Halloysite nanotubes: a green resource for materials and life sciences

Massaro, Marina; Lazzara, Giuseppe; Noto, Renato; Riela, Serena

doi:10.1007/s12210-020-00886-x

Cited by 37 publications

(13 citation statements)

References 54 publications

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“…Indeed, it is known in literature that macrophages are able to degrade halloysite. 56 The green fluorescence signal can be clearly seen inside both cell lines ( Figure 5A and B ) by fluorescence microscopy. Green fluorescent signals inside the cells indicate that the compound can be internalized and that is localized in the cytoplasmic area, particularly in the perinuclear region.…”

Section: Resultsmentioning

confidence: 90%

Study of Uptake Mechanisms of Halloysite Nanotubes in Different Cell Lines

Biddeci¹,

Massaro²,

Riela³

et al. 2021

IJN

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Halloysite nanotubes (HNTs) are a natural aluminosilicate clay with a chemical formula of Al 2 Si 2 O 5 (OH) 4 ×nH 2 O and a hollow tubular structure. Due to their peculiar structure, HNTs can play an important role as a drug carrier system. Currently, the mechanism by which HNTs are internalized into living cells, and what is the transport pathway, is still unclear. Therefore, this study aimed at establishing the in vitro mechanism by which halloysite nanotubes could be internalized, using phagocytic and non-phagocytic cell lines as models. Methods: The HNT/CURBO hybrid system, where a fluorescent probe (CURBO) is confined in the HNT lumen, has been used as a model to study the transport pathway mechanisms of HNTs. The cytocompatibility of HNT/CURBO on cell lines model was investigated by MTS assay. In order to identify the internalization pathway involved in the cellular uptake, we performed various endocytosis-inhibiting studies, and we used fluorescence microscopy to verify the nanomaterial internalization by cells. We evaluated the haemolytic effect of HNT/CURBO placed in contact with human red blood cells (HRBCs), by reading the absorbance value of the supernatant at 570 nm. Results: The HNT/CURBO is highly biocompatible and does not have an appreciable haemolytic effect. The results of the inhibition tests have shown that the internalization process of nanotubes occurs in an energy-dependent manner in both the investigated cell lines, although they have different characteristics. In particular, in non-phagocytic cells, clathrin-dependent and independent endocytosis are involved. In phagocytic cells, in addition to phagocytosis and clathrin-dependent endocytosis, microtubules also participate in the halloysite cellular trafficking. Upon internalization by cells, HNT/CURBO is localized in the cytoplasmic area, particularly in the perinuclear region. Conclusion: Understanding the cellular transport pathways of HNTs can help in the rational design of novel drug delivery systems and can be of great value for their applications in biotechnology.

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Section: Resultsmentioning

confidence: 90%

Study of Uptake Mechanisms of Halloysite Nanotubes in Different Cell Lines

Biddeci¹,

Massaro²,

Riela³

et al. 2021

IJN

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“…In recent years, halloysite nanotubes have been widely researched as a "green resource" for their applications as drug carriers, for sustained drug release, and catalysis, to name a few [47,48]. Their function as an adsorptive material [49] is an application of great interest.…”

Section: Process Optimizationmentioning

confidence: 99%

Adsorption Method for the Remediation of Brilliant Green Dye Using Halloysite Nanotube: Isotherm, Kinetic and Modeling Studies

et al. 2021

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The first-ever use of halloysite nanotube (HNT), a relatively low-cost nanomaterial abundantly available with minor toxicity for removing brilliant green dye from aqueous media, is reported. The factors affecting adsorption were studied by assessing the adsorption capacity, kinetics, and equilibrium thermodynamic properties. All the experiments were designed at a pH level of around 7. The Redlich-Peterson isotherm model fits best amongst the nine isotherm models studied. The kinetic studies data confirmed a pseudo model of the second order. Robotic investigations propose a rate-controlling advance being overwhelmed by intraparticle dispersion. The adsorbent features were interpreted using infrared spectroscopy and electron microscopy. Process optimization was carried out using Response Surface Methodology (RSM) through a dual section Fractional Factorial Experimental Design to contemplate the impact of boundaries on the course of adsorption. The examination of fluctuation (ANOVA) was utilized to consider the joined impact of the boundaries. The possibilities of the use of dye adsorbing HNT (“sludge”) for the fabrication of the composites using plastic waste are suggested.

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“…Up to now different chemically and biologically active molecules with a negative electron density have been successfully loaded on HNTs including insulin [ 76 ], vancomycin [ 77 ], bovine serum albumin [ 78 ], isoniazid [ 78 ], carvacrol [ 79 ], etc. [ 80 , 81 , 82 ].…”

Section: Supramolecular Functionalizationmentioning

confidence: 99%

Past, Present and Future Perspectives on Halloysite Clay Minerals

2020

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Halloysite nanotubes (HNTs), clay minerals belonging to the kaolin groups, are emerging nanomaterials which have attracted the attention of the scientific community due to their interesting features, such as low-cost, availability and biocompatibility. In addition, their large surface area and tubular structure have led to HNTs’ application in different industrial purposes. This review reports a comprehensive overview of the historical background of HNT utilization in the last 20 years. In particular it will focus on the functionalization of the surfaces, both supramolecular and covalent, following applications in several fields, including biomedicine, environmental science and catalysis.

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Halloysite nanotubes: a green resource for materials and life sciences

Cited by 37 publications

References 54 publications

Study of Uptake Mechanisms of Halloysite Nanotubes in Different Cell Lines

Study of Uptake Mechanisms of Halloysite Nanotubes in Different Cell Lines

Adsorption Method for the Remediation of Brilliant Green Dye Using Halloysite Nanotube: Isotherm, Kinetic and Modeling Studies

Past, Present and Future Perspectives on Halloysite Clay Minerals

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