Hair Growth Effect of Emulsion Extracted Brevilin A, a JAK3 Inhibitor, from Centipeda minima

Abstract

<jats:p>Janus kinase 3 (JAK3) inhibitors have been used effectively in the treatment of several cases of alopecia universalis and its variants. Our study aims to evaluate whether the emulsion extract of brevilin A from Centipeda minima (CMX) stimulates hair regrowth in a clinical trial, as a JAK3 inhibitor, combined with network pharmacology-based analysis. CMX showed potent inhibition of JAK3 in a concentration-dependent manner. Significant differences in total hair count, terminal hair count, and anagen hair count from the baseline to 24 weeks were observed between the placebo and CMX subjects. The gene set enrichment analysis showed that the targets of CMX are mainly associated with the JAK-STAT signaling pathway, cytokine–cytokine receptor interactions, and the MAPK signaling pathway. This study suggests that the medicinal herbal extract CMX is useful in the treatment of mild to moderate vertex balding that contribute to the visible improvements in hair growth observed in treated patients.</jats:p>

Hair Growth Stimulation Effect of Centipeda minima Extract: Identification of Active Compounds and Anagen-Activating Signaling Pathways

“…The amounts of major compounds of the CMX were determined by HPLC-UV (Thermo Scientific Dionex Ultimate 3000, Thermo Fisher Scientific, Sunnyvale, CA, USA). For the HPLC conditions, refer to the previous study [11]. Filtered samples (10 µL) were injected into a SUPERSIL column ODS-I (250 mm × 4.6 mm, 5.0 µm), and the column temperature was maintained at 40 • C. For the mobile phase, 0.1% formic acid in distilled water was used as Solvent A and methanol was used as Solvent B.…”

“…The representative HPLC chromatograms of CMX are shown in Figure 1, and the four major compounds of CMX are marked with their retention time. These four compounds have been identified by HPLC-Q-TOF-MS as arnicolide D, arnicolide C, microhelenin C, and brevilin A, and their chemical structures are shown in Figure 2 [11]. The concentrations of these four major compounds were calculated by the calibration curve of the standard (1-100 µg/mL); the contents of each component in CMX are summarized in Table 2.…”

Alopecia areata treated with topical and systemic brevilin A: A case series

“…CM is used in Chinese medicine for several diseases, such as headache, allergies, cough, asthma, and malaria, from thousands of years 9 …”

“…To date, there has been only one clinical trial to evaluate the stimulatory effect of emulsion extract of brevilin A Centipeda minima (CM) on hair regrowth 9 . In this study, 72 patients with vertex balding were randomly assigned to apply either emulsion extracted brevilin A or placebo.…”

“…To date, there has been only one clinical trial to evaluate the stimulatory effect of emulsion extract of brevilin A Centipeda minima (CM) on hair regrowth. 9 In this study, 72 patients with vertex balding were randomly assigned to apply either emulsion extracted brevilin A or placebo. After 24 weeks of therapy, the group of patients treated with CM experienced a higher total hair count, terminal hair count ad anagen hair count compared to placebo group.…”