Hairy Root Cultures for Monoterpene Indole Alkaloid Pathway: Investigation and Biotechnological Production

Mehrotra, Shakti; Mishra, Sonal; Srivastava, Vikas

doi:10.1007/978-981-13-2562-5_5

Cited by 9 publications

(4 citation statements)

References 92 publications

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“…Later, genetically modified and/or transgenic hairy root cultures are developed, being a great achievement in the system ( Mehrotra et al., 2010 ). A number of research using genetic engineering technology have been realized to improve the production of different kinds of pharmaceutically valuable plant-based bioactive metabolites ( Grzegorczyk-Karolak et al., 2018 ; Mehrotra et al., 2018 ; Sahai and Sinha, 2022 ).…”

Section: Tissue Culture-based Biotechnological Approaches For Obtaini...mentioning

confidence: 99%

Production of secondary metabolites using tissue culture-based biotechnological applications

et al. 2023

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Plants are the sources of many bioactive secondary metabolites which are present in plant organs including leaves, stems, roots, and flowers. Although they provide advantages to the plants in many cases, they are not necessary for metabolisms related to growth, development, and reproduction. They are specific to plant species and are precursor substances, which can be modified for generations of various compounds in different plant species. Secondary metabolites are used in many industries, including dye, food processing and cosmetic industries, and in agricultural control as well as being used as pharmaceutical raw materials by humans. For this reason, the demand is high; therefore, they are needed to be obtained in large volumes and the large productions can be achieved using biotechnological methods in addition to production, being done with classical methods. For this, plant biotechnology can be put in action through using different methods. The most important of these methods include tissue culture and gene transfer. The genetically modified plants are agriculturally more productive and are commercially more effective and are valuable tools for industrial and medical purposes as well as being the sources of many secondary metabolites of therapeutic importance. With plant tissue culture applications, which are also the first step in obtaining transgenic plants with having desirable characteristics, it is possible to produce specific secondary metabolites in large-scale through using whole plants or using specific tissues of these plants in laboratory conditions. Currently, many studies are going on this subject, and some of them receiving attention are found to be taken place in plant biotechnology and having promising applications. In this work, particularly benefits of secondary metabolites, and their productions through tissue culture-based biotechnological applications are discussed using literature with presence of current studies.

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Section: Tissue Culture-based Biotechnological Approaches For Obtaini...mentioning

confidence: 99%

Production of secondary metabolites using tissue culture-based biotechnological applications

et al. 2023

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“…There are diverse alkaloids found in nature, such as steroidal, pyrrole, pyrrolidine, tropane and indole alkaloids. Indole alkaloids are the largest group of alkaloids found in plants with indole skeletons ( Hesse, 2002 ; Mehrotra et al., 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Hairy root culture: a potent method for improved secondary metabolite production of Solanaceous plants

Biswas,

Chakraborty,

Mukherjee

et al. 2023

Front. Plant Sci.

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Secondary metabolites synthesized by the Solanaceous plants are of major therapeutic and pharmaceutical importance, many of which are commonly obtained from the roots of these plants. ‘Hairy roots’, mirroring the same phytochemical pattern of the corresponding root of the parent plant with higher growth rate and productivity, are therefore extensively studied as an effective alternative for the in vitro production of these metabolites. Hairy roots are the transformed roots, generated from the infection site of the wounded plants with Agrobacterium rhizogenes. With their fast growth, being free from pathogen and herbicide contamination, genetic stability, and autotrophic nature for plant hormones, hairy roots are considered as useful bioproduction systems for specialized metabolites. Lately, several elicitation methods have been employed to enhance the accumulation of these compounds in the hairy root cultures for both small and large-scale production. Nevertheless, in the latter case, the cultivation of hairy roots in bioreactors should still be optimized. Hairy roots can also be utilized for metabolic engineering of the regulatory genes in the metabolic pathways leading to enhanced production of metabolites. The present study summarizes the updated and modern biotechnological aspects for enhanced production of secondary metabolites in the hairy root cultures of the plants of Solanaceae and their respective importance.

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“…To date, more than 3000 MIAs have been identified with diverse structures and bioactivities, which are exemplified by three of the most well-known members: quinine ( 1 ), strychnine ( 2 ), and vinblastine ( 3 ) (Figure ). − Quinine ( 1 ) belongs to the family of Cinchona alkaloids and is an antimalarial drug; − strychnine ( 2 ), one of the most complex Strychnos alkaloids, is a potent toxin; − and vinblastine ( 3 ), a Vinca alkaloid, is a frontline anticancer therapeutic and one of the most expensive small-molecule, off-patent drugs on the pharmaceutical market. − …”

Section: Introductionmentioning

confidence: 99%

Total Synthesis of (−)-Strictosidine and Interception of Aryne Natural Product Derivatives “Strictosidyne” and “Strictosamidyne”

et al. 2021

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Monoterpene indole alkaloids are a large class of natural products derived from a single biosynthetic precursor, strictosidine. We describe a synthetic approach to strictosidine that relies on a key facially selective Diels–Alder reaction between a glucosyl-modified alkene and an enal to set the C15–C20–C21 stereotriad. DFT calculations were used to examine the origin of stereoselectivity in this key step, wherein two of 16 possible isomers are predominantly formed. These calculations suggest the presence of a glucosyl unit, also inherent in the strictosidine structure, guides diastereoselectivity, with the reactive conformation of the vinyl glycoside dienophile being controlled by an exo-anomeric effect. (−)-Strictosidine was subsequently accessed using late-stage synthetic manipulations and an enzymatic Pictet–Spengler reaction. Several new natural product analogs were also accessed, including precursors to two unusual aryne natural product derivatives termed “strictosidyne” and “strictosamidyne”. These studies provide a strategy for accessing glycosylic natural products and a new platform to access monoterpene indole alkaloids and their derivatives.

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Hairy Root Cultures for Monoterpene Indole Alkaloid Pathway: Investigation and Biotechnological Production

Cited by 9 publications

References 92 publications

Production of secondary metabolites using tissue culture-based biotechnological applications

Production of secondary metabolites using tissue culture-based biotechnological applications

Hairy root culture: a potent method for improved secondary metabolite production of Solanaceous plants

Total Synthesis of (−)-Strictosidine and Interception of Aryne Natural Product Derivatives “Strictosidyne” and “Strictosamidyne”

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