Hair Growth-Promoting Activity of Hair Tonic containing Delipidated Ethanol Extract of Capsicum frutescens L. Leaves on Male Rabbit (Oryctolagus cuniculus)

Abstract

Background: One of the common features of unhealthy hair is abnormal hair loss which can lead to alopecia (baldness). Capsicum frutescens L.leaves contain saponins to increase blood flow to hair follicles and flavonoids that inhibit free radicals that cause hair loss.Hairtonic is a cosmetic product that deals with hair problems because it is easy to apply and not sticky. Objective: to formulate Hair tonic from delipidated ethanol extract of Capsicum frutescens L. leaves and to observe its hair growth-promoting effectivenesson male rabbits (Oryctolagus cuniculus). Methods: Capsicum frutescens L. leaves were extracted with ethanolby using maceration method,delipidation extract was obtained by using liquid-liquid separation technique with n-hexane as nonpolar phase, hair tonic formulations with 5%, 10% and 20% were prepared by using dissolving and stirring without heating. Results: Hair Tonic were light to dark brown, pH range was 4.5 - 5.9; Averagehair growth for 3 weeks of normal control was 6.88±0.50; negative control was 7.59±0.94; Positive control was 34.54±0.93; Hair Tonic with delipidated ethanol extract at 5%, 10% and 20%, respectively, were 18.28±0.74; 24.53±0.88; and 33.33±0.86. Conclusion: Hair Tonic with delipidated ethanol extract of Capsicum frutescens L. at 5%, 10% and 20% has potential for hair-promoting growth.Hair Tonic with delipidated ethanol extract of Capsicum frutescens L. at 5%, 10% and 20% has potential for hair-promoting growth when compared to placebo (Negative control) and Normal control. With concentration of 20%, the formulation of Hair Tonic Delipidated Ethanol Extract of Capsicum frutescens L. Leaves has hair growth-promoting activity roughly equivalent to hair tonic containing 2% Minoxidil (Positive control).

Can Plant Extracts Help Prevent Hair Loss or Promote Hair Growth? A Review Comparing Their Therapeutic Efficacies, Phytochemical Components, and Modulatory Targets

“…Mice and rats have often been used as animal models to evaluate hair growth whereas rabbits or guinea pigs have rarely been used [107,111,112,115,116]. Many studies have used C57BL/6 mice, which have the advantage of being easy to observe with the naked eye due to their dark fur color.…”