Haemonchus contortus Infection Alters Gastrointestinal Microbial Community Composition, Protein Digestion and Amino Acid Allocations in Lambs

Xiang, Hai; Fang, Yi; Tan, Zhiliang; Zhong, Rongzhen

doi:10.3389/fmicb.2021.797746

Cited by 9 publications

(7 citation statements)

References 62 publications

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“…Here, shotgun metagenome was performed to provide information about the structure and function of rumen microbiota. At first, our observations showed that infection significantly changed the structure and decreased the diversity of rumen microbiota, which are in agreement with previous studies that have shown differences in the structure and diversity of ruminal microbiota in lambs infected with GIN (El-Ashram et al 2017 , Corrêa et al 2020 , 2021 , Xiang et al 2022 ). Lower diversity may be associated with acute infection and gut dysbiosis (Cattadori et al 2016 , Peachey et al 2017 ).…”

Section: Discussionsupporting

confidence: 93%

Interaction between methanotrophy and gastrointestinal nematodes infection on the rumen microbiome of lambs

Corrêa,

Fernandes,

Jimenez

et al. 2024

FEMS Microbiology Ecology

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Complex crosstalk occurs between gastrointestinal nematodes and gut symbiotic microbiota, with consequences for animal metabolism. To investigate the connection between methane production and endoparasites, this study evaluated the effect of mixed infection with Haemonchus contortus and Trichostrongylus colubriformis on methanogenic and methanotrophic community in rumen microbiota of lambs using shotgun metagenomic and Real-time quantitative PCR (qPCR). The rumen content was collected from 6 Santa Inês lambs, (7 months old) before and after 42 days infection by esophageal tube. The metagenomic analysis showed that the infection affected the microbial community structure leading to decreased abundance of methanotrophs bacteria, i.e. α-proteobacteria and β-proteobacteria, anaerobic methanotrophic archaea (ANME), protozoa, sulfate-reducing bacteria, syntrophic bacteria with methanogens, geobacter, and genes related to pyruvate, fatty acid, nitrogen, and sulfur metabolisms, ribulose monophosphate cycle and Entner-Doudoroff Pathway. Additionally, the abundance of methanogenic archaea and the mcrA gene did not change. The co-occurrence networks enabled us to identify the interactions between each taxon in microbial communities and to determine the reshaping of rumen microbiome associations by gastrointestinal nematode infection. Besides, the correlation between ANMEs was lower in the animal's post-infection. Our findings suggest that gastrointestinal parasites potentially lead to decreased methanotrophic metabolism-related microorganisms and genes.

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Section: Discussionsupporting

confidence: 93%

Interaction between methanotrophy and gastrointestinal nematodes infection on the rumen microbiome of lambs

Corrêa,

Fernandes,

Jimenez

et al. 2024

FEMS Microbiology Ecology

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“…The PCV and FEC are preferred indicators of gastrointestinal nematode (GIN) infection (Xiang et al, 2021). In this study, the lower PCV and higher FEC observed in infected lambs (I-adlib) compared to those of uninfected counterparts (Un-adlib and Un-restd) indicates clearly that parasite infection was achieved.…”

Section: Effects Of Gin Infection and Breed On Feed Intake Lwg Feed D...mentioning

confidence: 74%

“…However, further reductions in performance due to H. contortus infection are associated to the nutritional cost of infection; i.e., the use of macroand micro-nutrients required for the repair of damaged tissues and the setting of the immune response (Sahoo and Khan, 2016;Meńdez-Ortıź et al, 2019;Ramos-Bruno et al, 2021). A recent study conducted by Xiang et al (2021) concluded that lambs TABLE 6 Mean ( ± least significant difference, LSD) daily methane emission (g/d), methane yield (expressed per kg of intake of dry matter (DMI), organic matter (OMI), digestible DMI (DDMI), digestible organic matter intake (DOMI)) and methane conversion factor (Ym, MJ CH 4 /MJ GEI) by Red Maasai and Dorper lambs infected with H. contortus and fed ad libitum (I-adlib), and uninfected and fed ad libitum (Un-adlib) or restricted (Un-restd) during the balance period (Days 29−33).…”

Section: Effects Of Gin Infection and Breed On Feed Intake Lwg Feed D...mentioning

confidence: 99%

“…Infection by GIN in small ruminants alters substantially the gastrointestinal tract and understanding of the effects of infection on microbiome diversity and function, and ultimately on rate of methanogenesis is still in early development (Li et al, 2016;El-Ashram et al, 2017;Correa et al, 2020;Correâ et al, 2021;Xiang et al, 2021). These studies showed alterations in the ruminal and abomasal microbial functional structure and composition as consequence of GIN infection.…”

Section: Effects Of Gin Infection and Breed On Daily Methane Emission...mentioning

confidence: 99%

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Impact of Haemonchus contortus infection on feed intake, digestion, liveweight gain, and enteric methane emission from Red Maasai and Dorper sheep

Mwangi¹,

Eckard²,

Gluecks³

et al. 2023

Front. Anim. Sci.

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A study was conducted with Red Maasai and Dorper lambs to evaluate the effects of infection with the gastrointestinal nematode (GIN) Haemonchus contortus on feed intake, liveweight gain (LWG), feed energy and nitrogen partitioning, and enteric methane (CH4) emissions. Six- to seven-month-old Red Maasai (n=12) and Dorper (n=12) lambs were randomly allocated to three treatments (n=8, four lambs per breed) in a 2×3 factorial cross-over study over two periods (P1 and P2) of 36 days each. The treatments consisted of three combinations of GIN infection and feeding level: Infected + ad libitum feeding (I-adlib), uninfected + ad libitum feeding (Un-adlib), and uninfected + restricted feeding (Un-restd), across the two breeds. Lambs in the I-adlib group were trickle-infected daily with 1,000 L3 stage larvae of H. contortus for four consecutive days (Days 1–4), whereas lambs in the other experimental treatments were kept GIN free. The feed intake was measured daily. Liveweight (LW), faecal egg counts (FEC), and packed cell volume (PCV) were measured on Day 1 and weekly thereafter. On Days 29–33 total faecal and urine outputs were determined in metabolic crates. The lambs were then housed in respiration chambers for three consecutive days (Days 34–36). There was a washout period of 21 days before P2 started. Uninfected lambs (Un-adlib and Un-restd) had undetectable FEC throughout the study. On Day 36, FEC did not differ between the breeds (P>0.05). Infected lambs (I-adlib) had lower PCV than uninfected (Un-adlib and Un-restd) lambs on day 36. Neither breed nor infection influenced feed and nutrient intake, but as expected, restricted-fed lambs had a lower intake (P<0.05). The LWG of Un-adlib lambs was significantly higher than that of I-adlib and Un-restd lambs (P<0.05), whereas there was no breed difference (P>0.05). Neither breed nor infection affected feed digestibility, nitrogen retention or energy metabolisability (P>0.05). However, feed restriction decreased feed intake, LWG and N retention, whereas feed digestibility and energy metabolisability were unaffected. Neither daily CH4 emissions nor yield (per unit of feed intake) were affected by experimental infection, but Un-restd lambs had lower CH4 emissions per day. Red Maasai lambs had consistently lower daily CH4 emissions and yields than Dorper (P<0.01). This study confirmed the relative resistance of indigenous sheep (Red Maasai) to H. contortus infection, but the increased CH4 emission and yield due to GIN observed in other studies was not confirmed. Further investigations are needed to test whether in environments with multiple stress factors, local or indigenous breeds or their crossbreeds with exotic breeds may be better equipped to sustain production and simultaneously have a reduced carbon footprint than purebred exotic breeds.

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“…Research has demonstrated that Haemonchus contortus has a significant impact on the gastrointestinal microbiota in lambs. It also reduces the α-diversity of the gastrointestinal microbial community in the rumen, abomasum, and duodenum, and disrupts the partitioning of amino acids for protein digestion and absorption ( 16 ). Ascaris lumbricoides , belonging to the superfamily Ascaridoidea along with Anisakis, is also capable of reducing the microbiota diversity of the porcine gut and altering the metabolic potential of the host ( 17 ).…”

Section: Introductionmentioning

confidence: 99%

Modulation of the rat intestinal microbiota in the course of Anisakis pegreffii infection

Zeng,

Li,

et al. 2024

Front. Vet. Sci.

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BackgroundAnisakis are globally distributed, marine parasitic nematodes that can cause human health problems, including symptoms such as vomiting, acute diarrhea, and allergic reactions. As parasitic nematodes that primarily affect the patient’s digestive tract, intestinal helminths can interact directly with the host microbiota through physical contact, chemicals, or nutrient competition. It is widely accepted that the host microbiota plays a crucial role in the regulation of immunity.Materials and methodsNematodes collected from the abdominal cavity of marine fish were identified by molecular biology and live worms were artificially infected in rats. Infection was determined by indirect ELISA based on rat serum and worm extraction. Feces were collected for 16S rDNA-based analysis of microbiota diversity.ResultsMolecular biology identification based on ITS sequences identified the collected nematodes as A. pegreffii. The success of the artificial infection was determined by indirect ELISA based on serum and worm extraction from artificially infected rats. Microbiota diversity analysis showed that a total of 773 ASVs were generated, and PCoA showed that the infected group was differentiated from the control group. The control group contained five characterized genera (Prevotellaceae NK3B31 group, Turicibacter, Clostridium sensu stricto 1, Candidatus Stoquefichus, Lachnospira) and the infected group contained nine characterized genera (Rodentibacter, Christensenella, Dubosiella, Streptococcus, Anaeroplasma, Lactococcus, Papillibacter, Desulfovibrio, Roseburia). Based on the Wilcoxon test, four processes were found to be significant: bacterial secretion system, bacterial invasion of epithelial cells, bacterial chemotaxis, and ABC transporters.ConclusionThis study is the first to analyze the diversity of the intestinal microbiota of rats infected with A. pegreffii and to determine the damage and regulation of metabolism and immunity caused by the infection in the rat gut. The findings provide a basis for further research on host-helminth-microbe correlationships.

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Haemonchus contortus Infection Alters Gastrointestinal Microbial Community Composition, Protein Digestion and Amino Acid Allocations in Lambs

Cited by 9 publications

References 62 publications

Interaction between methanotrophy and gastrointestinal nematodes infection on the rumen microbiome of lambs

Interaction between methanotrophy and gastrointestinal nematodes infection on the rumen microbiome of lambs

Impact of Haemonchus contortus infection on feed intake, digestion, liveweight gain, and enteric methane emission from Red Maasai and Dorper sheep

Modulation of the rat intestinal microbiota in the course of Anisakis pegreffii infection

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