Hairy root culture technology: applications, constraints and prospect

Gantait, Saikat; Mukherjee, Eashan

doi:10.1007/s00253-020-11017-9

Cited by 51 publications

(18 citation statements)

References 198 publications

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“…Escalated production of emblicanin-A and emblicanin-B can be attained via cell suspension culture, added with elicitors of biotic and abiotic origin. Genetic transformation like hairy root culture via Agrobacterium rhizogenes serves as a promising tool for enhancing the metabolite profile of any medicinal plant (Gantait and Mukherjee 2021a) and this strategy is yet to be explored in E. officinalis. Other novel genetic transformation approaches like electroporation (direct) method or indirect via vector mediated using A. tumifaciens or other related vectors are left unexplored to date.…”

Section: Discussionmentioning

confidence: 99%

Advances in biotechnology of Emblica officinalis Gaertn. syn. Phyllanthus emblica L.: a nutraceuticals-rich fruit tree with multifaceted ethnomedicinal uses

et al. 2021

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Emblica officinalis Gaertn. syn. Phyllanthus emblica L., universally known as ‘Amla’ or ‘Aonla’ or ‘Indian gooseberry’, is a popular fruit tree belonging to the family Euphorbiaceae and order Geraniales. It is said to be the very first tree that originated on earth, as claimed by age-old Indian mythology. Almost all parts of the tree i.e., root, bark, leaf, flower, fruit and seed are utilized in Ayurvedic and Unani medicinal formulations to improve the overall digestive process, decrease fever, act as a blood purifier, relieve asthma and cough, improve heart health, etc. This tree contains major secondary metabolites like emblicanin-A and emblicanin-B, and also is an affluent source of vitamin-C. Additionally, some other secondary metabolites like tannins, gallic acid, pyrogallol, and pectin are also present in significant amounts. Conventional propagation has been improved via suitable interventions of agrotechnology both in production and protection areas. However, the rate of propagation remains slower; therefore, attempts have been made for biotechnological advancements on E. officinalis. The present review makes an attempt to highlight the botanical description, geographical distribution, ethnopharmacological importance, conventional propagation and protection of this medicinal tree, describing the in vitro-based plant organ and tissue culture methods like direct and indirect organogenesis and somatic embryogenesis along with interventions of molecular marker-based biotechnology and nanotechnology. Further, the prospect of the yet-to-be-explored biotechnological methods for secondary metabolite enhancement like cell suspension, protoplast culture, genetic transformation, etc. and their potential for enhanced emblicanin production have also been discussed in this appraisal.

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Section: Discussionmentioning

confidence: 99%

Advances in biotechnology of Emblica officinalis Gaertn. syn. Phyllanthus emblica L.: a nutraceuticals-rich fruit tree with multifaceted ethnomedicinal uses

et al. 2021

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“…The ability of Rhizobium rhizogenes (also known as Agrobacterium rhizogenes ) [ 7 ] to transfer and permanently incorporate a foreign genetic material into the plant genomic DNA has also been used to study the regulatory mechanisms of plant metabolism [ 6 , 8 , 9 ]. Additionally, the enzymatic activity of HRs may be utilized for biotransformation and phytoremediation processes [ 10 , 11 , 12 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Hairy Root Cultures as a Source of Polyphenolic Antioxidants: Flavonoids, Stilbenoids and Hydrolyzable Tannins

Malarz

Michalska

Yudina

et al. 2022

Plants

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Due to their chemical properties and biological activity, antioxidants of plant origin have gained interest as valuable components of the human diet, potential food preservatives and additives, ingredients of cosmetics and factors implicated in tolerance mechanisms against environmental stress. Plant polyphenols are the most prominent and extensively studied, albeit not only group of, secondary plant (specialized) metabolites manifesting antioxidative activity. Because of their potential economic importance, the productive and renewable sources of the compounds are desirable. Over thirty years of research on hairy root cultures, as both producers of secondary plant metabolites and experimental systems to investigate plant biosynthetic pathways, brought about several spectacular achievements. The present review focuses on the Rhizobium rhizogenes-transformed roots that either may be efficient sources of plant-derived antioxidants or were used to elucidate some regulatory mechanisms responsible for the enhanced accumulation of antioxidants in plant tissues.

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“…Many published review articles and a wide range of scientific reports notify that the solution for most of the obstacles related to plant biomass harvesting for secondary metabolites production comes with in vitro bioprocessing of hairy roots [ 6 , 7 , 8 , 9 ]. Due to stable genetic alterations induced by Rhizobium rhizogenes , hairy root features intensified growth in a culture media free of plant growth regulators, with the propensity for lateral branching and enhanced biosynthesis of secondary metabolites specific for root tissue differentiation [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Selective Impact of MTMS-Based Xerogel Morphology on Boosted Proliferation and Enhanced Naphthoquinone Production in Cultures of Rindera graeca Transgenic Roots

Wierzchowski

Nowak

Kawka

et al. 2022

IJMS

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In situ extraction is a method for separating plant secondary metabolites from in vitro systems of plant biomass cultures. The study aimed to investigate the MTMS-based xerogels morphology effect on the growth kinetics and deoxyshikonin productivity in xerogel-supported in vitro culture systems of Rindera graeca hairy root. Cultures were supplemented with three types of xerogel, i.e., mesoporous gel, microporous gel, and agglomerated precipitate, in the disintegrated or monolithic form. Structure, oil sorption capacity, and SEM analyses for xerogel-based additives were performed. Application of monolithic macroporous xerogel resulted in the highest biomass proliferation, i.e., 5.11-fold fresh biomass increase after four weeks of the screening culture. The highest deoxyshikonin production (i.e., 105.03 µg) was noted when hairy roots were maintained with particles of disintegrated mesoporous xerogel. The detailed kinetics investigations (6-week culture) revealed the highest growth of hairy root biomass and secondary metabolite production, equaling 9.46-fold fresh weight biomass and 204.08 µg deoxyshikonin, respectively. MTMS-based xerogels have been recognized as selective biocompatible scaffolds for boosting the proliferation of transgenic roots or for productivity enhancement of naphthoquinones without detrimental effects on biomass growth, and their successful applicability in in situ removal of secondary plant metabolites has been experimentally confirmed.

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Hairy root culture technology: applications, constraints and prospect

Cited by 51 publications

References 198 publications

Advances in biotechnology of Emblica officinalis Gaertn. syn. Phyllanthus emblica L.: a nutraceuticals-rich fruit tree with multifaceted ethnomedicinal uses

Advances in biotechnology of Emblica officinalis Gaertn. syn. Phyllanthus emblica L.: a nutraceuticals-rich fruit tree with multifaceted ethnomedicinal uses

Hairy Root Cultures as a Source of Polyphenolic Antioxidants: Flavonoids, Stilbenoids and Hydrolyzable Tannins

Selective Impact of MTMS-Based Xerogel Morphology on Boosted Proliferation and Enhanced Naphthoquinone Production in Cultures of Rindera graeca Transgenic Roots

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