Haloalkaliphilic spore-forming sulfidogens from soda lake sediments and description of Desulfitispora alkaliphila gen. nov., sp. nov.

Sorokin, Dimitry Y.; Muyzer, Gerard

doi:10.1007/s00792-010-0310-y

Cited by 31 publications

(2 citation statements)

References 27 publications

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“…The sseA gene is actively transcribed on multiple contigs predominantly in the two deepest and most saline wells, CSWold (71.23 FPKM) and N08-B (188.53 FPKM), though it is ubiquitous at CROMO. Desulfitispora has also been shown to utilize thiosulfate, sulfite and elemental sulfur as electron acceptors (Sorokin and Muyzer, 2010) and may be involved with the cycling of these compounds.…”

Section: Microbial Cycling Of Intermediate Sulfur Compoundsmentioning

confidence: 99%

A dynamic microbial sulfur cycle in a serpentinizing continental ophiolite

Sabuda

Brazelton

Putman

et al. 2020

Environmental Microbiology

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Serpentinization is the hydration and oxidation of ultramafic rock, which occurs as oceanic lithosphere is emplaced onto continental margins (ophiolites), and along the seafloor as faulting exposes this mantle-derived material to circulating hydrothermal fluids. This process leads to distinctive fluid chemistries as molecular hydrogen (H 2 ) and hydroxyl ions (OH − ) are produced and reduced carbon compounds are mobilized. Serpentinizing ophiolites also serve as a vector to transport sulfur compounds from the seafloor onto the continents. We investigated hyperalkaline, sulfur-rich, brackish groundwater in a serpentinizing continental ophiolite to elucidate the role of sulfur compounds in fuelling in situ microbial activities. Here we illustrate that key sulfur-cycling taxa, including Dethiobacter, Desulfitispora and 'Desulforudis', persist throughout this extreme environment. Biologically catalysed redox reactions involving sulfate, sulfide and intermediate sulfur compounds are thermodynamically favourable in the groundwater, which indicates they may be vital to sustaining life in these characteristically oxidant-and energy-limited systems. Furthermore, metagenomic and metatranscriptomic analyses reveal a complex network involving sulfate reduction, sulfide oxidation and thiosulfate reactions. Our findings highlight the importance of the complete inorganic sulfur cycle in serpentinizing fluids and suggest sulfur biogeochemistry provides a key link between terrestrial serpentinizing ecosystems and their submarine heritage.

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Section: Microbial Cycling Of Intermediate Sulfur Compoundsmentioning

confidence: 99%

A dynamic microbial sulfur cycle in a serpentinizing continental ophiolite

Sabuda

Brazelton

Putman

et al. 2020

Environmental Microbiology

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“…So, it may be considered as a “crippled” SRB lacking a pathway for sulfate activation. Interestingly, when grown together, the growth rate and final biomass yield of the consortium with thiosulfate was higher, than for Desulfitispora alone, indicating a positive cooperation (Sorokin and Muyzer, 2010a ).…”

Section: Sulfidogenesis In Soda Lakesmentioning

confidence: 99%

The Microbial Sulfur Cycle at Extremely Haloalkaline Conditions of Soda Lakes

Sorokin¹,

Kuenen²,

Muyzer³

2011

Front. Microbio.

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169

120

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Soda lakes represent a unique ecosystem with extremely high pH (up to 11) and salinity (up to saturation) due to the presence of high concentrations of sodium carbonate in brines. Despite these double extreme conditions, most of the lakes are highly productive and contain a fully functional microbial system. The microbial sulfur cycle is among the most active in soda lakes. One of the explanations for that is high-energy efficiency of dissimilatory conversions of inorganic sulfur compounds, both oxidative and reductive, sufficient to cope with costly life at double extreme conditions. The oxidative part of the sulfur cycle is driven by chemolithoautotrophic haloalkaliphilic sulfur-oxidizing bacteria (SOB), which are unique for soda lakes. The haloalkaliphilic SOB are present in the surface sediment layer of various soda lakes at high numbers of up to 106 viable cells/cm3. The culturable forms are so far represented by four novel genera within the Gammaproteobacteria, including the genera Thioalkalivibrio, Thioalkalimicrobium, Thioalkalispira, and Thioalkalibacter. The latter two were only found occasionally and each includes a single species, while the former two are widely distributed in various soda lakes over the world. The genus Thioalkalivibrio is the most physiologically diverse and covers the whole spectrum of salt/pH conditions present in soda lakes. Most importantly, the dominant subgroup of this genus is able to grow in saturated soda brines containing 4 M total Na+ – a so far unique property for any known aerobic chemolithoautotroph. Furthermore, some species can use thiocyanate as a sole energy source and three out of nine species can grow anaerobically with nitrogen oxides as electron acceptor. The reductive part of the sulfur cycle is active in the anoxic layers of the sediments of soda lakes. The in situ measurements of sulfate reduction rates and laboratory experiments with sediment slurries using sulfate, thiosulfate, or elemental sulfur as electron acceptors demonstrated relatively high sulfate reduction rates only hampered by salt-saturated conditions. However, the highest rates of sulfidogenesis were observed not with sulfate, but with elemental sulfur followed by thiosulfate. Formate, but not hydrogen, was the most efficient electron donor with all three sulfur electron acceptors, while acetate was only utilized as an electron donor under sulfur-reducing conditions. The native sulfidogenic populations of soda lakes showed a typical obligately alkaliphilic pH response, which corresponded well to the in situ pH conditions. Microbiological analysis indicated a domination of three groups of haloalkaliphilic autotrophic sulfate-reducing bacteria belonging to the order Desulfovibrionales (genera Desulfonatronovibrio, Desulfonatronum, and Desulfonatronospira) with a clear tendency to grow by thiosulfate disproportionation in the absence of external electron donor even at salt-saturating conditions. Few novel representatives of the order Desulfobacterales capable of heterotrophic growth with volatile...

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Ecology and Biotechnology of Extremophilic Microorganisms, Particularly Anaerobic Thermophiles

Canganella¹

2012

Extremophiles

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Haloalkaliphilic spore-forming sulfidogens from soda lake sediments and description of Desulfitispora alkaliphila gen. nov., sp. nov.

Cited by 31 publications

References 27 publications

A dynamic microbial sulfur cycle in a serpentinizing continental ophiolite

A dynamic microbial sulfur cycle in a serpentinizing continental ophiolite

The Microbial Sulfur Cycle at Extremely Haloalkaline Conditions of Soda Lakes

Ecology and Biotechnology of Extremophilic Microorganisms, Particularly Anaerobic Thermophiles

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