Hahella chejuensis gen. nov., sp. nov., an extracellular-polysaccharide-producing marine bacterium.

Lee, Hong Kum; Chun, Jongsik; Moon, Eun Young; Ko, Sung Hwan; Lee, Deuk Soo; Lee, Hyun Sang; Bae, Kyung Sook

doi:10.1099/00207713-51-2-661

Cited by 100 publications

(63 citation statements)

References 30 publications

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“…increased at an interface between the oxidized surface and the anaerobic layer of black mud, assimilating the reduced product from the lower anoxic zone (6). Another unique oxycline phylotype was distantly related (88% similarity) to Hahella chejuensis, a facultative anaerobe capable of using several different carbohydrates as a carbon source (38). The two other unique oxycline phylotypes were related to Cytophaga spp.…”

Section: Discussionmentioning

confidence: 89%

Depth Distribution of Microbial Diversity in Mono Lake, a Meromictic Soda Lake in California

Humayoun

Bano

Hollibaugh

2003

Appl Environ Microbiol

329

268

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We analyzed the variation with depth in the composition of members of the domain Bacteria in samples from alkaline, hypersaline, and currently meromictic Mono Lake in California. DNA samples were collected from the mixolimnion (2 m), the base of the oxycline (17.5 m), the upper chemocline (23 m), and the monimolimnion (35 m). Composition was assessed by sequencing randomly selected cloned fragments of 16S rRNA genes retrieved from the DNA samples. Most of the 212 sequences retrieved from the samples fell into five major lineages of the domain Bacteria: ␣-and ␥-Proteobacteria (6 and 10%, respectively), Cytophaga-FlexibacterBacteroides (19%), high-G؉C-content gram-positive organisms (Actinobacteria; 25%), and low-G؉C-content gram-positive organisms (Bacillus and Clostridium; 19%). Twelve percent were identified as chloroplasts. The remaining 9% represented ␤-and ␦-Proteobacteria, Verrucomicrobiales, and candidate divisions. Mixolimnion and oxycline samples had low microbial diversity, with only 9 and 12 distinct phylotypes, respectively, whereas chemocline and monimolimnion samples were more diverse, containing 27 and 25 phylotypes, respectively. The compositions of microbial assemblages from the mixolimnion and oxycline were not significantly different from each other (P ‫؍‬ 0.314 and 0.877), but they were significantly different from those of chemocline and monimolimnion assemblages (P < 0.001), and the compositions of chemocline and monimolimnion assemblages were not significantly different from each other (P ‫؍‬ 0.006 and 0.124). The populations of sequences retrieved from the mixolimnion and oxycline samples were dominated by sequences related to high-G؉C-content grampositive bacteria (49 and 63%, respectively) distributed in only three distinct phylotypes, while the population of sequences retrieved from the monimolimnion sample was dominated (52%) by sequences related to low-G؉C-content gram-positive bacteria distributed in 12 distinct phylotypes. Twelve and 28% of the sequences retrieved from the chemocline sample were also found in the mixolimnion and monimolimnion samples, respectively. None of the sequences retrieved from the monimolimnion sample were found in the mixolimnion or oxycline samples. Elevated diversity in anoxic bottom water samples relative to oxic surface water samples suggests a greater opportunity for niche differentiation in bottom versus surface waters of this lake.Understanding the factors that control the composition and diversity of microbial communities is important to understanding how bacterial populations function to facilitate biogeochemical processes. Mono Lake is an ideal site for such studies because it has strong geochemical gradients, a simple food web, and a relatively simple microbial community. Mono Lake is an alkaline (pH 9.8), hypersaline (84 to 94 g liter Ϫ1 ) soda lake located east of the Sierra Nevada mountains, approximately 160 km south of Lake Tahoe, Calif. Because of the contrast between the density of saline lake water and freshwater flowing into soda lakes, ...

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Section: Discussionmentioning

confidence: 89%

Depth Distribution of Microbial Diversity in Mono Lake, a Meromictic Soda Lake in California

Humayoun

Bano

Hollibaugh

2003

Appl Environ Microbiol

329

268

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“…During optimization of EPS, the selection of carbon source plays an important role. When growth medium was amended with sucrose, the highest EPS yield was obtained from Hahella chejuensis isolated from Cheju Island, Republic of Korea [27]. The haloalkaliphilic microorganism, Halomonas alkaliantartica strain CRSS which was isolated from a saline lake in Antarctica produced maximum EPS when acetate was used as a carbon source [28].…”

Section: Introductionmentioning

confidence: 99%

Production and Characterization of Exopolysaccharide From Marine Moderately Halophilic Bacterium Halomonas Smyrnensis SVD Iii

Deshmukh

Kanekar²,

Bhadekar

2017

Int J Pharm Pharm Sci

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Objective: To study 1) Optimization of nutritional and environmental parameters to enhance the yield of EPS by Halomonas smyrnensis SVD III isolated from seawater, West Coast of Maharashtra, India and 2) Purification and characterization of the EPS produced. Methods:The isolate was grown in Sehgal and Gibbons (SG) medium broth supplemented with 3% glucose, at 37 °C, 120 rpm for 7 d. Optimization of different parameters was carried out with one factor at a time approach. EPS was isolated from cell-free supernatant of the culture broth by centrifugation and precipitation using chilled ethanol, after removal of proteins by trichloroacetic acid (TCA) treatment. Characterization of the purified EPS was carried out with respect to fourier-transform infrared (FTIR) spectrum, 1 H nuclear magnetic resonance (NMR) spectrum and mass spectrometry (MS) analysis.Results: Two-fold increase in the yield of EPS (23 g/l) by the selected isolate was obtained by using culture conditions as 10% inoculum size having cell density of 10 7 cells/ml, pH 6, incubation temperature 45 °C, 3% carbohydrate, 0.5% yeast extract as nitrogen source, 20% salt concentration and 7 d of incubation period. Characterization of the purified EPS suggested the presence of dominated glycosidic linkages and heptasaccharide nature of the molecule. As the present strain is halophilic, 20% NaCl was found to be optimum. Conclusion:Optimization studies resulted in two-fold increase in the yield of EPS which is of heptasaccharide nature.

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“…Growth occurs aerobically, although some species are facultative anaerobes, for example Endozoicomonas euniceicola, Endozoicomonas gorgoniicola and Kistimonas scapharcae. The type genus, Hahella, first proposed by Lee et al (2001) …”

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confidence: 99%

Allohahella marinimesophila gen. nov., sp. nov., isolated from seawater and reclassification of Hahella antarctica as Allohahella antarctica comb. nov.

Han

Zhao

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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Allohahella marinimesophila gen. nov., sp. nov., isolated from seawater and reclassification of Hahella antarctica as Allohahella antarctica comb. nov. A Gram-stain-negative, non-endospore-forming, strictly aerobic, irregular rod-shaped bacterium without flagellum, designated strain H94 T , was isolated by the high-throughput cultivation method from seawater of an amphioxus breeding zone in the coastal region of Qingdao, China.Growth was observed at 4-37 C (optimum 28 C), at pH 6.0-10.0 (optimum pH 7.0) and in the presence of 1-12 % (w/v) NaCl (optimum 1-2 %). The predominant cellular fatty acids were C 18 : 1 !9c, C 16 : 0 and C 16 : 1 !9c. The polar lipids comprised phosphatidylethanolamine, phosphatidylglycerol and an unidentified phosphoglycolipid. The major respiratory quinone was ubiquinone-9 (Q-9). The genomic DNA G+C content of strain H94 T was 56.2 mol%. The family Hahellaceae belongs to the order Oceanospirillales, within the class Gammaproteobacteria, and was circumscribed in 2005 on the basis of phylogenetic analysis of 16S rRNA gene sequences (Garrity et al., 2005). Five genera have been assigned to the family Hahellaceae: the type genus Hahella (Lee et al., 2001), Zooshikella (Yi et al., 2003), et al., 2006), Endozoicomonas (Kurahashi & Yokota, 2007) and Kistimonas (Choi et al., 2010). At the time of writing, the family Hahellaceae contains 14 species. All known species of this family are Gram-stain-negative, rod-shaped, with an absolute NaCl requirement for growth, and there are even some extremely halophilic species, such as Halospina denitrificans. Growth occurs aerobically, although some species are facultative anaerobes, for example Endozoicomonas euniceicola, Endozoicomonas gorgoniicola and Kistimonas scapharcae. The type genus, Hahella, first proposed by Lee et al. (2001)

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Hahella chejuensis gen. nov., sp. nov., an extracellular-polysaccharide-producing marine bacterium.

Cited by 100 publications

References 30 publications

Depth Distribution of Microbial Diversity in Mono Lake, a Meromictic Soda Lake in California

Depth Distribution of Microbial Diversity in Mono Lake, a Meromictic Soda Lake in California

Production and Characterization of Exopolysaccharide From Marine Moderately Halophilic Bacterium Halomonas Smyrnensis SVD Iii

Allohahella marinimesophila gen. nov., sp. nov., isolated from seawater and reclassification of Hahella antarctica as Allohahella antarctica comb. nov.

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