Haloarcula hispanica spec. nov. and Haloferax gibbonsii spec, nov., Two New Species of Extremely Halophilic Archaebacteria

Juez, G.; Rodrı́guez-Valera, Francisco; Ventosa, António; Kushner, D. J.

doi:10.1016/s0723-2020(86)80152-7

Cited by 130 publications

(75 citation statements)

References 15 publications

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“…nov. is proposed. Mullakhanbhai & Larsen (1975), Rodriguez-Valera et al (1983), Tomlinson et al (1986), Juez et al (1986), Gutierrez et al (2002), Oren (2000) and Grant et al (2001), as well as from our comparative studies with H. volcanii DSM 33755 T . +, Positive; 2, negative; ND, not determined.…”

supporting

confidence: 71%

Haloferax sulfurifontis sp. nov., a halophilic archaeon isolated from a sulfide- and sulfur-rich spring

Elshahed

Savage

Oren

et al. 2004

International Journal of Systematic and Evolutionary Microbiology

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A pleomorphic, extremely halophilic archaeon (strain M6 T ) was isolated from a sulfide-and sulfur-rich spring in south-western Oklahoma (USA). It formed small (0?8-1?0 mm), salmon pink, elevated colonies on agar medium. The strain grew in a wide range of NaCl concentrations (6 % to saturation) and required at least 1 mM Mg 2+ for growth. Strain M6 T was able to reduce sulfur to sulfide anaerobically. 16S rRNA gene sequence analysis indicated that strain M6 T belongs to the family Halobacteriaceae, genus Haloferax; it showed 96?7-98?0 % similarity to other members of the genus with validly published names and 89 % similarity to Halogeometricum borinquense, its closest relative outside the genus Haloferax. Polar lipid analysis and DNA G+C content further supported placement of strain M6 T in the genus Haloferax. DNA-DNA hybridization values, as well as biochemical and physiological characterization, allowed strain M6 T to be differentiated from other members of the genus Haloferax. A novel species, Haloferax sulfurifontis sp. nov., is therefore proposed to accommodate the strain. The type strain is M6 T

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supporting

confidence: 71%

Haloferax sulfurifontis sp. nov., a halophilic archaeon isolated from a sulfide- and sulfur-rich spring

Elshahed

Savage

Oren

et al. 2004

International Journal of Systematic and Evolutionary Microbiology

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“…Alphasphaerolipoviruses display a narrow host range. Besides the common host, H. hispanica ATCC33960 [24], Halorubrum strain CSW 2.09.4 [42] was identified as an alternative host for SH1 and PH1, while SH1 and HHIV-2 could also infect Haloarcula sp. PV7 [5].…”

Section: Genus Alphasphaerolipovirusmentioning

confidence: 99%

Gammasphaerolipovirus, a newly proposed bacteriophage genus, unifies viruses of halophilic archaea and thermophilic bacteria within the novel family Sphaerolipoviridae

et al. 2014

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A new family of viruses named Sphaerolipoviridae has been proposed recently. It comprises icosahedral, tailless haloarchaeal viruses with an internal lipid membrane located between the protein capsid and the dsDNA genome. The proposed family Sphaerolipoviridae was divided into two genera: Alphasphaerolipovirus, including Haloarcula hispanica viruses SH1, PH1 and HHIV-2, and Betasphaerolipovirus, including Natrinema virus SNJ1. Here, we propose to expand the family Sphaerolipoviridae to include a group of bacteriophages infecting extreme thermophilic Thermus thermophilus and sharing a number of structural and genomic properties with archaeal sphaerolipoviruses. This new group comprises two members, lytic phage P23-77 and temperate phage IN93, as well as putative members P23-72 and P23-65H. In addition, several related proviruses have been discovered as integrated elements in bacterial genomes of the families Thermus and Meiothermus. Morphology of the virus particles and the overall capsid architecture of these bacteriophages resembles that of archaeal members of the Sphaerolipoviridae, including an unusual capsid arrangement in a T = 28 dextro lattice. Alpha-and betasphaerolipoviruses share with P23-77-like bacteriophages a conserved block of core genes that encode a putative genome-packaging ATPase and the two major capsid proteins (MCPs). The recently determined X-ray structure of the small and large MCPs of P23-77 revealed a single beta-barrel (jelly-roll) fold that is superimposable with the cryo-EM density maps of the SH1 capsomers. Given the common features of these viruses, we propose to include the so far unclassified P23-77-like bacteriophages into a new genus, ''Gammasphaerolipovirus'', within the family Sphaerolipoviridae.

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“…hispanica is capable to grow on acetate as a sole carbon source and possesses a gene cluster for the methylaspartate cycle enzymes, similar to H. marismortui (Figure 2; Juez et al, 1986;Liu et al, 2011b;Ding et al, 2014). In this study, we used deletion mutants of H. hispanica to prove the participation of the key enzymes of the methylaspartate cycle in acetate assimilation.…”

Section: Introductionmentioning

confidence: 99%

The methylaspartate cycle in haloarchaea and its possible role in carbon metabolism

et al. 2015

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Haloarchaea (class Halobacteria) live in extremely halophilic conditions and evolved many unique metabolic features, which help them to adapt to their environment. The methylaspartate cycle, an anaplerotic acetate assimilation pathway recently proposed for Haloarcula marismortui, is one of these special adaptations. In this cycle, acetyl-CoA is oxidized to glyoxylate via methylaspartate as a characteristic intermediate. The following glyoxylate condensation with another molecule of acetylCoA yields malate, a starting substrate for anabolism. The proposal of the functioning of the cycle was based mainly on in vitro data, leaving several open questions concerning the enzymology involved and the occurrence of the cycle in halophilic archaea. Using gene deletion mutants of H. hispanica, enzyme assays and metabolite analysis, we now close these gaps by unambiguous identification of the genes encoding all characteristic enzymes of the cycle. Based on these results, we were able to perform a solid study of the distribution of the methylaspartate cycle and the alternative acetate assimilation strategy, the glyoxylate cycle, among haloarchaea. We found that both of these cycles are evenly distributed in haloarchaea. Interestingly, 83% of the species using the methylaspartate cycle possess also the genes for polyhydroxyalkanoate biosynthesis, whereas only 34% of the species with the glyoxylate cycle are capable to synthesize this storage compound. This finding suggests that the methylaspartate cycle is shaped for polyhydroxyalkanoate utilization during carbon starvation, whereas the glyoxylate cycle is probably adapted for growth on substrates metabolized via acetyl-CoA.

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Haloarcula hispanica spec. nov. and Haloferax gibbonsii spec, nov., Two New Species of Extremely Halophilic Archaebacteria

Cited by 130 publications

References 15 publications

Haloferax sulfurifontis sp. nov., a halophilic archaeon isolated from a sulfide- and sulfur-rich spring

Haloferax sulfurifontis sp. nov., a halophilic archaeon isolated from a sulfide- and sulfur-rich spring

Gammasphaerolipovirus, a newly proposed bacteriophage genus, unifies viruses of halophilic archaea and thermophilic bacteria within the novel family Sphaerolipoviridae

The methylaspartate cycle in haloarchaea and its possible role in carbon metabolism

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