Haemophilus influenzae strains possess variations in the global transcriptional profile in response to oxygen levels and this influences sensitivity to environmental stresses

Jiang, Donald; Tikhomirova, Alexandra; Kidd, Stephen P.

doi:10.1016/j.resmic.2015.08.004

Cited by 2 publications

(3 citation statements)

References 40 publications

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“…In standard laboratory conditions, H. influenzae is cultured in a carbon dioxide enriched (5%) aerobic environment, a procedure also used in the present study. When comparing our data to previous studies on the metabolic flexibility of H. influenzae when exposed to different oxygen levels (18,29,(33)(34)(35) (see Supplement File 5: H. influenzae metabolism when exposed to different oxygen levels) our results indicated contradictory conclusions about the oxygen availability for microbes in the lung. Two significant genes for the action of competence, dprA and rec2, were among the genes that had the highest relative expression in vivo.…”

Section: Polland Et Al 2023contrasting

confidence: 55%

TheHaemophilus influenzaein vivo gene expression reveals major clues about bacterial central metabolism, acquisition of trace elements, and other essential pathways during infection of the human lung

Polland

Paulsson

2023

Preprint

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Haemophilus influenzaeis a major cause of community and hospital acquired pneumonia. While extensively studied in various laboratory models, less is known about how this species persists and causes infection inside the human lung. We present the first study on the H. influenzae in vivo transcriptome during pneumonia, and contrast this with isolates cultured in vitro under standard laboratory conditions. Patients with pneumonia were recruited from emergency departments and intensive care units in a Swedish referral hospital during 2018-2020 (n=102). Duplicates of lower respiratory samples were collected for bacterial culture and RNA-extraction. Patient samples withH. influenzae(n=18) from which bacterial mRNA of adequate quantity and quality could be extracted (n=8) underwent RNA-sequencing, along with duplicates of lab-cultured counterparts (n=7). The transcripts were aligned to core and pan genomes created from 15 reference strains. While in vitro bacteria clustered tightly in principal component analyses of core genome (n=1067) expression, the in vivo samples displayed diverse transcriptomic signatures and did not group with their lab-grown counterparts. In total, 328 core genes were significantly differentially expressed between in vitro and in vivo conditions. The most upregulated genes in vivo included the transferrin-acquisition genes tbp1 and fbpA and the reductase gene msrAB involved in stress response pathways. Biosynthesis of nucleotides/purines, response-to-heat systems, and molybdopterin-scavenging processes were also significantly upregulated in vivo. Major metabolic pathways and iron-sequestering processes were downregulated in vivo. In conclusion, extensive transcriptomic differences were found between bacteria collected in the human lung during pneumonia and isogenic bacteria cultured in vitro.

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Section: Polland Et Al 2023contrasting

confidence: 55%

TheHaemophilus influenzaein vivo gene expression reveals major clues about bacterial central metabolism, acquisition of trace elements, and other essential pathways during infection of the human lung

Polland

Paulsson

2023

Preprint

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“…Moreover, H. influenzae transits between niches within the host that differ in oxygen levels, and the ArcAB two-component regulatory system controls gene expression in response to different respiratory conditions of growth. Gene expression profiling contributed not only to identify the H. influenzae ArcA regulon upon anaerobic growth [75] , but also to decipher changes in H. influenzae transcriptional responses upon changes in oxygen availability, showing that, in high oxygen, arginine uptake, arginine/aspartate metabolism, and glutamate pathways leading into the Krebs cycle are commonly up-regulated [76] .…”

Section: Unraveling Features Of the H Influenzae -Host Interplay By Genome-wide Gene Expression Profilingmentioning

confidence: 99%

“…Tn-seq based in vitro genetic screenings [69] , [70] , [71] , [72] , [73] C. Genome-wide gene expression profiling C1. Bacterial transcriptomic responses to changing environmental conditions [75] , [76] , [77] , [78] , [79] , [82] , [84] , [87] C2. Bacterial transcriptomic responses to gene inactivation [35] , [53] , [54] , [55] , [59] , [80] , [81] , [83] C3.…”

Section: Introductionmentioning

confidence: 99%

Learning from –omics strategies applied to uncover Haemophilus influenzae host-pathogen interactions: Current status and perspectives

López-López

Gil-Campillo

Díez-Martínez³

et al. 2021

Computational and Structural Biotechnology Journal

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Haemophilus influenzae has contributed to key bacterial genome sequencing hallmarks, as being not only the first bacterium to be genome-sequenced, but also starring the first genome-wide analysis of chromosomes directly transformed with DNA from a divergent genotype, and pioneering Tn-seq methodologies. Over the years, the phenomenal and constantly evolving development of –omic technologies applied to a whole range of biological questions of clinical relevance in the H. influenzae -host interplay, has greatly moved forward our understanding of this human-adapted pathogen, responsible for multiple acute and chronic infections of the respiratory tract. In this way, essential genes, virulence factors, pathoadaptive traits, and multi-layer gene expression regulatory networks with both genomic and epigenomic complexity levels are being elucidated. Likewise, the unstoppable increasing whole genome sequencing information underpinning H. influenzae great genomic plasticity, mainly when referring to non-capsulated strains, poses major challenges to understand the genomic basis of clinically relevant phenotypes and even more, to clearly highlight potential targets of clinical interest for diagnostic, therapeutic or vaccine development. We review here how genomic, transcriptomic, proteomic and metabolomic-based approaches are great contributors to our current understanding of the interactions between H. influenzae and the human airways, and point possible strategies to maximize their usefulness in the context of biomedical research and clinical needs on this human-adapted bacterial pathogen.

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Haemophilus influenzae strains possess variations in the global transcriptional profile in response to oxygen levels and this influences sensitivity to environmental stresses

Cited by 2 publications

References 40 publications

TheHaemophilus influenzaein vivo gene expression reveals major clues about bacterial central metabolism, acquisition of trace elements, and other essential pathways during infection of the human lung

TheHaemophilus influenzaein vivo gene expression reveals major clues about bacterial central metabolism, acquisition of trace elements, and other essential pathways during infection of the human lung

Learning from –omics strategies applied to uncover Haemophilus influenzae host-pathogen interactions: Current status and perspectives

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