Haematological and Biochemical Reference Values for Healthy Population of Maferinyah Rural Community in Guinea

Abstract

Guinea’s reference ranges for biological parameters rely on those of Caucasian values. Variability in reference ranges according to the context is well-documented. We conducted this study for the purpose of future malaria clinical trials that assess the efficacy and safety of malaria drugs. A repeated cross-sectional study was carried out, in an apparently healthy cohort population. Surveys took place in Maferinyah rural community, which is located at 75 km from the capital. The 2.5th and 97.5th percentiles were determined nonparametrically and stood for reference intervals. Reference values were determined separately for males and females according to ranges of age (6-10 years of age; 11-15 years of age; 16-45 years of age). Differences between genders were tested using the Mann-Whitney test, while the Friedman test was performed to test differences within each gender group according to the seasons. A total of 450 volunteers were enrolled. The median age was 13. Males 16-45 years of age had significantly higher hematologic and biochemical values compared to a female of the same age (for hematological parameters: Mean Cell Hemoglobin Concentration MCHC p≤0.001, Platelets p≤0.001, monocytes p=0.0305, eosinophils p=0.0225; for biochemical parameters: Aspartate aminotransferase AST p≤0.001, Alanine Aminotransferase ALT p≤0.001, creatinine p≤0.001). We noticed significant seasonal variations for all the biochemical parameters and some hematologic parameters (Mean Corpuscular Hemoglobin MCH, MCHC, Mean Cell volume). This is the first study establishing hematologic and biochemical parameters in Guinea. These findings provide a useful guide for the clinical researchers and care providers. Studies on large scale and in different settings would be also desirable.

Establishment of Hematological Reference intervals for healthy Children in Elobied City, Sudan

“…several studies revealed significant differences in hematology reference range amongst children of different ages, as well as in populations, seasons, racialethnic groups, and gender subgroups. (Sahoo et al,2015, Béavogui et al 2020. Current findings revealed that the mean of Hb concentration regarding different age groups ( 3-7, 8-12, 13-17) is (12.4, 12, 12.5, 12.4) respectively, this agrees with Al-Jafars' study (Al-Jafar, 2016).…”

Complete Blood Count Reference Intervals for Children Aged Less Than 1 to 12 Years in the Northern Region of Ghana

“…To discourage this trend, this study is aimed at creating a local standard for interpreting medical laboratory findings by determining the CBC reference intervals for children aged less than 1-12 years in Tamale, Northern Ghana. This study observed no significant differences in most CBC reference intervals when compared by sex and age group, except for Hb, MCV, MCH, MCHC, PLT, and absolute basophils, similar to studies in Guinea [21] and China [22], but contrary to a study in Macedonia [23], which showed a steady increase in CBC reference intervals with age. The inability of this study to detect and exclude children who may present with mild anaemia due to thalassaemia, evaluate their nutritional status to rule out conditions such as subclinical iron deficiency anaemia, and instead rely solely on subjective health and the absence of persistent or recent diseases may account for this difference in outcomes [21,22,24].…”

“…This study observed no significant differences in most CBC reference intervals when compared by sex and age group, except for Hb, MCV, MCH, MCHC, PLT, and absolute basophils, similar to studies in Guinea [21] and China [22], but contrary to a study in Macedonia [23], which showed a steady increase in CBC reference intervals with age. The inability of this study to detect and exclude children who may present with mild anaemia due to thalassaemia, evaluate their nutritional status to rule out conditions such as subclinical iron deficiency anaemia, and instead rely solely on subjective health and the absence of persistent or recent diseases may account for this difference in outcomes [21,22,24]. This was confirmed by the statistically significant differences in Hb, MCV, MCH, and MCHC by sex and age group observed in this study [23,25].…”

Prediction of plasma volume and total hemoglobin mass with machine learning

“…Because PV changes are not considered in the [Hb] thresholds established by the WHO to diagnose anemia, this diagnostic model presents substantial limitations (16,17). Numerous studies have soundly measured reference hematological values, including [Hb], in populations with high anemia prevalence according to the WHO website, notably in women of reproductive age and young children (< 5 years old) (39)(40)(41)(42)(43)(44)(45)(46). Such countries are described with a severe anemia prevalence by the WHO with values going from 40% to 69% in either populations (12,47).…”