Haemocompatibility assessment of synthesised platinum nanoparticles and its implication in biology

Abstract

The growing need for advanced treatment of evolving diseases has become a motivation for this study. Among the noble metals, platinum nanoparticles are of importance because of their catalytic property, antioxidant potential, minimal toxicity and diverse applications. Biological process of synthesis has retained its significance, because it is a simple one-step process yielding stable nanoparticles. Herein, we have synthesised platinum nanoparticles through a green process using the unexplored seaweed Padina gymnospora, a brown alga. The course of synthesis was monitored and the nanoparticles were characterised using UV-visible spectroscopy. The synthesised nanoparticles were studied using TEM, XRD and FTIR. The TEM and XRD studies reveal the size of the nanoparticle to be <35 nm. The catalytic nanoparticles were capable of oxidising NADH to NAD(+). The biocompatibility was tested by haemolytic assay for the furtherance of the application of platinum nanoparticles in medicine. This is the first report on the biogenic synthesis of platinum nanoparticles using seaweed.

Anisotropic Platinum Nanoparticle-Induced Cytotoxicity, Apoptosis, Inflammatory Response, and Transcriptomic and Molecular Pathways in Human Acute Monocytic Leukemia Cells

“…The amount of GSH was significantly decreased to 90, 80, 70, 50, and 20 µM, respectively, in THP-1 cells incubated with 25, 50, 75, 100, 125, and 150 µg/mL PtNPs. These findings agreed with those of previous studies showing decreased GSH concentrations in HT29, A549 lung carcinoma, osteosarcoma, prostate cancer, and THP-1 cells incubated with PtNPs [17,22,27,47].…”

“…Figure 4A shows more LDH leakage at 75-150 µ g/mL PtNPs, indicating a significant decrease in cell viability and a significant increase in LDH release. These findings indicated that the decreased cell viability and damage caused by the NPs ultimately caused the death of the cells [47]. Others also reported that PtNPs induce cytotoxicity through LDH leakage in cancer cells including A549 lung carcinoma, LNCaP prostate cancer, and OS epithelial (U2OS) cells [17,18,47].…”

“…An imbalanced redox potential might also be a cause of PtNP toxicity. Administering PtNPs induces intracellular oxidative stress via the generation of ROS that disrupt the antioxidant system [18,47]. An imbalance in antioxidant enzymes also causes lipid peroxidation [49], which deteriorates the cell membrane and renders it susceptible to further oxidation by free radicals [49,50].…”

Comparative assessment of the phytotoxicity of silver and platinum nanoparticles

“…The concept of biosynthesis is to develop an environment friendly method which was done in this study by using a seaweed Padina gymnospora. Therefore biosynthesis of nanoparticle has been considered as an alternative for synthesizing nanoparticles which has been reported [9], [10]. The seaweed P. gymnospora rich in various bioactive components effectively reduced the salts to form nanoparticles.…”

Interaction of hierarchical nanoporous carbons(HNCs) with chicken embryo red blood cells (RBC)

“…The absorbance of the supernatant, which includes plasma and lysed erythrocytes, was measured at 540 nm (Infinite M200, Tecan, Durham, NC, USA). Percent hemolysis was determined as compared to Shiny et al (2014).…”