Halalkalibacterium roseum gen. nov., sp. nov., a new member of the family Balneolaceae isolated from soil

Abstract

A Gram-stain-negative, non-motile, moderately halophilic and facultatively anaerobic bacterium, designated YR4-1T, was isolated from a saline-alkali and sorghum-planting soil sample collected in Dongying, Shandong Province, PR China. Growth occurred at 28–45 °C with the presence of 4.0–20.0 % (w/v) NaCl and pH 6.0–9.0. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that YR4-1T shared the highest similarity of 92.1–92.4 % with the valid published species of Aliifodinibius . The isolate formed a separate clade at the genus level in recently described family Balneolaceae . The draft genome of strain YR4-1T is 3.83 Mbp long with 44.0 mol% G+C content. The strain possesses several genes involved in the osmotic stress response mechanism and diverse metabolic pathways, probably for the living in saline environment. This may lead to a better understanding of the underrepresented Balneolaceae lineage. The major menaquinone was MK-7. The main polar lipid profile was composed of diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipids, aminophosphoglycolipid, one glycolipid, and four unidentified lipids. The predominant cellular fatty acids were iso-C15 : 0 (35.7 %) and anteiso-C15 : 0 (33.5 %). On the basis of its phenotypic, chemotaxonomic and phylogenetic features, strain YR4-1T represents a novel species of a new genus, for which the name Halalkalibacterium roseum gen. nov., sp. nov. is proposed. The type strain is YR4-1T (=CGMCC 1.17777T=KCTC 72795T).

Vertical organization of microbial communities in Salineta hypersaline wetland, Spain

“…For the deeper layers, the biomarkers revealed by LEfSe analysis correspond to OTUs affiliated with halophilic bacteria found in hypersaline ecosystems. They include Coxiella, Anaerophaga, and Balneolaceae for layer L2, exhibiting anaerobic metabolism (Song et al, 2021;Wu et al, 2022). This finding is in agreement with the reversed redox gradient observed in the Salineta wetland.…”

Biotin pathway in novel Fodinibius salsisoli sp. nov., isolated from hypersaline soils and reclassification of the genus Aliifodinibius as Fodinibius

“…The family Balneolaceae ( Xia et al, 2016 ) belongs to the phylum Balneolota , class Balneolia ( Munoz et al, 2016 ), and includes six genera: Aliifodinibius , Balneola , Fodinibius , Gracilimonas , Halalkalibaculum , and Rhodohalobacter ( Parte et al, 2020 ), which are found in marine and hypersaline environments, such as deep-sea sediments, salt-mines, salterns, and saline soils ( Urios et al, 2006 ; Choi et al, 2009 ; Wang et al, 2012 , 2013 ; Cho et al, 2013 ; Xia et al, 2017 ; Wu et al, 2022a , b ). The genus Fodinibius was proposed in 2012 based on a single species, Fodinibius salinus ( Wang et al, 2012 ) and the genus Aliifodinibius was firstly described only one year later ( Wang et al, 2013 ), including at that time two species, Aliifodinibius roseus (type species) and Aliifodinibius sediminis .…”

Discovery of the Streamlined Haloarchaeon Halorutilus salinus , Comprising a New Order Widespread in Hypersaline Environments across the World

“…Although traditional cultivation techniques have led to the isolation and description of hundreds of new groups of halophilic bacteria and archaea ( 19 , 22 – 30 ), a great number of recent culture-independent studies from hypersaline habitats have revealed the prominence of several yet-uncultivated lineages in these systems ( 31 – 34 ). Most of these uncultivated groups represent dominant populations in hypersaline environments ( 31 , 32 , 35 , 36 ), and are presumably slow growing and fastidious to isolate microorganisms.…”