Hairy Garlic (Allium subhirsutum) from Sicily (Italy): LC-DAD-MSn Analysis of Secondary Metabolites and In Vitro Biological Properties

Sut, Stefania; Maggi, Filippo; Bruno, Sara; Badalamenti, Natale; Quassinti, Luana; Bramucci, Massimo; Beghelli, Daniela; Lupidi, Giulio; Dall’Acqua, Stefano

doi:10.3390/molecules25122837

Cited by 24 publications

(11 citation statements)

References 57 publications

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“…As shown in Figure 1 , the most abundant compound is 14, followed by 10, then 1, 3 and 4. Results are comparable to a previous study using LC-DAD-MS [ 31 ].…”

Section: Resultssupporting

confidence: 87%

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Allium subhirsutum L. as a Potential Source of Antioxidant and Anticancer Bioactive Molecules: HR-LCMS Phytochemical Profiling, In Vitro and In Vivo Pharmacological Study

et al. 2020

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This study investigated Allium subhirsutum L. (AS) anticancer and antioxidant effects and inhibition of tumor angiogenesis in a murine model of skeletal metastases due to inoculation of Walker 256/B cells. Phytochemical composition of AS extract (ASE) was studied by High Resolution-Liquid Chromatography Mass Spectroscopy (HR-LCMS). Total phenolic and flavonoid contents (TPC and TFC) were determined. In vitro, the antioxidant properties were evaluated by reducing power and antiradical activity against DPPH. Cancer cells’ proliferation, apoptosis, metastatic development and angiogenesis were evaluated using Walker 256/B and MatLyLu cells. The p-coumaric acid was the major phenolic acid (1700 µg/g extract). ASE showed high levels of TPC and TFC and proved potent antioxidant effects. ASE inhibited Walker 256/B and MatLyLu cells’ proliferation (Half-maximal inhibitory concentration: IC50 ≃ 150 µg/mL) and induced apoptosis. In silico and in vivo assays confirmed these findings. ASE effectively acts as a chemo-preventive compound, induces apoptosis and attenuates angiogenesis and osteolytic metastases due to Walker 256/B malignant cells.

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“…As shown in Figure 1 , the most abundant compound is 14, followed by 10, then 1, 3 and 4. Results are comparable to a previous study using LC-DAD-MS [ 31 ].…”

Section: Resultssupporting

confidence: 87%

“…Moreover, ASE demonstrated a potential antiradical scavenging activity ( Table 4 ). This potential antioxidant effect was previously attributed to garlic species [ 35 ] and was able to protect cellular senescence in UVB-exposed skin cells [ 36 ] and potent biological activities related to the AS phytochemical composition [ 31 ].…”

Section: Resultsmentioning

confidence: 93%

Allium subhirsutum L. as a Potential Source of Antioxidant and Anticancer Bioactive Molecules: HR-LCMS Phytochemical Profiling, In Vitro and In Vivo Pharmacological Study

et al. 2020

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“…Allium subhirsutum L. is an aromatic plant known since antiquity. It was investigated in terms of its potential antioxidant components such as flavonoids and polyphenols [ 10 , 11 , 21 ]. Previous studies reported that it is a potential source of anticancer and antioxidant molecules [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

The Role of Allium subhirsutum L. in the Attenuation of Dermal Wounds by Modulating Oxidative Stress and Inflammation in Wistar Albino Rats

et al. 2021

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In our study, Allium subhirsutum L. (AS) was investigated to assess its phenolic profile and bioactive molecules including flavonoids and organosulfur compounds. The antioxidant potential of AS and wound healing activity were addressed using skin wound healing and oxidative stress and inflammation marker estimation in rat models. Phytochemical and antiradical activities of AS extract (ASE) and oil (ASO) were studied. The rats were randomly assigned to four groups: group I served as a control and was treated with simple ointment base, group II was treated with ASE ointment, group III was treated with ASO ointment and group IV (reference group; Ref) was treated with a reference drug “Cytolcentella® cream”. Phytochemical screening showed that total phenols (215 ± 3.5 mg GAE/g) and flavonoids (172.4 ± 3.1 mg QE/g) were higher in the ASO than the ASE group. The results of the antioxidant properties showed that ASO exhibited the highest DPPH free radical scavenging potential (IC50 = 0.136 ± 0.07 mg/mL), FRAP test (IC50 = 0.013 ± 0.006 mg/mL), ABTS test (IC50 = 0.52 ± 0.03 mg/mL) and total antioxidant capacity (IC50 = 0.34 ± 0.06 mg/mL). In the wound healing study, topical application of ASO performed the fastest wound-repairing process estimated by a chromatic study, percentage wound closure, fibrinogen level and oxidative damage status, as compared to ASE, the Cytolcentella reference drug and the untreated rats. The use of AS extract and oil were also associated with the attenuation of oxidative stress damage in the wound-healing treated rats. Overall, the results provided that AS, particularly ASO, has a potential medicinal value to act as effective skin wound healing agent.

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“…•− ), and hydroxyl radical (HO • ), are commonly produced by various biochemical reactions, and can produce, if present in high concentrations, serious damage to DNA, proteins, lipids, thus causing aging and cell death [131]. Scientific research has been addressed towards the study of new antioxidant compounds, obtained both from the natural source [132][133][134][135][136] and chemical-synthetic procedures [137], able to prevent and control the treatment of oxidative stress diseases, including cancer, cardiovascular and neurodegenerative diseases. It is also known that the antioxidant activity of a plant extract is highly correlated with the presence of phenolic compounds [138], and especially of phenolic acids [139].…”

Section: Antioxidant Activitymentioning

confidence: 99%

Ethnobotany, Phytochemistry, Biological, and Nutritional Properties of Genus Crepis—A Review

Badalamenti

Sottile

Bruno

2022

Plants

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The genus Crepis L., included within the Asteraceae family, has a very wide distribution, expanding throughout the northern hemisphere, including Europe, northern Africa, and temperate Asia. This genus has a fundamental value from biodynamic and ecological perspectives, with the different species often being chosen for soil conservation, for environmental sustainability, and for their attraction towards pollinating species. Furthermore, various species of Crepis have been used in the popular medicine of several countries as medicinal herbs and food since ancient times. In most cases, the species is consumed either in the form of a decoction, or as a salad, and is used for its cardiovascular properties, as a digestive, for problems related to sight, for the treatment of diabetes, and for joint diseases. This literature review, the first one of the Crepis genus, includes publications with the word ‘Crepis’, and considers the single metabolites identified, characterised, and tested to evaluate their biological potential. The various isolated compounds, including in most cases sesquiterpenes and flavonoids, were obtained by extracting the roots and aerial parts of the different species. The secondary metabolites, extracted using traditional (solvent extraction, column chromatography, preparative thin layer chromatography, preparative HPLC, vacuum liquid chromatography), and modern systems such as ultrasounds, microwaves, etc., and characterised by mono- and bi- dimensional NMR experiments and by HPLC-MS, have a varied application spectrum at a biological level, with antimicrobial, antioxidant, antidiabetic, antitumor, antiviral, antiulcer, phytotoxic, and nutritional properties having been reported. Unfortunately, in vitro tests have not always been accompanied by in vivo tests, and this is the major critical aspect that emerges from the study of the scientific aspects related to this genus. Therefore, extensive investigations are necessary to evaluate the real capacity of the different species used in food, and above all to discover what the different plants that have never been analysed could offer at a scientific level.

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Hairy Garlic (Allium subhirsutum) from Sicily (Italy): LC-DAD-MSn Analysis of Secondary Metabolites and In Vitro Biological Properties

Cited by 24 publications

References 57 publications

Allium subhirsutum L. as a Potential Source of Antioxidant and Anticancer Bioactive Molecules: HR-LCMS Phytochemical Profiling, In Vitro and In Vivo Pharmacological Study

Allium subhirsutum L. as a Potential Source of Antioxidant and Anticancer Bioactive Molecules: HR-LCMS Phytochemical Profiling, In Vitro and In Vivo Pharmacological Study

The Role of Allium subhirsutum L. in the Attenuation of Dermal Wounds by Modulating Oxidative Stress and Inflammation in Wistar Albino Rats

Ethnobotany, Phytochemistry, Biological, and Nutritional Properties of Genus Crepis—A Review

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