Haemato-biochemical, mutagenic, and histopathological changes in Oreochromis niloticus exposed to BTX

Sayed, Alaa El‐Din H.; Idriss, Shaimaa K; Abdel-Ghaffar, Sary; Hussein, Ahmed

doi:10.1007/s11356-023-26604-2

Cited by 5 publications

(2 citation statements)

References 76 publications

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“…The histopathological abnormalities in the liver and kidney of exposed groups are in the same line with those reported in the studies of Sayed et al 7 , 8 . As a consequence of loss of antioxidant defensive shield to fight damaging free radicals and increase in death-mediated proteoses 52 , 53 , and following the observations of Sayed et al 7 , 54 , our results revealed presence of necrotic alterations in the liver of benzene group and epithelial sloughing in the kidney of benzene and toluene groups. Loss of hepatocellular integrity is associated with the release of its metabolizing enzymes into the bloodstream 40 .…”

Section: Discussionsupporting

confidence: 90%

“…The quantities of benzene and toluene administered were determined through field studies conducted to investigate residues of commonly used products along the Nile River in four cities within the Assiut governorate 9 . The rationale behind choosing these doses was their capacity to induce disturbances in the redox equilibrium and trigger histopathological alterations in the liver and kidneys Sayed et al 7 . The fourth and fifth groups (SP + benzene and SP + toluene, respectively) were intoxicated with benzene and toluene as previously mentioned following the dietary addition of SP at a dose of 5 g/kg diet for 30 days.…”

Section: Methodsmentioning

confidence: 99%

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Prefeeding of Clarias gariepinus with Spirulina platensis counteracts petroleum hydrocarbons-induced hepato- and nephrotoxicity

Sayed,

Khalil,

Alghriany

et al. 2024

Sci Rep

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Petroleum aromatic hydrocarbons are considered one of the most dangerous aquatic pollutants due to their widespread across water bodies, persistence, and extension to the food chain. To our knowledge, there hasn’t been any research investigating the hepatorenoprotective effects of Spirulina platensis (SP) against toxicity induced by these environmental toxicants in fish. Thus, we decided to explore its potential safeguarding against benzene and toluene exposure in adult Clarias gariepinus. To achieve this objective, fish were divided into five groups (60 per group; 20 per replicate). The first group served as a control. The second and third groups were intoxicated with benzene and toluene at doses of 0.762 and 26.614 ng/L, respectively for 15 days. The fourth and fifth groups (SP + benzene and SP + toluene, respectively) were challenged with benzene and toluene as previously mentioned following dietary inclusion of SP at a dose of 5 g/kg diet for 30 days. The marked increase in liver metabolizing enzymes, glucose, total protein, albumin, globulin, albumin/globulin ratio, and creatinine confirmed the hepato- and nephrotoxic impacts of benzene and toluene. These outcomes were coupled with cytopathological affections and excessive collagen deposition. The incorporation of SP in ration formulation, on the contrary, restored the previously mentioned toxicological profile due to its antioxidant and cytoprotective attributes. Regardless of SP intervention, the renal tissues still displayed histo-architectural lesions, because of insufficient dose and timeframe. Additional research will be required to identify the ideal SP remediation regimen.

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Section: Discussionsupporting

confidence: 90%

Section: Methodsmentioning

confidence: 99%

Prefeeding of Clarias gariepinus with Spirulina platensis counteracts petroleum hydrocarbons-induced hepato- and nephrotoxicity

Sayed,

Khalil,

Alghriany

et al. 2024

Sci Rep

Self Cite

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Strengthening growth, digestion, body composition, haemato-biochemical indices, gene expression, and resistance to Fusarium oxysporum infection and histological structure in Oreochromis niloticus by using fructooligosaccharides and β-1,3 glucan mixture

Abd El-Aziz,

Jaber,

Nass

et al. 2024

Aquacult Int

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Prebiotics are fibers that promote beneficial gut bacteria and play a pivotal role in enhancing host health. This study delves into the impact of various levels of prebiotics, specifically fructooligosaccharides and β-1,3 glucan (Fβ), on the growth performance, biochemical, hematological parameters, gene expression, histological variations in the internal organs, and disease resistance to Fusarium oxysporum in tilapia (Oreochromis niloticus). Two hundred forty Nile tilapia, initially weighing 34.0 ± 0.1 g, were distributed into four groups and given a commercial diet with varying Fβ treatments: control (0 g/kg) (C), T1 (0.5 g/kg), T2 (1.0 g/kg), and T3 (1.5 g/kg) for 70 days. The study revealed significant improvement of the biochemical, hematological, and digestive enzyme activities, as well as histological changes in hepatopancreatic, intestine, and spleen sections with the use of Fβ. Moreover, the expression of innate humoral genes significantly increased (P < 0.05) in tilapia at the 1.5 g/kg Fβ group compared to the control. Notably, challenging with F. oxysporum exhibited lower mortality rates in the three treatments supplemented with additive prebiotics (P < 0.05). Consequently, the feed additives utilized in this study emerge as a viable alternative to enhance growth performance, biochemical and hematological parameters, gene expression, histological variations in internal organs, and disease resistance in fish farming on a large scale.

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Oxidative Stress and Immunopathological Alterations of Clarias gariepinus Exposed to Monocyclic Aromatic Hydrocarbons (BTX)

Sayed

Soliman

Idriss

et al. 2023

Water Air Soil Pollut

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This study’s goal was to assess the catfish’s response to exposure to monoaromatic petroleum hydrocarbons (benzene, toluene, and xylene) and its recovery after exposure using oxidative stress, histopathological, and immunological changes as biomarkers. Four groups: one as control and other three exposed to benzene (0.762 ng/L), toluene (26.614 ng/L), and xylene (89.403 ng/L), respectively, for 30 days and then recovery period for 30 days. The levels of the cortisol, lipid peroxidation, and cytokines (IL-1β, IL-6) increased significantly (p < 0.05) after exposure to benzene and xylene compared to control. Superoxide dismutase (SOD), total antioxidant capacity (TAC), and acetylcholinstease (Ach) decreased significantly (p < 0.05) in fishes exposed to benzene only compared to control group. While glutathione-S-transferase (GST) did not show any change in different treatment groups compared to control group. The histopathological signs of liver exposed to benzene, toluene, and xylene displayed aggregation of melanomacrophages, congestion of sinusoids, vacuolar degeneration of hepatocytes, necrotic area with inflammatory cell infiltration, and thrombus of central vein. Kidney exposed to benzene, toluene, and xylene showed dilatation of Bowman’s space with atrophy of glomerular tuft, lyses of RBCs with mononuclear cell infiltration, multifocal area of hemopoietic tissue necrosis, organized thrombus with perivascular hemorrhage, focal inflammatory cellular reaction, renal tubular necrosis, and thrombus of blood vessels. Spleen exposed to benzene, toluene, and xylene showed hyperplasia of lymphoid follicles in white pulp in a mild degree. These lesions appeared to a mild degree or disappeared completely after recovery period to BTX except spleen. In conclusion, monocyclic aromatic hydrocarbons (BTX) are hazardous to fish and the toxicity level was as benzene > xylene > toluene even though after recovery period.

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Haemato-biochemical, mutagenic, and histopathological changes in Oreochromis niloticus exposed to BTX

Cited by 5 publications

References 76 publications

Prefeeding of Clarias gariepinus with Spirulina platensis counteracts petroleum hydrocarbons-induced hepato- and nephrotoxicity

Prefeeding of Clarias gariepinus with Spirulina platensis counteracts petroleum hydrocarbons-induced hepato- and nephrotoxicity

Strengthening growth, digestion, body composition, haemato-biochemical indices, gene expression, and resistance to Fusarium oxysporum infection and histological structure in Oreochromis niloticus by using fructooligosaccharides and β-1,3 glucan mixture

Oxidative Stress and Immunopathological Alterations of Clarias gariepinus Exposed to Monocyclic Aromatic Hydrocarbons (BTX)

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