Haemoglobin, a new major allergen of Anisakis simplex

González-Fernández, Juan; Daschner, Álvaro; Nieuwenhuizen, Natalie E.; Lopata, Andreas L.; Frutos, C. De; Valls, A.; Cuéllar, C.

doi:10.1016/j.ijpara.2015.01.002

Cited by 33 publications

(22 citation statements)

References 45 publications

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“…On the contrary, Ani s 1‐ like and Ani s 7‐ like in the present study appear to be the major ES antigens of A. pegreffii , having no homology with other antigens/allergens to which humans are known to be sensitized . As Ani s 13‐ like has also been recognized in the present study in a large number of patients, and cross‐reaction with other nematode nemoglobins has been not documented, this allergen could be also considered as a potential candidate for the specific diagnosis of allergic anisakiasis caused by A. pegreffii . Thus, the three antigens/allergens reported here are those most specific and sensitive for serodiagnostic tests of human anisakiasis .…”

Section: Discussioncontrasting

confidence: 64%

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IgE sensitization to Anisakis pegreffii in Italy: Comparison of two methods for the diagnosis of allergic anisakiasis

Mattiucci

Colantoni

Crisafi

et al. 2017

Parasite Immunology

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IgE sensitization to Anisakis pegreffii in Italian subjects suffering from gastro-allergic anisakiasis (GAA) (N=5), or showing chronic urticaria (CU+) after fish consumption (N=100), was investigated. A control group (N=5) was also included. IgE response was analysed by immunoblotting (WB) assay, using both excretory/secretory products (ESPs) and crude extract (CE) of A. pegreffii larvae. The results were compared with those achieved by the conventional immunological method for Anisakis allergy (ie, immunoCAP). Among the 110 subjects, 28 showed IgE positivity with both WB and iCAP methods; 13 proved IgE reactivity, in WB assay, to ESP antigens of A. pegreffii, here provisionally indicated as Ani s 1-like, Ani s 7-like, Ani s 13-like; only 15 sera have shown IgE-WB reaction to Ani s 7-like and Ani s 13-like. iCAP and WB exhibited a high concordance value (κ=1.00) when iCAP value was <0.35 (negative result) and >50.0 (positive result). In the sera samples recorded as positive to Anisakis allergy, Ani s 1-like was responsible for 46.4% of the sensitivity, while Ani s 7-like and Ani s 13-like for 100%. They could be considered as major antigens in the diagnosis of allergic anisakiasis caused by A. pegreffii.

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Section: Discussioncontrasting

confidence: 64%

“…It has been observed that Ani s 1 and Ani s 7 have no amino acid sequence similarity with any other allergen able to sensitize humans; and they are also useful for diagnosing infections with Anisakis spp. Furthermore, Gonzalez‐Fernandez et al . recently described a globin in A. simplex ( s. l .…”

Section: Introductionmentioning

confidence: 99%

IgE sensitization to Anisakis pegreffii in Italy: Comparison of two methods for the diagnosis of allergic anisakiasis

Mattiucci

Colantoni

Crisafi

et al. 2017

Parasite Immunology

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“…However, patients with previous gastroallergic anisakiasis reactions do not react to dead larvae or larval proteins administered as a challenge, and a diet excluding raw fish seems to prevent most reactions, with the removal of unfrozen fresh fish preventing additional reactions due to the possibility of the fish not being cooked thoroughly . A wide range of Anisakis allergens have been identified, which include molecules such as protease inhibitors, tropomyosin and haemoglobin …”

Section: Introductionmentioning

confidence: 99%

Anisakis – immunology of a foodborne parasitosis

Nieuwenhuizen

2016

Parasite Immunology

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SummaryAnisakis species are marine nematodes which can cause zoonotic infection in humans if consumed in raw, pickled or undercooked fish and seafood. Infection with Anisakis is associated with abdominal pain, nausea and diarrhoea and can lead to massive infiltration of eosinophils and formation of granulomas in the gastrointestinal tract if the larvae are not removed. Re-infection leads to systemic allergic reactions such as urticarial or anaphylaxis in some individuals, making Anisakis an important source of hidden allergens in seafood. This review summarizes the immunopathology associated with Anisakis infection. Anisakiasis and gastroallergic reactions can be prevented by consuming only fish that has been frozen to −20°C to the core for at least 24 hours before preparation. Sensitization to

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“…l .) [32]. Moreover, the A. pegreffii haemoglobin is an IgG- and IgE-reacting protein in Anisakis -infected mice [33].…”

Section: Discussionmentioning

confidence: 99%

Allergenic activity of Pseudoterranova decipiens (Nematoda: Anisakidae) in BALB/c mice

et al. 2017

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Background Anisakis simplex is the only fishery-product associated parasite causing clinical allergic responses in humans so far. However, other anisakids, due to the presence of shared or own allergens, could also lead to allergic reactions after sensitization. The aim of this study was to determine if Pseudoterranova decipiens belonging to the family Anisakidae has allergenic activity and is able to induce sensitization after oral administration in a murine (BALB/c mice) model.ResultsThe ingestion of A. pegreffii proteins by BALB/c mice, which had been previously sensitized by intraperitoneal inoculation with the corresponding live L3 larvae, triggers signs of allergy within 60 min, whereas P. decipiens did to a lesser extent. Beside symptoms, allergic reactions were furtherly supported by the presence of histamine in sera of sensitized mice. Specific IgG1 and IgE responses were detected in sera of all sensitized mice from week four. Specific IgG2a response was detected in sera from mice sensitized to P. decipiens. After polyclonal or specific activation with anti-CD3/anti-CD28 or antigens, respectively, splenocytes from mice infected i.p. with A. pegreffii or P. decipiens larvae showed significantly higher production of IL-10 than naïve mice. After stimulation with specific antigens, significantly higher IL-5 and IL-13 amounts were produced by specific antigen stimulated splenocytes than by the naïve cells; only P. decipiens proteins induced IFN-ɣ.ConclusionsThe overall results suggest that infection with P. decipiens can sensitize mice to react to subsequent oral challenge with anisakid proteins, as described for A. simplex (sensu stricto) and A. pegreffii infections. The results show that anisakid proteins induce a dominant Th2 response, although P. decipiens could also induce a mixed type 1/type 2 pattern.

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Haemoglobin, a new major allergen of Anisakis simplex

Cited by 33 publications

References 45 publications

IgE sensitization to Anisakis pegreffii in Italy: Comparison of two methods for the diagnosis of allergic anisakiasis

IgE sensitization to Anisakis pegreffii in Italy: Comparison of two methods for the diagnosis of allergic anisakiasis

Anisakis – immunology of a foodborne parasitosis

Allergenic activity of Pseudoterranova decipiens (Nematoda: Anisakidae) in BALB/c mice

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