Haemophilus is overrepresented in the nasopharynx of infants hospitalized with RSV infection and associated with increased viral load and enhanced mucosal CXCL8 responses

Ederveen, Thomas H. A.; Ferwerda, Gerben; Ahout, Inge M. L.; Vissers, Marloes; Groot, Ronald de; Boekhorst, Jos; Timmerman, Harro M.; Huynen, Martijn A.; Hijum, Sacha A. F. T. van; Jonge, Marien I. de

doi:10.1186/s40168-017-0395-y

Cited by 67 publications

(80 citation statements)

References 52 publications

(66 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Here, the authors show a shift from a Staphylococcus dominated microbiome in uninfected infants to a Moraxella, Streptococcus, Corynebacterium, Haemophilus, and Dolosigranulum dominated respiratory microbiome in RV and RSV infected patients. The predominance of Haemophilus in RSV infected lung has been corroborated by an independent study by Ederveen et al (109). There are interesting points to be taken from these studies.…”

Section: Viral Infections Of the Lungmentioning

confidence: 60%

Lung inflammation and disease: A perspective on microbial homeostasis and metabolism

et al. 2018

View full text Add to dashboard Cite

Summary It is now well appreciated that the human microbiome plays a significant role in a number of processes in the body, significantly affecting its metabolic, inflammatory and immune homeostasis. Recent research has revealed that almost every mucosal surface in the human body is associated with a resident commensal microbiome of its own. While the gut microbiome and its role in regulation of host metabolism along with its alteration in a disease state has been well studied, there is a lacuna in understanding the resident microbiota of other mucosal surfaces. Among these, the scientific information on the role of lung microbiota in pulmonary diseases is currently severely limited. Historically, lungs have been considered to be sterile and lung diseases have only been studied in the context of bacterial pathogenesis. Recently however, studies have revealed a resilient microbiome in the upper and lower respiratory tracts and there is increased evidence on its central role in respiratory diseases. Knowledge of lung microbiome and its metabolic fallout (local and systemic) is still in its nascent stages and attracting immense interest in recent times. In this review, we will provide a perspective on lung-associated metabolic disorders defined for lung diseases (e.g. COPD, Asthma, respiratory depression due to infection) and correlate it with lung microbial perturbation. Such perturbations may be due to altered biochemical or metabolic stress as well. Finally, we will draw evidence from microbiome and classical microbiology literature to demonstrate how specific lung morbidities associate with specific metabolic characteristics of the disease, and with the role of microbiome in this context.

show abstract

Section: Viral Infections Of the Lungmentioning

confidence: 60%

Lung inflammation and disease: A perspective on microbial homeostasis and metabolism

et al. 2018

View full text Add to dashboard Cite

show abstract

“…24,26,27 In this study, we observed that in infants who later developed recurrent wheezing, there was a higher abundance of Proteobacteria. 30 Although the exact mechanism(s) by which the airway microbiota influences the pattern of airway inflammatory response during RSV bronchiolitis is unclear, it may be partly due to the higher level of LPS produced by these Gram-negative pathogenic bacteria observed in this study. Airway chronic bacterial colonization and endotoxin exposure might induce persistent activation of the innate immune system resulting in the production of pro-inflammatory cytokines in airway.…”

Section: Discussionmentioning

confidence: 82%

Airway microbiome, host immune response and recurrent wheezing in infants with severe respiratory syncytial virus bronchiolitis

Zhang

et al. 2020

Pediatric Allergy Immunology

View full text Add to dashboard Cite

Background: Early interactions between respiratory viruses and microbiota mightmodulate host immune responses and subsequently contribute to later development of recurrent wheezing and asthma in childhood. We aimed to study the possible association between respiratory microbiome, host immune response, and the development of recurrent wheezing in infants with severe respiratory syncytial virus (RSV) bronchiolitis.Methods: Seventy-four infants who were hospitalized at Beijing Children's Hospital during an initial episode of severe RSV bronchiolitis at 6 months of age or less were included and followed up until the age of 3 years. Sputum samples were collected, and their microbiota profiles, LPS, and cytokines were analyzed by 16S rRNA-based sequencing, ELISA, and multiplex immunoassay, respectively.Results: Twenty-six (35.1%) infants developed recurrent wheezing by the age of 3 years, and 48 (64.9%) did not. The relative abundance of Haemophilus, Moraxella, and Klebsiella was higher in infants who later developed recurrent wheezing than in those who did not (LDA score >3.5). Airway levels of LPS (P = .003), CXCL8 (P = .004), CCL5 (P = .029), IL-6 (P = .004), and IL-13 (P < .001) were significantly higher in infants who later developed recurrent wheezing than in those who did not. Moreover, high airway abundance of Haemophilus was associated with CXCL8 (r = 0.246, P = .037) level, and that of Moraxella was associated with IL-6 level (r = 0.236, P = .046) and IL-10 level (r = 0.266, P = .024). Conclusion:Our study suggests that higher abundance of Haemophilus and Moraxella in airway microbiome might modulate airway inflammation during severe RSV bronchiolitis in infancy, potentially contributing to the development of subsequent recurrent wheezing in later childhood. K E Y W O R D S airway inflammation, children, lipopolysaccharide, recurrent wheezing, respiratory microbiome, respiratory syncytial virus S U PP O RTI N G I N FO R M ATI O N Additional supporting information may be found online in the Supporting Information section. How to cite this article: Zhang X, Zhang X, Zhang N, et al. Airway microbiome, host immune response and recurrent wheezing in infants with severe respiratory syncytial virus bronchiolitis. Pediatr Allergy Immunol. 2020;31:281-289.

show abstract

“…During viral respiratory infections, alpha-diversity and richness were suggested to be lower [55] and to potentially drive symptom severity [47], in particular when RSV is the aetiological agent [58]. However, these findings are not consistently found [16,59,60].…”

Section: Respiratory Microbiota Impacts Bronchiolitis In Childrenmentioning

confidence: 98%

Relationship between nasopharyngeal microbiota and patient’s susceptibility to viral infection

Dubourg

Edouard

Raoult

2019

Expert Review of Anti-infective Therapy

View full text Add to dashboard Cite

Introduction: The burden of respiratory viral infections is a global public health concern with significant mortality, morbidity, and economic impact. While Koch's postulate led to considering only the etiological agent, numerous works have demonstrated that commensal microbes could contribute to both the susceptibility and the severity of these infections, in particular those of the nasopharynx. Areas covered: Herein, we first propose to briefly recall the historical background that led to considering microbes inhabiting the nasopharyngeal microbiota as a potential contributor to human viral infections. We describe the evolution of the normal nasopharyngeal microbiota composition over time, especially during the first year of life. We aimed to resume the changes of the nasopharyngeal microbiota during viral respiratory infections. We also develop how nasopharyngeal microbiota could contribute to the acquisition of respiratory viral infections. We finally provide the potential therapeutic perspectives deriving from these findings. Expert opinion: Prospective studies focusing on children have identified that nasopharyngeal microbiota composition is associated with predisposition to acute respiratory illness and bronchiolitis, while data are scarce regarding adults. For the latter, further works are needed, in particular as a part of the multi-OMICS approach that should probably be performed in conjunction with gut microbiota studies.

show abstract

Haemophilus is overrepresented in the nasopharynx of infants hospitalized with RSV infection and associated with increased viral load and enhanced mucosal CXCL8 responses

Cited by 67 publications

References 52 publications

Lung inflammation and disease: A perspective on microbial homeostasis and metabolism

Lung inflammation and disease: A perspective on microbial homeostasis and metabolism

Airway microbiome, host immune response and recurrent wheezing in infants with severe respiratory syncytial virus bronchiolitis

Relationship between nasopharyngeal microbiota and patient’s susceptibility to viral infection

Contact Info

Product

Resources

About