Haematological and Blood Chemical Values from Bothrops ammodytoides (Ophidia–Crotalidae) in Captivity

Troiano, Juan Carlos; Vidal, Juan C.; Gould, E.; Malinskas, G.; Gould, J. A.; Scaglione, M.; Scaglione, L.; Heker, J.; Simoncini, C.; Dinápoli, H.

doi:10.1007/pl00010004

Cited by 9 publications

(17 citation statements)

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“…Em outros viperídeos, como B. ammodytoides estudados por Troiano et al (1999), os valores foram semelhantes. B. alternatus teve valores menores de colesterol e semelhantes de triglicéri-des (Troiano et al 1998).…”

Section: Methodsunclassified

Determinação das concentrações plasmáticas de proteínas e metabólitos de cascavéis em cativeiro

et al. 2014

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Resumo: Para determinar as concentrações plasmáticas de proteínas e metabólitos de cascavéis em cativeiro, foram utilizadas 60 serpentes adultas, sendo 30 machos e 30 fêmeas. O sangue foi coletado através de punção do seio venoso paravertebral cervical e armazenado em tubos com heparina. As análises bioquímicas foram processadas colorimetricamente em Analisador Automático de Bioquímica Chemwell (Awareness Technology®, Inc). Foram calculadas as médias e desvios padrão dos seguintes constituintes: proteínas totais, albumina, globulinas, relação albumina/globulinas, ácido úrico, creatinina, ureia, colesterol, colesterol HDL e triglicérides. Os valores obtidos foram semelhantes aos descritos na literatura para repteis e serpentes, sendo as diferenças observadas provavelmente decorrentes da diferença entre espécies, clima, estação do ano e metodologia utilizada. Não houve diferenças significativas entre machos e fêmeas para os parâmetros estudados. Estes resultados podem ser úteis no estabelecimento de valores de referência para planos de conservação destes ofídios em cativeiro.

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Section: Methodsunclassified

Determinação das concentrações plasmáticas de proteínas e metabólitos de cascavéis em cativeiro

et al. 2014

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“…One hundred cells were counted in each slide. The leukocyte types are described according to the morphological nomenclature proposed by (Hawkey and Dennet, 1994) in which the percentage of every white cell recorded including; Neutrophile, Eosinophile, Basophile, Lymphocyte, and Monocyte (Troiano et al, 1999).…”

Section: Peripheralmentioning

confidence: 99%

Relationship between C- Reactive Protein, Differential Leukocyte Count and Uric Acid in Kidney Disease in Erbil Teaching Hospital.

2017

ZJPAS

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The study purpose was to evaluate the relationship between CRP, differential leukocyte count and uric acid in people with and without chronic kidney disease (CKD). A case-control study carried out at kidney disease unite, Erbil teaching hospital, from June 2013 till November 2013. Patient with diagnosed CKD being included with regular follow-up with no evidence of infection at time of interview by history, clinical examination and investigations including renal function testing, serum uric acid, CRP, general urine examination and other relevant investigations .The control group in general population were apparently healthy evaluated by the same way. There was significant reduction (p<0.001) in lymphocyte level (12.52± 0.94) compared to control group (26.0±0.84) and significant increase was observed for CRP (10.56±0.61 ng/mL) in CKD patients compared with the control group (3.36±0.69 ng/mL) at the level (p<0.001).Serum uric acid was significantly high in patients with CKD (7.05±0.205 mg/mL) when compared to controls (4.3±0.27 mg/mL) at the level (p< 0.001).Lymphocyte count showed significant reduction (p<0.001) (12.93± 0.86) and (13.46± 0.84) compared to control group (25.2±1.45) and (26.35± 0.7) respectively, while other differential leukocyte count show no significant difference when compared with control group. CRP negatively and significantly correlated with lymphocyte level (-0.626), while serum CRP level positively correlated with neutrophil level (0.547) and monocyte level (0.291). Also serum uric acid negatively and significantly correlated with lymphocyte level (-0.530), and positively correlated with neutrophil level (0.450) and monocyte level (0.236). Compared with those without CKD, patients with CKD have more eosinophil and granulocytes, CRP, uric acid and fewer lymphocytes. Hyperuricemia is a valuable predictor of a GFR decrease, early detection and prevention on hyperuricemia in CKD subjects are critical.

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“…Frye (1978) gave a description of cells obtained from the vertebral column and also in his Atlas of Reptilian Care (intended for veterinarians) (Frye and Frye 1991) described a method of obtaining marrow tissue from large and smaller snakes, but without a detailed description of that tissue. Until now the majority of researchers, starting with Mandl (1839) and Gulliver (1840Gulliver ( , 1875, have only occasionally been interested in the quantitative studies on the peripheral blood cells of reptilian species, including snakes (Bergman 1957;Hutton 1958;Saint-Giron 1961;Binyon and Twigg 1965;Saint-Giron and Saint-Giron 1969;Wirth 1972;Otis 1973;McMahon and Hamer 1975;Board et al 1977;Al-Badry and Nuzhy 1983;Pati and Thapliyal 1984;Troiano et al 1997Troiano et al , 1999, or in describing the morphology of various blood cells at both the light and electron microscopy level (Kélenyi and Németh 1969;Montali 1988;Sano-Martins et al 1994;SpadacciMorena et al 1998;Alleman et al 1999). Excellent work on that subject has been done by Pienaar (1962), Sypek and Borysenko (1988) and Frye and Frye (1991).…”

Section: Introductionmentioning

confidence: 99%

Haematopoiesis and a new mechanism for the release of mature blood cells from the bone marrow into the circulation in snakes (Ophidia)

Sano-Martins

Dabrowski

Tabarowski

et al. 2002

Cell and Tissue Research

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This is the first description of haematopoiesis in snakes. Studies were carried out on the following species belonging to Ophidia: Bothrops jararaca, Bothrops jararacusu, Waglerophis merremii, Elaphe taeniura taeniura, Boa constrictor,and Python reticulatus. Smears of the peripheral blood and histological preparations from the vertebrae, ribs, liver, and spleen were studied under a light and electron microscope. Myeloid cells were present in the following locations in the vertebrae: the neural spine, zygoapophysial processes, floor of the neural canal, lacunae in the bodies of vertebrae and also inside the ribs. Although the vascular system was well developed, especially around the ribs, vessels inside the marrow cavities were scarce, both in the ribs and elsewhere where haematopoiesis was found. Venous sinuses were well developed in the vertebrae and in the rib regions from their costal head towards the middle area. They consisted of one layer of fine endothelial cells. Mature cells in the process of migration into the general circulation were only sporadically encountered when venous sinuses were studied on perfusion-fixed specimens. In contrast, almost every sinus venosus contained protrusions directed towards the lumen, filled mostly with mature and immature blood cells. Various stages of their formation were seen in the cross sections of venous sinuses ranging from small, newly formed to large, elongated ones, filled with many fully developed and some maturing blood cells. In many cases the apices of the protrusions were ruptured, and mature blood cells, as well as a few immature ones, were seen in their vicinity. This observation led us to a new hypothesis that blood cells are released from the extravascular space into the lumen of venous sinuses. In snakes, these cells are released into the systemic circulation mainly via the rupture of protrusions filled with mature blood cells and, to a lesser degree, by transcytosis as known in mammals. In the spleens from young specimens, 1-2 foci of haematopoiesis were encountered where lymphopoiesis predominated. Haematopoiesis was not detected in the liver.

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Haematological and Blood Chemical Values from Bothrops ammodytoides (Ophidia–Crotalidae) in Captivity

Cited by 9 publications

References 0 publications

Determinação das concentrações plasmáticas de proteínas e metabólitos de cascavéis em cativeiro

Determinação das concentrações plasmáticas de proteínas e metabólitos de cascavéis em cativeiro

Relationship between C- Reactive Protein, Differential Leukocyte Count and Uric Acid in Kidney Disease in Erbil Teaching Hospital.

Haematopoiesis and a new mechanism for the release of mature blood cells from the bone marrow into the circulation in snakes (Ophidia)

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