Hair analysis for veterinary drug monitoring in livestock production

Gratacós-Cubarsí, Marta; Castellari, Massimo; Valero, A.; Garcı́a-Regueiro, J.A.

doi:10.1016/j.jchromb.2006.03.007

Cited by 69 publications

(46 citation statements)

References 56 publications

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“…Human hair and animal fur have served as matrices for biomonitoring of chemicals such as nicotine, other drugs and narcotics, and hormones (14–16). However, those exposures are at levels exceeding the levels of HAAs by at least three orders of magnitude.…”

Section: Introductionmentioning

confidence: 99%

Biomonitoring of Carcinogenic Heterocyclic Aromatic Amines in Hair: A Validation Study

Bessette

Yasa

Dunbar

et al. 2009

Chem. Res. Toxicol.

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A facile method was established to measure heterocyclic aromatic amines (HAAs) accumulated in human hair and rodent fur. The samples were digested by base hydrolysis, and the liberated HAAs were isolated by tandem solvent/solid-phase extraction. Quantification was done by liquid chromatography/tandem mass spectrometry, using a triple stage quadrupole mass spectrometer in the selected reaction monitoring mode. In a pilot study of 12 human volunteers, 2-amino-1-methyl-6-phenylimidazo [4,5-b]pyridine (PhIP) was detected in hair of six meat-eaters at levels ranging from 290 to 890 pg/g hair. 2-Amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx) and 2-amino-9H-pyrido [2,3-b]indole (AαC) were below the limit of quantification (LOQ) (50 pg/g hair) in hair from meat-eaters and six vegetarians. PhIP was detected in the hair from one vegetarian, and at level just above the LOQ (65 pg/g hair), indicating PhIP exposure occurs primarily through meat consumption. The levels of PhIP in hair samples from two meat-eaters varied by less than 24% over a 6-month interval, signifying that the exposure to PhIP and its accumulation in hair are relatively constant over time. In a controlled feeding study, female C57BL/6 mice were given these HAAs in their drinking water for 1 month, at six daily dose concentrations ranging from 0, 0.080 to 800 µg/kg body weight. PhIP was detected in fur of mice at all doses, whereas AαC and MeIQx were detected in fur at dosages ≥0.8 µg AαC/kg body weight and ≥8 µg MeIQx/kg body weight. There was a strong positive relationship between dosage and each of the HAAs accumulated in fur and their DNA adducts formed in liver and colon (p-values <0.0001); however, the levels of HAA in fur did not correlate to the levels of DNA adducts after adjustment of dose. Thus, hair appears to be a promising long-lived biomarker with by which we can assess the exposure to PhIP, a potential human carcinogen. Figure S-1); the LC-ESI/MS/MS chromatograms of HAAs accumulated in rodent fur following subchronic treatment with HAAs at a dose of 80 µg HAA/day/kg body wt ( Figure S-2); the MS 3 product ion spectrum of guanyl-C8-AαC and [ 13 C 6 ]-guanyl-C8-AαC in liver of mice treated subchronically with 800 µg AαC/day/kg body wt ( Figure S-3); the MS 3 product ion spectrum of guanyl-C8-PhIP and [ 13 C 6 ]-guanyl-C8-PhIP in liver of mice treated subchronically with 800 µg PhIP/day/kg body wt ( Figure S-4); the MS 3 product ion spectrum of guanyl-C8-MeIQx and guanyl-C8-[ 2 H 3 C]-MeIQx in liver of mice treated subchronically with 800 µg MeIQx/ day/kg body wt ( Figure S

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Section: Introductionmentioning

confidence: 99%

Biomonitoring of Carcinogenic Heterocyclic Aromatic Amines in Hair: A Validation Study

Bessette

Yasa

Dunbar

et al. 2009

Chem. Res. Toxicol.

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“…Fortunately, hair may be a suitable sample for drug monitoring in livestock production because its collection is quick and simple, and the drug concentration in hair is stable [9]. Research on agonists found in cattle and human hair has shown that hair can be considered a good matrix to determine the presence of ␤-agonist in living animals [10][11][12][13][14][15].…”

Section: Ractopamine (Rac) 4-[3-[[2-hydroxy-2-(4-hydroxyphenyl) Ethymentioning

confidence: 99%

Determination of ractopamine in animal hair: Application to residue depletion in sheep and residue monitoring

Suo

Zhao

Wang

et al. 2014

Journal of Chromatography B

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“…As hormonal esters do not naturally occur in the animal organism, the detection of these synthetic substances in the body of an animal provides irrefutable evidence of the abuse of these promoters. Although the administration of esters of natural hormones can be detected through hair analysis (Duffy et al, 2008;Grataco s-Cubarsi et al, 2006;Pedreira et al, 2007;Rambaud et al, 2005), it has been very difficult to detect intact steroid esters in body fluids or tissues. It is likely that esters quickly hydrolyze in the body of the animal, releasing the corresponding natural hormone (Stolker et al, 2009).…”

Section: Synthetic and Semi-synthetic Steroidsmentioning

confidence: 99%

Natural Hormones in Food-Producing Animals:Legal Measurements and Analytical Implications

Regal¹,

Cepeda²,

Fente³

2012

Food Production - Approaches, Challenges and Tasks

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Hair analysis for veterinary drug monitoring in livestock production

Cited by 69 publications

References 56 publications

Biomonitoring of Carcinogenic Heterocyclic Aromatic Amines in Hair: A Validation Study

Biomonitoring of Carcinogenic Heterocyclic Aromatic Amines in Hair: A Validation Study

Determination of ractopamine in animal hair: Application to residue depletion in sheep and residue monitoring

Natural Hormones in Food-Producing Animals:Legal Measurements and Analytical Implications

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