Haemolymph lipids of healthy and diseased Japanese beetle larvae

Bennett, Glenn A.; Shotwell, Odette L.

doi:10.1016/0022-1910(72)90064-9

Cited by 15 publications

(6 citation statements)

References 29 publications

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“…Rashwan, [30] observed the reduction in total carbohydrate after treatment with rynaxypyr and spinetoram by -73.93 and -54.97% relative to control. Similar, results were observed by [34][35] . A slight reduction of -1.59% in protein content, as its value was reduced from 0.439 in the control to 0.432 mg/ml in treated larvae.…”

Section: Biochemical Changes Changes In Carbohydrates Proteins and Li...supporting

confidence: 91%

Green silver nanoparticles against Helicoverpa armigera and its effects on biochemical, morphological and histological aspects

Soni¹,

Dhiman²

2022

J. Entomol. Zool. Stud.

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The aim of this study was to evaluate the effect of green synthesized silver nanoparticles using aqueous leaf extract of Ricinus communis (castor plant) on the life cycle Helicoverpa armigera. The synthesized AgNPs were identified using different microscopic techniques: UV-Visible spectroscopy, Scanning electron microscopy (SEM), Energy-disruptive X-ray spectroscopy (EDX) and Transmission electron microscopy (TEM). Insecticidal activity of AgNPs was studied using leaf dipping method. The biochemical studies were analyzed at LC50 of AgNPs using different methods (total carbohydrate using phenol-sulphuric acid reaction of Dubois method, total protein using Bradford method, total lipid using sulfo-phospho vanillin of Knight method and non-specific esterases using Van-Asperan method, respectively). UV-vis spectrophotometer confirmed the spectra at 445 nm of AgNPs. Spherical shaped AgNPs of 8.96 nm size were confirmed by TEM and SEM. EDX spectra confirm the chemical composition AgNPs. Higher mortality was observed in 6 th instar larvae (70-80% at LC50 175 ppm) than the other instars. The pupa and adult of H. armigera also exhibited toxicity against the AgNPs. Effect of AgNPs treatment was investigated on the midgut of 5 th instar larva of H. armigera using the transmission electron microscope. Accumulation of AgNPs in cell organelles was confirmed by ultra structural studies of insect midgut cell using TEM. The biochemical components of 5 th instar larvae showed that, the amount of total carbohydrate, total protein, total lipid and non-specific esterases were significantly decreased After 24 h treatment at LC50 of AgNPs. Now, we can summarise that, synthesis of AgNPs using R. communis is a green route to control pest population. The synthesized nanoparticles could be used for the improvement of new botanical nano-insecticide or nano-formulation after successful field trials.

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Section: Biochemical Changes Changes In Carbohydrates Proteins and Li...supporting

confidence: 91%

Green silver nanoparticles against Helicoverpa armigera and its effects on biochemical, morphological and histological aspects

Soni¹,

Dhiman²

2022

J. Entomol. Zool. Stud.

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“…Our results showed a significant increase of lipid at LC 10 and decrease at LC 20 , LC 30 , and LC 40 . Bennett & Shotwell (1972) suggested that the infected larvae might produce enzyme that utilises lipids for energy requirement. Our results also showed a significant decrease of glycogen content in 5 th instar G. pyloalis larvae treated with LC 10 , LC 20 , LC 30 , and LC 40 of spinosad concentrations.…”

Section: Discussionmentioning

confidence: 99%

Sublethal effects of spinosad on some biochemical and biological parameters of Glyphodes pyloalis Walker (Lepidoptera: Pyralidae)

Piri¹,

Sahragard²,

Ghadamyari³

2014

Plant Prot. Sci.

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Piri F., Sahragard A., Ghadamyari M. (2014): Sublethal effects of spinosad on some biochemical and biological parameters of Glyphodes pyloalis Walker (Lepidoptera: Pyralidae). Plant Protect. Sci., 50: 135-144.The susceptibility of G. pyloalis larvae to spinosad was studied using the leaf dip method. Treatment with doses of spinosad sublethal concentrations (LC 10 , LC 20 , LC 30 , LC 40 of 0.026, 0.045, 0.065, 0.090 ppm, respectively) was applied. A significant difference in the effects was observed between the sublethal concentrations (LC 10 , LC 20 , LC 30 , and LC 40 ) and the control in the content of carbohydrate and glycogen, and between the control vs. LC 30 and LC 40 in the content of protein. A significant decrease in glutathione S-transferase activity with the increase of spinosad concentration, no significant differences in the activities of α-and β-esterases, and a significant increase in the enzyme activity of phenoloxidase were observed. Effects of LC 10 and LC 30 spinosad concentrations on some biological parameters showed that percentage of larval pupation and female fecundity significantly decreased in the concentration of LC 30 .

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“…The significant decrease in total lipids may be attributable to the breakdown of lipids into simpler forms that can be utilized for growth as a carbon source. Bennett and Shotwell [38] suggested that for energy requirements, the treated larvae would produce an enzyme that utilizes lipids. El-Aasar et al [39] declared that the reduction in total carbohydrate content of the treated larva might be related to metamorphic changes in the larva.…”

Section: Discussionmentioning

confidence: 99%

The Proficiency of Silver Nanoparticles in Controlling Cotton Leafworm, Spodoptera Littoralis (Boisd.), Under the Laboratory Conditions

Abdou

Zyaan

2023

Egyptian Journal of Zoology

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A new control strategy is required to face the resistance of the cotton leafworm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae), to chemical insecticides, and to decrease the pollution of the environment. Silver nanoparticles (AgNPs) were used to overcome these problems and to control S. littoralis at the immature stage. AgNPs were prepared by the reduction method. The prepared AgNPs were sphere-shaped with an average diameter of 20.73 nm. Several concentrations of AgNPs (50, 100, 200, 400, and 800 ppm) were used as insecticides against the third instar larvae of S. littoralis under laboratory conditions. The feeding treatments and adsorption via the integument produced malformations and morphological changes in the treated S. littoralis larvae. The larvicidal effect of AgNPs recorded LC50 equal to 224.8 ppm after 14 days of the application. Weight loss in the larvae and the pupae after treatment was significant with averages 47.13% and 60.41%, respectively. The treatment produced malformations in the shape and size of larvae and pupae. Some metal-detoxifying enzymes were significantly affected due to the treatment. There were quantitative and qualitative changes in the protein contents, in addition to a significant reduction in the total lipids and total carbohydrates of the treated larvae compared with the untreated larvae. The application of AgNPs against S. littoralis larva, as demonstrated by our findings, paves the way for new pest control options.

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Haemolymph lipids of healthy and diseased Japanese beetle larvae

Cited by 15 publications

References 29 publications

Green silver nanoparticles against Helicoverpa armigera and its effects on biochemical, morphological and histological aspects

Green silver nanoparticles against Helicoverpa armigera and its effects on biochemical, morphological and histological aspects

Sublethal effects of spinosad on some biochemical and biological parameters of Glyphodes pyloalis Walker (Lepidoptera: Pyralidae)

The Proficiency of Silver Nanoparticles in Controlling Cotton Leafworm, Spodoptera Littoralis (Boisd.), Under the Laboratory Conditions

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