Haematological and Biochemical Profile of Wistar Rats Exposed to Chloroform Stem Extract of Portulaca oleracea Linn. (Purslane)

Abstract

Portulaca oleracea Linn. has a history of extensive use as a medicinal plant and is a component of the Nigerian ethnomedical pharmacopoeia. Although several studies have been carried out exclusively on the leaves to ascertain the physiological effect, there seems to be dearth of literature on the physiological effect of the stem. This study which was designed to fill this gap investigated the sub-chronic effect of oral administration of chloroform stem extract of P.oleracea (CSEPO) on haematological parameters and plasma activities of some enzymes (Alanine Aminotransferase - ALT, Alkaline Phosphatase - ALP & Aspartate Aminotransferase - AST) and levels of other biochemical parameters such as total protein, albumin, bilirubin, urea and creatinine in male wistar rats. Twenty animals were randomly divided into 4 groups of 5 rats each. Group A(Control) received 0.5 ml of olive oil(vehicle) while Groups B, C & D received 125, 250 & 500 mg/kg body weight of CSEPO respectively for 21 days by oral gavage. At the end, the animals were anaesthetized and blood samples were collected for haematology and plasma biochemical assay. No significant (P > .05) variation occurred in the mean values of ALT, ALP, AST, total protein, albumin, bilirubin, urea, creatinine, Packed Cell Volume (PCV), haemoglobin concentration, RBC, WBC and differential leucocyte counts relative to the control. There was a highly significant (P < .01) increase in the total platelet count only at the dose of 500mg/kg in comparison with control. Oral administration of CSEPO as used in this study had no adverse effect on haematological and biochemical parameters. Secondly, CSEPO may be a useful remedy in thrombocytopenia, due to its potential to increase platelet count. However, further study is recommended in that regards.

Beneficial Effects of Resveratrol and γ-Cyclodextrin on the Hematological and Biochemical Parameters of Healthy Wistar Rats Treated with Cisplatin: A PCA Approach

“…The same observations resulted from other similar studies on mice and rats, where no significant changes in the body weight and hematological parameter values were observed after the administration of CP-based formulations at a 5 mg/kg body weight and after 6 days [11,12,27]. LY% was slightly higher than the normal limits in rats; however, the total LYM count was in the normal range [43][44][45][46]. No lymphocytopenia (low level of LYM) was observed after CP or CP-based formulation treatments.…”

“…A decrease in the iron-related hematological parameters was observed for the γ-CD/CP-treated group. The RBC and HGB values were only 7.1 × 10 12 /L and 12.3 g/dL (slightly lower than the normal values of 5.3-10 × 10 12 /L and 14-18 g/dL, respectively) [43][44][45][46], whereas for the γ-CD/CP binary and γ-CD/Rv/CP ternary complexes they were 7.9-8.4 × 10 12 /L and 13.7-14.7 g/dL, respectively (lower values for the binary complex). These differences occurred in healthy rats due to some kidney problems that were more affected by the treatment with the CP solution (anemia), less affected after treatment with the γ-CD/CP binary complex, and almost not affected by using the antioxidant-protected γ-CD/Rv/CP ternary complex.…”

“…However, the differences were minor. The HCT parameter also had lower values after the γ-CD/CP binary complex treatment, which were at the limit of the normal values in healthy rats (35-52%) [43][44][45][46]. The difference was in accordance with the correlation of HCT with the RBC and HGB parameters.…”

Curcumin mitigates acrylamide‐induced ovarian antioxidant disruption and apoptosis in female Balb/c mice: A comprehensive study on gene and protein expressions

“…The cytotoxic impact of ACR may trigger ROS formation, leading to cytotoxic and genotoxic consequences through alterations in the cellular redox state (Gao et al., 2023 ). Research has shown that the bioactive constituents present in certain plants possess the capacity to alleviate oxidative stress (Alaee et al., 2023 ; Bolouki et al., 2020 ; Mbiba Hassanu et al., 2022 ; Mohammadi et al., 2023 ; Obinna & Agu, 2023 ; Ojatula, 2021 ; Oyetunji et al., 2021 ; Yaghutian Nezhad et al., 2021 ). Hence, it appears that antioxidant molecules might aid in mitigating the deleterious effects of this compound.…”

The antioxidant properties of resveratrol on sperm parameters, testicular tissue, antioxidant capacity, and lipid peroxidation in isoflurane-induced toxicity in mice

“…13 Many herbal plants contain antioxidant properties which can preserve reproductive tissues against different toxicity. [14][15][16][17][18][19][20] Resveratrol (also known as 3,5,4'-trihydroxy-trans-stilbene) is a natural phytoalexin. 21,22 There are at least 27 plant species that contain this compound.…”