Haemocyte Morphology and Differential Haemocyte Counts of Giant Ladybird Beetle, &lt;i&gt;Anisolemnia dilatata&lt;/i&gt; (F.) (Coleoptera:Coccinellidae):A Unique Predator of Bamboo Woolly Aphids

Majumder, Joydeb; Ghosh, Debankita; Agarwala, B. K.

doi:10.18520/cs/v112/i01/160-164

Cited by 2 publications

(1 citation statement)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most studies of hemolymph have been carried out on representatives of the families Lepidoptera, Diptera, Orthoptera, Hymenoptera and Hemiptera [20,21,22,23]. Several studies were also carried out on representatives of the Coccinellidae family: Anisolemnia dilatata (F) [24], Harmonia axyridis [25], and Coccinella septempunctata (L) [26]. In our study, the predominant type of hemocytes was prohemocytes (more than 60%), while in other studied species of the family Coccinellidae, leading a predatory lifestyle, plasmocytes predominated.…”

Section: Resultsmentioning

confidence: 99%

Ontogenetic features of the morphology of hemolymph cells in Henosepilachna vigintioctomaculata (Coleoptera: Coccinellidae) as an indicator of biodiversity

Ермак¹,

Мацишина²,

Фисенко³

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

View full text Add to dashboard Cite

Insect hemocytes are a complex of different types of cells that differ morphologically and functionally. The study of morphological variability of hemocytes Henosepilachna vigintioctomaculata (Coleoptera: Coccinellidae) was carried out, eight types of cells were identified: spherulocytes, enocytoids, adipohemocytes, prohemocytes, plasma cells, granulocytes, cystocytes and phagocytes. The predominant type of hemocytes at all stages of ontogenesis are prohemocytes. In the hemolymph of larvae, the most numerous are prohemocytes with a basophilic nucleus (32.07%), prohemocytes with an eosinophilic nucleus (38.12%), and plasmatocytes (19.4%). The pupa is characterized by prohemocytes (64.3%), the sizes of which coincided with those of the adults (10.09 ± 2.11 and 9.45 ± 1.85 µm). At the adult stage, adipohemocytes, enocytoids and cystocytes were found, which were not observed at the larval stage. The size of hemocytes changed depending on the stage of ontogenesis. Thus, it was found that the cellular composition of the Henosepilachna vigintioctomaculata hemolymph is the same for the adult and pupal stages, but it has differences in the dimensional characteristics of the formed elements. The larval stage has its own characteristics in the cellular composition of the hemolymph: the absence of three types of hemocytes and the formation of two subtypes of prohemocytes.

show abstract

Section: Resultsmentioning

confidence: 99%

Ontogenetic features of the morphology of hemolymph cells in Henosepilachna vigintioctomaculata (Coleoptera: Coccinellidae) as an indicator of biodiversity

Ермак¹,

Мацишина²,

Фисенко³

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

View full text Add to dashboard Cite

show abstract

Chlorantraniliprole Enhances Cellular Immunity in Larvae of Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae)

Liu,

Deng,

Wang

et al. 2024

Insects

View full text Add to dashboard Cite

The innate immunity of insects encompasses cellular and humoral defense mechanisms and constitutes the primary defense against invading microbial pathogens. Cellular immunity (phagocytosis, nodulation, and encapsulation) is primarily mediated by hemocytes. Plasmatocytes and granulocytes play an important role and require changes in the cytoskeletons of hemocytes. However, research investigating the immunological impacts of insecticides on the fall armyworm (FAW), Spodoptera frugiperda, remains scarce. Therefore, we conducted a study to investigate the effects of chlorantraniliprole exposure on cellular immunity in FAW larvae. Our findings revealed the presence of five types of hemocytes in the larvae: prohemocytes, plasmatocytes, granulocytes, oenocytoids, and spherulocytes. The LD10, LD20, and LD30 of chlorantraniliprole affected both the morphology and total count of some hemocytes in the larvae. Moreover, larvae exposed to chlorantraniliprole showed increased phagocytosis, nodulation, and encapsulation. To determine the mechanism of the enhanced cellular immunity, we studied plasmatocytes in the spread state and the cytoskeleton in hemocytes. It was found that the spreading ratio of plasmatocytes and the areas of the cytoskeletons in hemocytes were increased after chlorantraniliprole treatment. These results suggest that exposure to chlorantraniliprole results in an enhanced immune response function in FAW larvae, which may be mediated by cytoskeletal changes and plasmatocyte spreading. Consequently, this study provides valuable insights into the cellular immune response of FAW larvae to insecticide exposure.

show abstract

Haemocyte Morphology and Differential Haemocyte Counts of Giant Ladybird Beetle, <i>Anisolemnia dilatata</i> (F.) (Coleoptera:Coccinellidae):A Unique Predator of Bamboo Woolly Aphids

Cited by 2 publications

References 19 publications

Ontogenetic features of the morphology of hemolymph cells in Henosepilachna vigintioctomaculata (Coleoptera: Coccinellidae) as an indicator of biodiversity

Ontogenetic features of the morphology of hemolymph cells in Henosepilachna vigintioctomaculata (Coleoptera: Coccinellidae) as an indicator of biodiversity

Chlorantraniliprole Enhances Cellular Immunity in Larvae of Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae)

Contact Info

Product

Resources

About