more biotic/abiotic stressors, such as desiccation risk and limited resources, compared with those in non-ephemeral streams, with their earlier metamorphosis potentially resulting in survival benefits. As a trade-off for their faster growth, tadpoles from ephemeral streams had a lower body weight at metamorphosis compared with those from non-ephemeral streams, but only when food was sufficient. We also found lower cannibalism rates among tadpoles from ephemeral streams, which can be attributed to the indirect fitness costs of cannibalizing their kin. Our study is the first to demonstrate how ephemeral habitats have affected the evolutionary change in cannibalistic behaviors in anurans and provides additional evidence that natural selection has mediated the evolution of growth strategies of tadpoles in ephemeral streams.