Haemophilus influenzae in children with cystic fibrosis: Antimicrobial susceptibility, molecular epidemiology, distribution of adhesins and biofilm formation

Cardines, Rita; Giufrè, Maria; Pompilio, Arianna; Fiscarelli, Ersilia; Ricciotti, Gabriella; Bonaventura, Giovanni Di; Cerquetti, Marina

doi:10.1016/j.ijmm.2011.08.003

Cited by 50 publications

(57 citation statements)

References 48 publications

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“…A higher prevalence of 17.3% among CF patients over 10 years has been reported by Razvi et al [9]. In 2000 Saiman reported a prevalence of 19% in their study, however in CF patients younger than one year this was 38% [10]. Our overall prevalence is lower, but looking at the recent years of our study (2008 onwards) the percentages are similar to what has been published previously.…”

Section: Discussionsupporting

confidence: 87%

Haemophilus influenzae and Haemophilus parainfluenza in Cystic Fibrosis: 15 Years Experience

Ebbing¹,

Robertson

Robinson

2015

J Medical Microbiol Diagnosis

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Section: Discussionsupporting

confidence: 87%

Haemophilus influenzae and Haemophilus parainfluenza in Cystic Fibrosis: 15 Years Experience

Ebbing¹,

Robertson

Robinson

2015

J Medical Microbiol Diagnosis

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“…Bacterial biofilms have long been linked to persistent infections (Costerton et al, 1999), and biofilm formation has been demonstrated for Haemophilus influenzae (Cardines et al, 2012;Starner et al, 2006), Staphylococcus aureus (Molina et al, 2008) and Pseudomonas aeruginosa (Lutz et al, 2012;Murray et al, 2007) from cystic fibrosis (CF) patients. More recently, the presence of biofilm in the airways of children with non-CF bronchiectasis (culture negative for P. aeruginosa) was demonstrated and was detected more frequently in lavage-2 than in lavage-1 .…”

Section: Introductionmentioning

confidence: 99%

Respiratory bacterial culture from two sequential bronchoalveolar lavages of the same lobe in children with chronic cough

Hare

Marsh

Smith‐Vaughan

et al. 2015

Journal of Medical Microbiology

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Identification of bacteria causing lower-airway infections is important to determine appropriate antimicrobial therapy. Flexible bronchoscopy with bronchoalveolar lavage (BAL) is used to obtain lower-airway specimens in young children. The first lavage (lavage-1) is typically used for bacterial culture. However, no studies in children have compared the detection of cultivable bacteria from sequential lavages of the same lobe. BAL fluid was collected from two sequential lavages of the same lobe in 79 children enrolled in our prospective studies of chronic cough. The respiratory bacteria Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, Staphylococcus aureus and Haemophilus parainfluenzae were isolated and identified using standard published methods. H. influenzae was differentiated from Haemophilus haemolyticus using PCR assays. Lower-airway infection was defined as $10 4 c.f.u. ml 21 BAL fluid. We compared cultivable bacteria from lavage-1 with those from the second lavage (lavage-2) using the k statistic. Lower-airway infections by any pathogen were detected in 46 % of first lavages and 39 % of second lavages. Detection was similar in both lavages for all pathogens; the k statistic was 0.7-0.8 for all bacteria except H. parainfluenzae. Of all infections detected in either lavage, 90 % were detected in lavage-1 and 78 % in lavage-2. However, culture of lavage-2 identified infections that would have been missed in 8 % of children, including infections by additional Streptococcus pneumoniae serotypes. Our findings support the continued use of lavage-1 for bacterial culture; however, culture of lavage-2 may yield additional identifications of bacterial pathogens in lower-airway infections.

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“…13 NTHi colonizes/infects the respiratory tract of more than 20% CF patients early in childhood (with or without Staphylococcus aureus), initiating the inflammatory process distinctive of the disease. 13,14 Colonization with multiple NTHi strains is common, however persistence of the same strain for a period ranging from 1 month to 24 months has been observed in about one third of patients.…”

Section: Non-invasive H Influenzae Infectionsmentioning

confidence: 99%

“…11,12 NTHi colonization/infection is also quite common in young children with cystic fibrosis, contributing to the disease morbidity. 13,14 On the basis of the above reasoning, NTHi has been increasingly recognized as emerging pathogen. 9,15,16 An effective vaccine against NTHi is not currently available.…”

mentioning

confidence: 99%

Why we need a vaccine for non-typeable Haemophilus influenzae

Cerquetti

Giufrè

2016

Human Vaccines & Immunotherapeutics

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Haemophilus influenzae in children with cystic fibrosis: Antimicrobial susceptibility, molecular epidemiology, distribution of adhesins and biofilm formation

Cited by 50 publications

References 48 publications

Haemophilus influenzae and Haemophilus parainfluenza in Cystic Fibrosis: 15 Years Experience

Haemophilus influenzae and Haemophilus parainfluenza in Cystic Fibrosis: 15 Years Experience

Respiratory bacterial culture from two sequential bronchoalveolar lavages of the same lobe in children with chronic cough

Why we need a vaccine for non-typeable Haemophilus influenzae

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