Hair follicle dermal sheath cells: unsung participants in wound healing

Jahoda, Colin A.B.; Reynolds, Amanda J.

doi:10.1016/s0140-6736(01)06532-1

Cited by 221 publications

(172 citation statements)

References 11 publications

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“…Given the multipotent nature of SKPs, they might also contribute to the mesodermal components of the dermis, including dermal fibroblasts, either during normal physiological turnover or during wound healing. In this regard, cells of the follicle dermal papillae and sheath have previously been proposed to participate in wound healing in the skin (Jahoda & Reynolds 2001).…”

Section: Implications and Unresolved Questions (A)mentioning

confidence: 99%

Multipotent skin-derived precursors: adult neural crest-related precursors with therapeutic potential

Fernandes

Toma

Miller

2007

Phil. Trans. R. Soc. B

117

102

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We previously made the surprising finding that cultures of multipotent precursors can be grown from the dermis of neonatal and adult mammalian skin. These skin-derived precursors (SKPs) display multi-lineage differentiation potential, producing both neural and mesodermal progeny in vitro, and are an apparently novel precursor cell type that is distinct from other known precursors within the skin. In this review, we begin by placing these findings within the context of the rapidly evolving stem cell field. We then describe our recent efforts focused on understanding the developmental biology of SKPs, discussing the idea that SKPs are neural crest-related precursors that (i) migrate into the skin during embryogenesis, (ii) persist within a specific dermal niche, and (iii) play a key role in the normal physiology, and potentially pathology, of the skin. We conclude by highlighting some of the therapeutic implications and unresolved questions raised by these studies.

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Section: Implications and Unresolved Questions (A)mentioning

confidence: 99%

Multipotent skin-derived precursors: adult neural crest-related precursors with therapeutic potential

Fernandes

Toma

Miller

2007

Phil. Trans. R. Soc. B

117

102

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“…The epithelialmesenchymal interactions with the underlying dermal papilla play a pivotal role in embryonic hair genesis [24], the regulation of the postnatal hair follicle cyclical activity, and the repair of wounded skin [25][26][27][28]. Dermal papilla cells (DPCs) have shown inductive properties in the generation of bioengineered skin substitutes from basal epidermal stem cells [29].…”

Section: Introductionmentioning

confidence: 99%

Dermal Papilla Cells Improve the Wound Healing Process and Generate Hair Bud-Like Structures in Grafted Skin Substitutes Using Hair Follicle Stem Cells

Leirós

Kusinsky

Drago³

et al. 2014

Stem Cells Translational Medicine

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Tissue-engineered skin represents a useful strategy for the treatment of deep skin injuries and might contribute to the understanding of skin regeneration. The use of dermal papilla cells (DPCs) as a dermal component in a permanent composite skin with human hair follicle stem cells (HFSCs) was evaluated by studying the tissue-engineered skin architecture, stem cell persistence, hair regeneration, and graft-take in nude mice. A porcine acellular dermal matrix was seeded with HFSCs alone and with HFSCs plus human DPCs or dermal fibroblasts (DFs). In vitro, the presence of DPCs induced a more regular and multilayered stratified epidermis with more basal p63-positive cells and invaginations. The DPC-containing constructs more accurately mimicked the skin architecture by properly stratifying the differentiating HFSCs and developing a well-ordered epithelia that contributed to more closely recapitulate an artificial human skin. This acellular dermal matrix previously repopulated in vitro with HFSCs and DFs or DPCs as the dermal component was grafted in nude mice. The presence of DPCs in the composite substitute not only favored early neovascularization, good assimilation and remodeling after grafting but also contributed to the neovascular network maturation, which might reduce the inflammation process, resulting in a better healing process, with less scarring and wound contraction. Interestingly, only DPC-containing constructs showed embryonic hair bud-like structures with cells of human origin, presence of precursor epithelial cells, and expression of a hair differentiation marker. Although preliminary, these findings have demonstrated the importance of the presence of DPCs for proper skin repair.

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“…Although others have shown that UDS cells can regenerate papillae (7), differences between lower and upper DS cell populations have never been fully investigated. We recently hypothesized that dermal cells of the follicle, in particular the DS cells, have another stem cell function in skin in the context of wound healing (8). This is based on an emerging body of indirect evidence that DS cells participate in wound repair and because they resemble wound healing fibroblasts, specifically in that they express alpha smooth muscle actin (9).…”

Section: Introductionmentioning

confidence: 99%

Plasticity of hair follicle dermal cells in wound healing and induction

Gharzi

Reynolds

Jahoda

2003

Experimental Dermatology

230

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Gharzi A, Reynolds AJ, Jahoda CAB. Plasticity of hair follicle dermal cells in wound healing and induction. Exp Dermatol 2003: 12: 126-136. # Blackwell Munksgaard, 2003 Abstract: The capacity of adult hair follicle dermal cells to participate in new follicle induction and regeneration, and to elicit responses from diverse epithelial partners, demonstrates a level of developmental promiscuity and influence far exceeding that of interfollicular fibroblasts. We have recently suggested that adult follicle dermal cells have extensive stem or progenitor cell activities, including an important role in skin dermal wound healing. Given that up to now tissue engineered skin equivalents have several deficiencies, including the absence of hair follicles, we investigated the capacity of follicle dermal cells to be incorporated into skin wounds; to form hair follicles in wound environments; and to create a hair follicle-derived skin equivalent. In our study, we implanted rat follicle dermal cells labelled with a vital dye into ear and body skin wounds. We found that they were incorporated into the new dermis in a manner similar to skin fibroblasts, but that lower follicle dermal sheath also assimilated into hair follicles. Using different combinations of follicle dermal cells and outer root sheath epithelial cells in punch biopsy wounds, we showed that new hair follicles were formed only with the inclusion of intact dermal papillae. Finally by combining follicle dermal sheath and outer root sheath cells in organotypic chambers, we created a skin equivalent with characteristic dermal and epidermal architecture and a normal basement membrane -the first skin to be produced entirely from hair follicle cells. These data support the hypothesis that follicle dermal cells may be important in wound healing and demonstrate their potential usefulness in human skin equivalents and skin substitutes. While we have made progress towards producing skin equivalents that contain follicles, we suggest that the failure of cultured dermal papilla cells to induce follicle formation in wounds illustrates the complex role the follicle dermis may play in skin. We believe that it demonstrates a genuine dichotomy of activity for follicle cells within skin.

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Hair follicle dermal sheath cells: unsung participants in wound healing

Cited by 221 publications

References 11 publications

Multipotent skin-derived precursors: adult neural crest-related precursors with therapeutic potential

Multipotent skin-derived precursors: adult neural crest-related precursors with therapeutic potential

Dermal Papilla Cells Improve the Wound Healing Process and Generate Hair Bud-Like Structures in Grafted Skin Substitutes Using Hair Follicle Stem Cells

Plasticity of hair follicle dermal cells in wound healing and induction

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