Hairpin-shaped DNA Templated Copper Na nopar tides for Fluorescence Detection of Adenosine Triphosphate Based on Ligation-mediated Exonuclease Cleavage

Zhu, Wenqing; Dai, Liyan; Liu, Zengchen; Yang, Weijie; Zhao, Chuan‐Zhen; Li, Yanxia; Chen, Yahong

doi:10.2116/analsci.33.203

Cited by 7 publications

(2 citation statements)

References 44 publications

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“…Compared with other metal nanoparticles (i.e., silver nanoparticles and gold nanoparticles), copper nanoparticles (CuNPs) are extensively used in various bioanalysis due to their distinctive properties, such as faster synthesis, less toxicity, low-cost and lager MegaStokes shift [23][24][25]. As of now, CuNPs are used for developing various reporter systems for label-free sensing [26][27][28][29][30][31]. Wang et al reported an efficient technique for microRNA detection using CuNPs based on rolling circle replication [32].…”

Section: Introductionmentioning

confidence: 99%

A Fluorescent Biosensor for Streptavidin Detection Based on Double-Hairpin DNA-Templated Copper Nanoparticles

et al. 2023

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In this paper, we developed a sensitive, label-free and facile fluorescent strategy for detecting streptavidin (SA) based on double-hairpin DNA-templated copper nanoparticles (CuNPs) and terminal protection of small molecule-linked DNA. Herein, a special DNA hairpin probe was designed and synthesized, which contained two poly T single-stranded loops and a nick point in the middle of the stem. Inspired by the concept of the terminal protection interaction, the specific binding of SA to the biotinylated DNA probe can prevent the exonuclease degradation and keep the integrity of DNA probe, which can be used for synthesizing fluorescent CuNPs as a template. Conversely, the DNA probe would be digested by exonucleases and therefore, would fail to form CuNPs without SA. After systematic optimization, the detection range of SA concentration is from 0.5 to 150 nM with a low detection limit of 0.09 nM. Additionally, the proposed method was also successfully applied in the biological samples. Finally, the proposed method is sensitive, effective and simple, and can be potentially applied for predicting diseases and discovering new drugs.

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Section: Introductionmentioning

confidence: 99%

A Fluorescent Biosensor for Streptavidin Detection Based on Double-Hairpin DNA-Templated Copper Nanoparticles

et al. 2023

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“…Zhu et al reported a fluorescent sensing method for adenosine triphosphate (ATP) based on ligation-mediated exonuclease cleavage. 12 The detection mechanism of this method is based on a nick sealing by T4 DNA ligase in the presence of cofactor ATP. Owing to the ATP, a DNA probe is not cleaved by exonuclease III (Exo III), and fluorescent copper nanoparticles (CuNPs) are formed.…”

mentioning

confidence: 99%

DNA-based Fluorescent Sensors

Soh

2018

ANAL. SCI.

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515Fluorescent sensors are useful tools that can detect the target analyte with high sensitivity. To date, various fluorescent sensors have been developed for applications in biological 1 and environmental fields 2 etc. In order to design fluorescent sensors, specific recognition for the target analyte is important. DNA, which is a significant molecule in biology, can be a functional sensing element because the molecule has sophisticated molecular-recognition ability.3 Single-stranded DNA (ssDNA) recognizes the complementary sequence strictly. Some kind of DNA selectively captures the target molecule as an aptamer. In addition, DNA is susceptible to modifications and cleavages by DNA-related enzymes in a rigorous manner. By utilizing these appealing features, many DNA-based fluorescent sensors have been reported in recent years.Yang et al. reported on a fluorescent sensor for kanamycin using the DNA aptamer and G-quadruplex DNA. 4 The sensor consists of two ssDNA, P1 and P2. When the kanamycin is added to the P1-P2 complex, P2, a DNA aptamer of kanamycin, binds to kanamycin, and P1 is released from the complex. The released P1 then forms G-quadruplex DNA that binds to N-methyl mesoporphyrin IX (NMM), resulting in a fluorescence enhancement. A fluorescent sensor for hemin based on competitive binding to G-quadruplex DNA between hemin and protoporphyrin IX (PPIX) was also reported.5 Hg 2+ fluorescent sensors, in which G-quadruplex structures are induced by the formation of T-Hg-T mismatches (T, thymine base), were reported using NMM 6 and thioflavin T (ThT) 7 as signal molecules, respectively. A fluorescent sensor for target DNA was reported based on the binding of ThT with G-quadruplex DNA. 8The stem-loop structure of DNA is also useful for fluorescent sensing. Sawamura et al. developed a fluorescence quenching assay for the detection of point mutations based on a ligase detection reaction (LDR). In positive control (LDR using mutant DNA), two primers are ligated to produce a hairpin-like molecular beacon, and the fluorescence is quenched. A fluorescent sensor for the detection of Hg 2+ was reported, in which a stem-loop structure is cancelled by the formation of a T-Hg-T coordination structure in the presence of ssDNA. 10A fluorescent sensor for target DNA and deoxyribonuclease I was developed based on cancellation of the hairpin structure of DNA on quantum dots (QDs). 11The combined use of the G-quadruplex and hairpin structure is also useful to construct fluorescent sensors. [6][7][8] Nucleases are applied to the construction of a DNA-based fluorescent sensing system. Zhu et al. were also developed. In addition, other DNA-based fluorescent sensors were also reported. Tsuchiya et al. developed fluorescent aptamer sensors that can detect interferon-γ (IFN-γ) and adenine compounds on cells.17 A detection method for cancer mutation based on LDR was reported using fluorescence resonance energy transfer from a donor QD to an acceptor fluorescent dye.18 A fluorescent sensor for Ag + was developed by utilizing specifi...

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Ellman’s method-based determination of acibenzolar-S-methyl in tobacco by transesterification with methanol and SABP2-catalyzed hydrolysis

Yang

Sun

et al. 2022

ANAL. SCI.

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Hairpin-shaped DNA Templated Copper Na nopar tides for Fluorescence Detection of Adenosine Triphosphate Based on Ligation-mediated Exonuclease Cleavage

Cited by 7 publications

References 44 publications

A Fluorescent Biosensor for Streptavidin Detection Based on Double-Hairpin DNA-Templated Copper Nanoparticles

A Fluorescent Biosensor for Streptavidin Detection Based on Double-Hairpin DNA-Templated Copper Nanoparticles

DNA-based Fluorescent Sensors

Ellman’s method-based determination of acibenzolar-S-methyl in tobacco by transesterification with methanol and SABP2-catalyzed hydrolysis

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