Ayer Hitam Utara Forest Reserve (AHUFR) is one of the permanent reserved forests (PRFs) in Johor, which is consisted of lowland and peat swamp ecosystems.In AHUFR, there has been little progress in the study of non-timber species. Non-timber forest products (NTFPs) are forest products other than timber that are obtained from both plants and animals and are vital to all species, including people. Among the objectives of this research project were the creation of a checklist of non-timber species in the AHUFR,the identification of non-timber species in the forest reserve, and the measurement of the diversity of non-timber species in the forest reserve. Survey was conducted in 10m ×5m quadrats, in which three quadrats were established in two different ecosystems, namely site A (lowland forest) and site B (peat swamp). The preliminarystudy documented92individuals from19 families and 33 speciesof non-timber plants.Melastomataceae wasthe most dominant family,whilstClidemia hirta (L.)D.Donwas the most dominant species. Based on the Shannon-Wiener and Margalef indices, lowland forest indicatedhigherdiversity of non-timber species compared to peat swamp forest.