Haematological Changes in Trypanosoma brucei brucei Infected Wistar Rats Treated with a Flavonoid Mixture and/or Diminazene Aceturate

Abstract

The aim of the study was to evaluate the effect of treatment with DAFLON® 500 mg (DF) and/or diminazene aceturate (DZ) on hematological parameters in rats, infected with Trypanosoma brucei brucei. Rats in the control group were administered with distilled water (DW) only (5 mL/kg), while those in other groups were infected with Trypanosoma brucei brucei (10 6 cells/ml), and treated with DF and/or DZ. Packed cell volume (PCV), haemoglobin (Hb) concentration, erythrocyte (RBC) and neutrophil counts were lower in the infected untreated and DF-treated group, than in any other group. Total leucocyte, lymphocyte, platelet counts and mean corpuscular volume (MCV) decreased in the infected untreated group, compared to values obtained in those administered with DF and/or DZ. The MCV reduced in the DZ-treated group, compared to the groups treated with DF and the combination of DF and DZ. Mean corpuscular haemoglobin concentration (MCHC) was lower in the infected untreated and DF treated group than in the DW or DZ group. Rats administered with DZ had higher leucocyte and lymphocyte counts compared to those in DF-treated group. It is concluded that the administration of DF and/or DZ ameliorated the anaemia caused by Trypanosoma brucei brucei -infection in Wistar rats.

Comparative assessment of the effect of Diminazene aceturate and Imidocarb dipropionate on haematology and serum biochemical parameters of apparently healthy Nigerian dogs.

“…From our study, the administration of DIAC and IMD did not adversely alter the haematological and serum biochemical profiles of Nigerian local dogs This may refer to the binding of bout 70% of diminazene to red blood cell as well as purified haemoglobin [20], with the absence of any records on its toxic effect on red blood cell, serum and plasma enzymes. In agreement with our results, diminazene aceturate ameliorated the anaemia caused by Trypanosomal brucei brucei infection in rats [21], has the capacity to improve blood components in Trypanosomes infected rats [22], and caused a generalized increase in WBC of dogs treated with various doses of diminazene aceturate [23]. You can write the authors names only before their reference number Bashir et al [24] reported that the administration of Imidocarb caused no significant changes in complete blood count (CBC), bilirubin concentration and (BUN) between pre-and post-treatment periods in dogs.…”

Ginkgo biloba attenuated detrimental inflammatory and oxidative events due to Trypanosoma brucei rhodesiense in mice treated with melarsoprol

“…These include blood components, resulting in anaemia [ 43 ]. Erythrocyte cell damage occurs in the course of HAT because of overwhelming oxidative stress [ 44 ]. This study confirmed the presence of trypanosomiasis-mediated anaemia, marked by low counts of RBC, HCT and haemoglobin.…”