Hairy roots production in Phyllanthus odontadenius Mll. Arg. by seedlings transformed with Agrobacterium rhizogenes A4RS/pHKN29

Abstract

Phyllanthus odontadenius Müll. Arg is one of the most important medicinal plants in the genusPhyllanthus (Phyllanthaceae). It is used in different regions in the world for the treatment of various diseases for example malaria caused by Plasmodium falciparum causing millions deaths in tropical and subtropical regions. In this work, we experimented P. odontadenius seeds germination and seedlings infected by Agrobacterium rhizogenes A4RS/pHKN9. We obtained 36% of seeds that germinated for 84.6% of seedlings transformed which showed positive gfp activities. The number of hairy roots formed in infectious sites for seedlings infected with A. rhizogenes A4RS/pHKN29 is valued at 6.3±2.71 against 0 for control seedlings. Diagrams showed that mean length for the new-formed roots were 1.92±0.55 cm and 1.59±0.49 cm for hairy root.

Acute Toxicity and In vivo Antioxidant Activity Evaluation of Aqueous Extracts from Phyllanthus odontadenius Müll. Arg.

“…odontadenius has been shown to have a strong antioxidant potential in the various studies carried out on the in vitro antioxidant activity of its various extracts used, thus corroborating its traditional uses [4,5]. The antioxidant potential of P. odontadenius would be explained in its composition in secondary metabolites previously analyzed by various authors and which was found to be rich in phenolic compounds [6,7,8]. Phytochemical and pharmacological studies carried out attest to success of P. odontadenius in therapy where the extracts of the twigs and leaves reveal antispasmodic, anticancer, antiplasmodial, antidysenteric, antifungal, analgesic properties, etc.…”