Halloysite and chitosan oligosaccharide nanocomposite for wound healing

Sandri, Giuseppina; Aguzzi, Carola; Rossi, Silvia; Bonferoni, Maria Cristina; Bruni, Giovanna; Boselli, Cinzia; Cornaglia, Antonia Icaro; Riva, Federica; Viseras, César; Caramella, Carla; Ferrari, Franca

doi:10.1016/j.actbio.2017.05.032

Cited by 147 publications

(57 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In vitro cytotoxicity tests toward Caco‐2 cell line results revealed that, except for samples 5 (100 μg/mL) and 6 (1000 μg/mL), all the HLNTs concentrations tested were biocompatible toward Caco‐2 cell lines (providing viability values not lower than the positive control). These results are in agreement with previously reported literature data . In addition, proliferative effects were found for concentrations 1–4, since the obtained viability values were significantly higher than the positive control.…”

Section: Discussionsupporting

confidence: 93%

“…These results are in agreement with previously reported literature data. [40][41][42] In addition, proliferative effects were found for concentrations 1-4, since the obtained viability values were significantly higher than the positive control. This effect was in line with previous works in which HLNTs were found to have a positive influx on cell attachment and spreading, even higher than other similar inorganic carriers due to the higher roughness of its surface in comparison with the smooth of silica or montmorillonite.…”

Section: Discussionmentioning

confidence: 94%

See 1 more Smart Citation

Halloysite nanotubes as tools to improve the actual challenge of fixed doses combinations in tuberculosis treatment

Carazo

Sandri

Cerezo

et al. 2019

J Biomedical Materials Res

Self Cite

View full text Add to dashboard Cite

Halloysite nanotubes (HLNTs) were used as nanocarriers of the tuberculostatic agent isoniazid (INH), a BCS (Biopharmaceutics Classification System) class III drug. Self‐assembling nanohybrids (INH‐loaded HLNTs) with an average outer diameter of 90 nm and polydispersity index of 0.7 approximately, were obtained by spontaneous adsorption of INH molecules to HLNTs powder in aqueous medium. The nanohybrids were aimed to improve oral drug bioavailability and reduce physicochemical incompatibility of INH with other concomitantly administered tuberculostatic agents. In vitro drug release from INH‐loaded HLNTs was successfully fitted to a diffusive kinetic law founded on the adsorption–desorption equilibrium between drug molecules in solution and solid inorganic excipients. INH‐loaded HLNTs showed good in vitro biocompatibility toward Caco‐2 cells at the concentrations studied (up to 1233 μg/mL), with improved cell proliferation. Permeability tests showed that INH transport across Caco‐2 cellular membranes was greatly enhanced and fluorescent microscopy confirmed that the drug encapsulated into nanohybrid was effectively internalized by the cells. INH‐loaded HLNTs enhanced stability of the drug in presence of other tuberculostatic agents, both in binary and quaternary combinations. It has been demonstrated that simple interaction between INH with HLNTs leads to drug permeability and stability improvements that could greatly facilitate the design of multiple drug dosage forms, an actual challenge in oral treatment of tuberculosis. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2019.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 94%

Halloysite nanotubes as tools to improve the actual challenge of fixed doses combinations in tuberculosis treatment

Carazo

Sandri

Cerezo

et al. 2019

J Biomedical Materials Res

Self Cite

View full text Add to dashboard Cite

show abstract

“…Recent studies on chitosan matrices reinforced with HNTs are mainly focused on their physicochemical and mechanical characterization, biocompatibility, and wound‐healing properties. Published results demonstrate improved potential of these composite matrices for tissue engineering and wound healing application, owing to cytocompatibility, improved skin reepithelialization and collagen deposition effects, mechanical strength and thermal stability, and reduced swelling ability in comparison to chitosan matrices. Furthermore, incorporation of HNTs and other clays, such as montmorillonite, into crosslinked chitosan particles retards drug release as a consequence of polymer network reinforcement via noncovalent interactions between chitosan and the clay mineral .…”

Section: Introductionmentioning

confidence: 92%

Functionality of chitosan‐halloysite nanocomposite films for sustained delivery of antibiotics: The effect of chitosan molar mass

Čalija

Milić

Milašinović

et al. 2019

J of Applied Polymer Sci

View full text Add to dashboard Cite

This study was designed to investigate functionality of tetracycline‐loaded chitosan‐halloysite nanocomposite films, with focus on evaluating the influence of chitosan molar mass on films applicability for sustained local antibiotic delivery. The films were prepared by casting and solvent evaporation using low, medium, and high molar mass chitosan. SEM analysis revealed compact, nonporous and rough surface of the nanocomposite films due to the presence of halloysite agglomerates and tetracycline crystals. Increasing chitosan molar mass led to higher values of elongation at break (from 21.65 ± 2.65 to 34.48 ± 2.34%), tensile strength (from 134.8 ± 13.21 to 246.36 ± 14.69 MPa), and elastic modulus (from 633.79 ± 128.37 to 716.55 ± 60.76 MPa) of the nanocomposite films. FT‐IR, XRPD, and thermal analyses confirmed molar mass dependent chitosan‐halloysite interactions and improved thermal stability of the nanocomposite films in comparison with chitosan films. The nanocomposite films released tetracycline in a sustained manner, with the slowest release achieved from the films consisting of low molar mass chitosan. Chitosan molar mass was confirmed to be a functionality‐related characteristic of chitosan‐halloysite nanocomposite films as potential sustained‐release carriers for topical delivery of antibiotics. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020, 137, 48406.

show abstract

“…In vivo case studies are still rather limited. Examples include (i) functionalised wound dressing materials like HTNs/chitosan 64 and HNTs/poly(L-lactide) 61 which demonstrated improved skin reepithelialization; (ii) nanocomposites for bone tissue regeneration 65 wherein HNTs themselves stimulated osteogenic differentiation of cells and improved bone repair; (iii) HNT-functionalised alloys with improved corrosion resistance for orthopaedic applications 66 and (iv) aligned composites with HNTs for guided nerve regeneration. 63…”

Section: Hnts For Tissue Engineeringmentioning

confidence: 99%

Naturally derived nano- and micro-drug delivery vehicles: halloysite, vaterite and nanocellulose

et al. 2020

View full text Add to dashboard Cite

show abstract

Halloysite and chitosan oligosaccharide nanocomposite for wound healing

Cited by 147 publications

References 22 publications

Halloysite nanotubes as tools to improve the actual challenge of fixed doses combinations in tuberculosis treatment

Halloysite nanotubes as tools to improve the actual challenge of fixed doses combinations in tuberculosis treatment

Functionality of chitosan‐halloysite nanocomposite films for sustained delivery of antibiotics: The effect of chitosan molar mass

Naturally derived nano- and micro-drug delivery vehicles: halloysite, vaterite and nanocellulose

Contact Info

Product

Resources

About