Haematocrit corrected analysis of creatinine in dried blood spots through potassium measurement

Burger, Jeroen C. G. den; Wilhelm, Abraham J.; Chahbouni, Abdel; Vos, R.M.; Sinjewel, A.; Swart, Eleonora L.

doi:10.1007/s00216-014-8291-9

Cited by 27 publications

(12 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In essence, because of Hct-dependent spreading of blood on filter paper (blood with higher Hct spreading less), partial punch analysis of a DBS (which is the approach mostly used) will most often yield a bias for DBS generated from blood with divergent (either low or high) Hct. However, several strategies have been developed that allow to cope with the issues coupled to a varying Hct [14][15][16][17][18][19][20][21][22]. One of the proposed approaches is the use of volumetric absorptive microsampling (VAMS) devices [23,24].…”

Section: Introductionmentioning

confidence: 99%

Volumetric absorptive microsampling as an alternative tool for therapeutic drug monitoring of first-generation anti-epileptic drugs

Velghe

Stove

2018

Anal Bioanal Chem

View full text Add to dashboard Cite

Dosage adjustment of anti-epileptic drugs by therapeutic drug monitoring (TDM) is very useful, especially for the first-generation anti-epileptic drugs (AEDs). Microsampling-the collection of small volumes of blood-is increasingly considered a valuable alternative to conventional venous sampling for TDM. Volumetric absorptive microsampling (VAMS) allows accurate and precise collection of a fixed volume of blood, eliminating the volumetric blood hematocrit bias coupled to conventional dried blood spot collection. The aim of this study was to develop and validate an LC-MS/MS method for the determination and quantification of four anti-epileptic drugs (carbamazepine, valproic acid, phenobarbital, and phenytoin) and one active metabolite (carbamazepine-10,11-epoxide) in samples collected by VAMS. The method was fully validated based on international guidelines. Precision (%RSD) was below 10%, while, with a single exception, accuracy (%bias) met the acceptance criteria. Neither carry-over nor unacceptable interferences were observed, the method being able to distinguish between the isomers oxcarbazepine and carbamazepine-10,11-epoxide. All compounds were stable in VAMS samples for at least 1 month when stored at room temperature, 4 °C, and - 20 °C and for at least 1 week when stored at 60 °C. Internal standard-corrected matrix effects were below 10%, with %RSDs below 4%. High (> 85%) recovery values were obtained and the effect of the hematocrit on the recovery was overall limited. Successful application on external quality control materials and on left-over patient samples demonstrated the validity and applicability of the developed procedure. Graphical abstract Graphical representation of the sampling, chemical structures, and the resulting chromatogram for volumetric absorptive microsampling (VAMS)-based therapeutic drug monitoring of first-generation anti-epileptic drugs by liquid chromatography with tandem mass spectrometric detection.

show abstract

Section: Introductionmentioning

confidence: 99%

Volumetric absorptive microsampling as an alternative tool for therapeutic drug monitoring of first-generation anti-epileptic drugs

Velghe

Stove

2018

Anal Bioanal Chem

View full text Add to dashboard Cite

show abstract

“…Potassium measurements from stored DBS and in stored extracts were found to remain stable for several months even at room temperature (Capiau et al, 2013; den Burger et al 2015). In addition, no correlation was observed between K + measurements and ‘age of spot’ (Pearson correlation coefficient −0.085), indicating that potassium measurements were not associated with storage conditions.…”

Section: Resultsmentioning

confidence: 99%

An untargeted metabolomics method for archived newborn dried blood spots in epidemiologic studies

et al. 2017

View full text Add to dashboard Cite

Introduction For pediatric diseases like childhood leukemia, a short latency period points to in-utero exposures as potentially important risk factors. Untargeted metabolomics of small molecules in archived newborn dried blood spots (DBS) offers an avenue for discovering early-life exposures that contribute to disease risks. Objectives The purpose of this study was to develop a quantitative method for untargeted analysis of archived newborn DBS for use in an epidemiological study (California Childhood Leukemia Study, CCLS). Methods Using experimental DBS from the blood of an adult volunteer, we optimized extraction of small molecules and integrated measurement of potassium as a proxy for blood hematocrit. We then applied this extraction method to 4.7-mm punches from 106 control DBS samples from the CCLS. Sample extracts were analyzed with liquid chromatography high resolution mass spectrometry (LC-HRMS) and an untargeted workflow was used to screen for metabolites that discriminate population characteristics such as sex, ethnicity, and birth weight. Results Thousands of small molecules were measured in extracts of archived DBS. Normalizing for potassium levels removed variability related to varying hematocrit across DBS punches. Of the roughly 1,000 prevalent small molecules that were tested, multivariate linear regression detected significant associations with ethnicity (3 metabolites) and birth weight (15 metabolites) after adjusting for multiple testing. Conclusions This untargeted workflow can be used for analysis of small molecules in archived DBS to discover novel biomarkers, to provide insights into the initiation and progression of diseases, and to provide guidance for disease prevention.

show abstract

“…While this technique is not sensitive enough for trace elemental analysis, and still requires matrix-matched solid standards for calibration, it is completely nondestructive, does offer information about the surface of the whole spot, minimizing distribution issues, and can provide complementary information for elements present at high levels that are also of clinical interest. In this regard, the importance of the determination of K can be highlighted, because it has been shown that its content can be used to correct for hematocrit variations [58,71,72].…”

Section: Direct Analysis Of Dmsmentioning

confidence: 99%

Dried matrix spots and clinical elemental analysis. Current status, difficulties, and opportunities

Resano

Belarra

García-Ruiz

et al. 2018

TrAC Trends in Analytical Chemistry

View full text Add to dashboard Cite

This article examines the increasing importance of dried matrix spot (DMS) specimens (such as dried blood spots, dried urine spots, etc.) in biomedical research, the challenges associated with their analysis when quantitative elemental information is aimed at, as well as the benefits deriving from the further usage of these types of samples. The article briefly reviews the historical evolution of this sampling approach in elemental clinical analysis, stressing prospective areas of applications (e.g., newborns or prosthesis control), the methodologies most recently developed to produce DMS of known volume, as well as novel strategies proposed to analyze them, often related to direct solid sampling techniques or fast lixiviation methods. Finally, the article discusses the type of information that could be obtained after isotopic analysis of DMS when targeting non-traditional stable isotopes (e.g., Cu, Fe or Zn), which can significantly help in the early diagnosis of some medical conditions (e.g. Wilson's disease).

show abstract

Haematocrit corrected analysis of creatinine in dried blood spots through potassium measurement

Cited by 27 publications

References 4 publications

Volumetric absorptive microsampling as an alternative tool for therapeutic drug monitoring of first-generation anti-epileptic drugs

Volumetric absorptive microsampling as an alternative tool for therapeutic drug monitoring of first-generation anti-epileptic drugs

An untargeted metabolomics method for archived newborn dried blood spots in epidemiologic studies

Dried matrix spots and clinical elemental analysis. Current status, difficulties, and opportunities

Contact Info

Product

Resources

About