Haemaglutination of Shigella dysenteriae subunit pili protein with anti-haemaglutination of S. dysenteriae subunit pili protein as a molecule adhesion in mouse enterocytes

Sumarno, R. P.; Avanita, A. S.; Winarsih, Sri; Hidayat, Sri Hendrastuti; Nurhidayati, Dwi Yuni

doi:10.5897/ajmr2014.7273

Cited by 5 publications

(2 citation statements)

References 23 publications

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“…The hemagglutinin protein is identical to the adhesion molecule that is responsible for the adhesion of bacteria to the host cell [10]. Therefore, the hemagglutination reaction is a screening tool for bacterial adhesion molecules, which allowed these adhesion molecules to be used as a tool for diagnosis candidates and vaccine component [12,22].…”

Section: Antigen-antibody Reaction By the Methods Of Dot Blotmentioning

confidence: 99%

“…Recent studies have clarified that the anti-subunit hemagglutinin pili protein 7.9 kDa and subunit hemagglutinin pili protein 49.8 kDa S. dysenteriae are adhesion molecules [9,10]. Subsequent studies have confirmed that there are similar epitopes between subunit pili protein 7.9 kDa S. dysenteriae and subunit pili protein 7.9 kDa S. boydii, and between subunit pili protein 49.8 kDa S. dysenteriae and pili subunit protein 49.8 S. boydii as well, but differences in epitopes than with sub unit pili 7.9 kDa and 49.8 kDa protein in S. flexneri and S. sonnei [11].…”

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confidence: 99%

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Cross Reaction among Antibody Pili sub unit Hemagglutinin Proteins and Outer Membrane sub unit Hemagglutinin Proteins of Shigella flexneri

Fitrianingsih

Raras

Milliana

et al. 2017

JTLS

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Shigella flexneri is the most common causal agent of shigellosis. Its pili are composed of pili protein subunits. Adhesion molecules can be found on the pili and outer membrane proteins (Omp). A hemagglutination reaction can be used for screening of adhesion molecules. Objectives: The purpose of this study was to determine the molecular weight of the pili protein subunits and outer membrane proteins of S. flexneri that act as hemagglutinin proteins, and to prove whether there is a cross-reaction between antibodies against hemagglutinin pili protein subunits and outer membrane proteins of S. flexneri. Methods: Pili protein subunits were isolated using pili bacteria cutters, and the outer membrane proteins were solubilized and obtained using sodium dodecyl sulfate 0.05% as detergent for Omp isolation. The hemagglutination reaction used mice erythrocytes. The cross reactions between subunit pili proteins were conducted by Western blot and Dot blot. Results:. Antibodies against hemagglutinin sub unit pili protein 18 kDa responded to pili protein subunits 18 kDa; 23 kDa; 34 kDa; and 53 kDa; and Omp 23 kDa and 27 kDa. Omp and subunit pili proteins S. flexneri consists of several identical epitopes that were responsible for the similarity of the response profile in the cross-reactions of antibodies.

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Section: Antigen-antibody Reaction By the Methods Of Dot Blotmentioning

confidence: 99%

mentioning

confidence: 99%

Cross Reaction among Antibody Pili sub unit Hemagglutinin Proteins and Outer Membrane sub unit Hemagglutinin Proteins of Shigella flexneri

Fitrianingsih

Raras

Milliana

et al. 2017

JTLS

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Cross Protectivity Analysis of 49.8 kDa Pili Subunits of S. flexneri against Vibrio cholerae Infection

Hidayati

Rully

Amalia

et al. 2022

Interdisciplinary Perspectives on Infectious Diseases

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Background. Although the AMV and AMS vaccine candidates have similar characteristics as hemagglutinin and adhesive molecules, there are differences in molecular weight. Objective. The research aims to determine the immunological cross-reaction between AMS and AMV. Method. Antihemagglutination test used the anti-adhesion molecular antibody AMS. Next, we examined the immune response that has to be linked with protectivity. The model of the research uses MLIL. The sample separated the mice into four groups, and each group had five mice. The first group was the negative control group. The second group was given AMV and infected with Shigella flexneri. The third group was immunized with AMV before being exposed to Shigella flexneri. The last group was infected with Vibrio cholerae. The immune response results were evaluated by calculating the weight of MLIL and counting the colony of bacteria. We also examined other AMS immune responses, namely, β-defensin and s-IgA levels. To get the data, we measured the number of Th17 immune effector cells, T-reg, and proinflammatory cytokine IL-17A. Data analysis was performed using ANOVA, independent t-test, Kruskal–Wallis, and Mann–Whitney tests. Results. An antihemagglutination cross immune response, intestinal weight, the number of bacterial colonies, and other findings were found to be significant ( p < 0.05 ) for the levels of β-defensin, s-IgA, Th17, T-reg, and IL-17A. Conclusion. The 49.8 kDa·MW protein subunit of the Shigella flexneri adhesion molecule could act as a candidate vaccine homologous for shigellosis and cholera in the future.

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Shigella flexneri vaccine development: Oral administration of peptides derived from the 49.8 kDa pili protein subunit activates the intestinal immune response in mice

et al. 2022

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Background and Aim: The morbidity and mortality of Shigella infections remain a global challenge. Epitope-based vaccine development is an emerging strategy to prevent bacterial invasion. This study aimed to identify the ability of the 49.8 kDa pili subunit adhesin protein epitope of Shigella flexneri to induce an intestinal immune response in mice. Materials and Methods: Thirty adult male Balb/c mice were divided into a control group, cholera toxin B subunit (CTB) group, CTB+QSSTGTNSQSDLDS (pep_1) group, CTB+DTTITKAETKTVTKNQVVDTPVTTDAAK (pep_2) group, and CTB+ ATLGATLNRLDFNVNNK (pep_3). We performed immunization by orally administering 50 μg of antigen and 50 μl of adjuvant once a week over 4 weeks. We assessed the cellular immune response by quantifying T helper 2 (Th2) and Th17 using flow cytometry. In addition, we assessed the humoral immune response by quantifying interleukin (IL-4), IL-17, secretory immunoglobulin A (sIgA), and β-defensin using enzyme-linked immunoassay. Statistical analysis was performed using one-way analysis of variance and Kruskal–Wallis test. Results: Peptide oral immunization increases the cellular immune response as reflected by the increase of Th2 (p=0.019) and Th17 (p=0.004) cell counts, particularly in the CTB_pep_1 group. Humoral immune response activation was demonstrated by increased IL-4 levels, especially in the CTB+pep_3 group (p=0.000). The IL-17 level was increased significantly in the CTB+pep_1 group (p=0.042). The mucosal immune response was demonstrated by the sIgA levels increase in the CTB+pep_3 group (p=0.042) and the β-defensin protein levels (p=0.000). Conclusion: All selected peptides activated the cellular and humoral immune responses in the intestine of mice. Further studies are necessary to optimize antigen delivery and evaluate whether the neutralizing properties of these peptides allow them to prevent bacterial infection.

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Haemaglutination of Shigella dysenteriae subunit pili protein with anti-haemaglutination of S. dysenteriae subunit pili protein as a molecule adhesion in mouse enterocytes

Cited by 5 publications

References 23 publications

Cross Reaction among Antibody Pili sub unit Hemagglutinin Proteins and Outer Membrane sub unit Hemagglutinin Proteins of Shigella flexneri

Cross Reaction among Antibody Pili sub unit Hemagglutinin Proteins and Outer Membrane sub unit Hemagglutinin Proteins of Shigella flexneri

Cross Protectivity Analysis of 49.8 kDa Pili Subunits of S. flexneri against Vibrio cholerae Infection

Shigella flexneri vaccine development: Oral administration of peptides derived from the 49.8 kDa pili protein subunit activates the intestinal immune response in mice

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