Hair shaft miniaturization causes stem cell depletion through mechanosensory signals mediated by a Piezo1-calcium-TNF-α axis

Xie, Yuhua; Chen, Daoming; Jiang, Kaiju; Song, Ling; Qian, Nannan; Du, Yingxue; Yong, Yang; Wang, Fengchao; Chen, Ting

doi:10.1016/j.stem.2021.09.009

Cited by 47 publications

(34 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… Next depending on the purpose of the experiment, removing unwanted hair follicles might be needed. For our study ( Xie et al., 2022 ), we intended to study catagen-telogen hair follicles. In this case, the long anagen hair follicles will obscure the view of neighboring catagen-telogen hair follicles that are much shorter.…”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

“…Anagen hair follicles are much longer than catagen-telogen hair follicles; and anagen hair follicles contain enlarged matrix structure at the bottom, which is absent in catagen-telogen hair follicles ( Müller-Röver et al., 2001 ). For the purpose of our study ( Xie et al., 2022 ), we intended to study catagen-telogen hair follicles (outlined in white dotted line). In this case, the long anagen hair follicles (outlined in yellow dotted line) will obscure the view of neighboring catagen-telogen hair follicles that are much shorter.…”

Section: Expected Outcomesmentioning

confidence: 99%

“…The applications of the protocol include visualization of calcium signaling in other cell types with Cre/CreER lines or analyzing other cellular features with different reporter lines. For complete details on the use and execution of this protocol, please refer to Xie et al. (2022) .…”

mentioning

confidence: 99%

See 2 more Smart Citations

Mouse tail skin wholemount staining and intravital calcium imaging

Xie

Chen

2022

STAR Protocols

Self Cite

View full text Add to dashboard Cite

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

Section: Expected Outcomesmentioning

confidence: 99%

See 1 more Smart Citation

Mouse tail skin wholemount staining and intravital calcium imaging

Xie

Chen

2022

STAR Protocols

Self Cite

View full text Add to dashboard Cite

“…An alternative model for the loss of aged HFSCs was recently proposed ( Figure 1D ): mechanical compression induces Piezo channel activation, calcium influx, and subsequently HFSC apoptosis ( Xie et al, 2022 ). While the loss of inhibitory K6+ niche cells is known to cause HFSC activation ( Hsu et al, 2011 ), in this context, mechanical cues play a dominant function, since the reinsertion of the hair shaft alone without the K6+ cells returned bulge HFSCs to quiescence ( Xie et al, 2022 ). Corroborating this model, repetitive hair depilation induces hair follicle aging ( Keyes et al, 2013 ; Lay et al, 2016 ), likely evoking mechanical compressions and the Piezo1-calcium-TNFα axis ( Xie et al, 2022 ).…”

Section: Molecular Mechanisms Underlying Hair Follicle Miniaturizationmentioning

confidence: 99%

Toward Elucidating Epigenetic and Metabolic Regulation of Stem Cell Lineage Plasticity in Skin Aging

Lyu

2022

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Skin is the largest organ in human body, harboring a plethora of cell types and serving as the organismal barrier. Skin aging such as wrinkling and hair graying is graphically pronounced, and the molecular mechanisms behind these phenotypic manifestations are beginning to unfold. As in many other organs and tissues, epigenetic and metabolic deregulations have emerged as key aging drivers. Particularly in the context of the skin epithelium, the epigenome and metabolome coordinately shape lineage plasticity and orchestrate stem cell function during aging. Our review discusses recent studies that proposed molecular mechanisms that drive the degeneration of hair follicles, a major appendage of the skin. By focusing on skin while comparing it to model organisms and adult stem cells of other tissues, we summarize literature on genotoxic stress, nutritional sensing, metabolic rewiring, mitochondrial activity, and epigenetic regulations of stem cell plasticity. Finally, we speculate about the rejuvenation potential of rate-limiting upstream signals during aging and the dominant role of the tissue microenvironment in dictating aged epithelial stem cell function.

show abstract

“…One report compared HF stem cell functions between 2 month and 24-month-old mice and found that the old mice HF showed a longer telogen phase, defective proliferation, and shorter hair growth phase [109]. Loss of HF stem cells is also associated with age-related hair shaft miniaturization [125]. Another typically aged phenotype related to hair is hair thinning, graying, or hair loss.…”

Section: Chronological Aging and Epidermal Stem Cellmentioning

confidence: 99%

Keratinocyte Stem Cells: Role in Aging

Ansary¹,

Kamiya²,

Ohtsuki³

2022

Keratinocyte Biology - Structure and Function in the Epidermis

View full text Add to dashboard Cite

Stem cells located in the skin are responsible for continual regeneration, wound healing, and differentiation of different cell lineages of the skin. The three main locations of skin stem cells are the epidermis, dermis, and hair follicles. The keratinocyte stem cells are located in the epidermal basal layer (the interfollicular stem cells), hair follicle bulge region (the hair follicle stem cells), and sebaceous glands (the sebaceous gland stem cells) and are responsible for the epidermal proliferation, differentiation, and apoptosis. The interfollicular (IF) stem cells are responsible for epidermis regeneration by proliferating basal cells that attach to the underlying basement membrane and with time they exit from the cell cycle, start terminal differentiation, and move upward to form the spinous, the granular, and the stratum corneum layers. The hair follicle (HF) stem cells are responsible for hair regeneration and these stem cells undergo a cycle consists three stages; growth cycles (anagen), degeneration (catagen), and relative resting phase (telogen). The sebaceous gland (SG) stem cells located in between the hair follicle bulge and the gland and are responsible for producing the entire sebaceous gland which secretes oils to moisture our skin. The role of epidermal stem cells is extremely crucial because they produce enormous numbers of keratinocytes over a lifetime to maintain epidermal homeostasis. However, the age-associated changes in the skin; for example; alopecia, reduced hair density, gray or thin hair, reduced wound healing capacity are related to skin stem cells’ decline functionality with age.

show abstract

Hair shaft miniaturization causes stem cell depletion through mechanosensory signals mediated by a Piezo1-calcium-TNF-α axis

Cited by 47 publications

References 62 publications

Mouse tail skin wholemount staining and intravital calcium imaging

Mouse tail skin wholemount staining and intravital calcium imaging

Toward Elucidating Epigenetic and Metabolic Regulation of Stem Cell Lineage Plasticity in Skin Aging

Keratinocyte Stem Cells: Role in Aging

Contact Info

Product

Resources

About