Hairpin DNA-Functionalized Gold Colloids for the Imaging of mRNA in Live Cells

Jayagopal, Ashwath; Halfpenny, Kristin C.; Perez, Jonas W.; Wright, David W.

doi:10.1021/ja102585v

Cited by 165 publications

(124 citation statements)

References 43 publications

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“…52 It is interesting that AuNP-DNA conjugates can be efficiently taken up by cells. Jayagopal et al 53 presented a strategy for live cell imaging of messenger RNA using DNA hairpin functionalized AuNPs, which shows high promise for real-time analysis of mRNA transport and processing in live cells.…”

Section: Scaffolded Biosensors H Pei Et Almentioning

confidence: 99%

Scaffolded biosensors with designed DNA nanostructures

et al. 2013

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In addition to its fundamental function as a genetic code carrier, the utilization of DNA in various material applications has been actively explored over the past several decades. DNA is intrinsically an excellent type of self-assembly nanomaterial owing to its predictable base-pairing, high chemical stability and the convenience it possesses for synthesis and modification. Because of these unparalleled properties, DNA is widely used as excellent recognition elements in biosensors and as unique building blocks in nanodevices. A critical challenge in surface-based DNA biosensors lies in the reduced accessibility of target molecules to the DNA probes arranged on heterogeneous surfaces, especially when compared to probe-target recognition in homogeneous solutions. To improve the recognition abilities of these heterogeneous surface-confined DNA probes, much effort has been devoted to controlling the surface chemistry, conformation and packing density of the probe molecules, as well as the size and geometry of the surface. In this review, we aim to summarize the recent progress on the improvement of the probetarget recognition properties by introducing DNA nanostructure scaffolds. A range of new strategies have proven to provide a significantly enhanced range in the spatial positioning and the accessibility of the probes to the surface over previously reported linear structures. We will also describe the applications of DNA nanostructure scaffold-based biosensors. INTRODUCTIONDetection of nucleic acids (DNA or RNA) is an integral step in many applications, including in clinical diagnosis, environmental monitoring and antibioterrorism. 1 With these applications in mind, significant efforts have been made to develop sensitive, selective, rapid and cost-effective DNA (or RNA) biosensors. In parallel, DNAbased devices have also attracted a significant, recent interest owing to their promising applications in nanoelectronics, 2,3 biomolecular computations, 4-8 cellular imaging 9 and drug delivery. [10][11][12] In a typical design of DNA biosensors and nanodevices, oligonucleotides are often used as the molecular recognition layer. Because DNA is comprised of only four bases with A-T and G-C pairing, DNA hybridization processes are highly predictable and can be finely tuned with a rational design of the sequence. In addition, DNA oligonucleotides are usually easy to design, chemically stable and cost-effective.Understanding the physical structure of a DNA probe immobilized on a surface is critical in applications using DNA as a molecular recognition element. The properties of the DNA molecular recognition layer (such as selectivity, sensitivity and reproducibility) highly depend on the structure of the self-assembled DNA film. Modeling studies with thiolated DNA have demonstrated that efficient hybridization occurs in the well-controlled condition of surface-attached probes with large interprobe distances and upright conformations. [13][14][15][16][17][18][19][20][21][22][23][24]

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Section: Scaffolded Biosensors H Pei Et Almentioning

confidence: 99%

Scaffolded biosensors with designed DNA nanostructures

et al. 2013

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“…According to the cytotoxicity assay (Figure 4), A549 cell growth was negligibly influenced by the treatment of cells with noncomplementary S-MBMFs, indicating the superior biocompatibility of our system compared to some metal nanoparticle systems. [17] However, their treatment with S-MBMFs resulted in marked inhibition of cell proliferation in a dose-dependent manner, suggesting that MBMFs can be applied as an antisense therapy for cancer cells with high expression level of c-raf-1 mRNA.…”

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confidence: 99%

DNA Micelle Flares for Intracellular mRNA Imaging and Gene Therapy

Chen

Jiménez

et al. 2013

Angewandte Chemie

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Multifunctional DNA micelles: Molecular beacon micelle flares (MBMFs), based on diacyllipidmolecular beacon conjugate (L-MB) self-assembly, have been developed for combined mRNA detection and gene therapy. The advantages of these micelle flares include easy probe synthesis, efficient cellular uptake, enhanced enzymatic stability, high signal-to-background ratio, excellent target selectivity, and superior biocompatibility. In addition, these probes possess a hydrophobic cavity that can be used for additional hydrophobic agents, holding great promise for constructing an all-in-one nucleic acid probe.

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“…72 Similarly, GNP-Poly (para-phenyleneethynylene) could efficiently identify both Gram-positive and negative bacteria based on the differential response by each bacteria. 73 In another study, GNPs functionalized with hairpin DNA was used to image live HEp-2 cells infected with Respiratory syncytial virus 74 Another immunoassay based on multi-functionalized GNPs was developed by using antibodies against protein A, a cell wall protein of the bacterium Staphylococcus aureus, to detect it in food samples. 75 A gold nanoparticle based chemiluminescence assay was designed for the detection of Staphylococcus enterotoxin B (SEB).…”

Section: Detection Of Microorganismsmentioning

confidence: 99%