Halobacillus fulvus sp. nov., a moderately halophilic bacterium isolated from shrimp paste (Ka-pi) in Thailand

Abstract

An aerobic, Gram-stain-positive, endospore-forming, rod-shaped and moderately halophilic strain SKP4-6T, was isolated from shrimp paste (Ka-pi) collected from Samut Sakhon Province, Thailand. Phylogenetic analysis revealed that strain SKP4-6T belonged to the genus Halobacillus and was most closely related to Halobacillus salinus JCM 11546T (98.6 %), Halobacillus locisalis KCTC 3788T (98.6 %) and Halobacillus yeomjeoni KCTC 3957T (98.6 %) based on 16S rRNA gene sequence similarity. The digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strain SKP4-6T and its related species were 18.2–19.3 % and 69.84–84.51 %, respectively, which were lower than the threshold recommended for species delineation. The strain grew optimally at 30–40 °C, at pH 7.0 and with 10–15 % (w/v) NaCl. It contained l-Orn–d-Asp in the cell wall peptidoglycan. The DNA G+C content was 44.8 mol%. The major fatty acids were iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. The predominant isoprenoid quinone was MK-7. Phosphatidylglycerol and diphosphatidylglycerol were present as major polar lipids. Based on this polyphasic approach, digital DNA–DNA relatedness and ANI values, strain SKP4-6T represents a novel species of the genus Halobacillus , for which the name Halobacillus fulvus sp. nov. is proposed. The type strain is SKP4-6T (=JCM 32624T=TISTR 2595T).

Draft genome sequence of Halobacillus campisalis strain ASL-17

“…Spring et al ( 1 ) first described the genus Halobacillus, which belongs to the family Bacillaceae ( 2 , 3 ) within the phylum Firmicutes (synonym Bacillota , 3). Genus Halobacillus comprises 27 species: Halobacillus halophilus, Halobacillus litoralis , Halobacillus trueperi ( 1 ), Halobacillus thailandensis ( 4 ), Halobacillus salinus ( 5 ), Halobacillus karajensis ( 6 ) Halobacillus locisalis ( 7 ), Halobacillus styriensis ( 8 ), Halobacillus aidingensis and Halobacillus dabanensis ( 9 ), Halobacillus yeomjeoni ( 10 ), Halobacillus profundi and Halobacillus kuroshimensis ( 11 ), Halobacillus campisalis ( 12 ), Halobacillus faecis ( 13 ), Halobacillus mangrovi ( 14 ), Halobacillus alkaliphilus ( 15 ), Halobacillus seohaensis ( 16 ), Halobacillus naozhouensis and Halobacillus salsuginis ( 17 ), Halobacillus hunanensis ( 18 ), Halobacillus sediminis ( 19 ), Halobacillus andaensis ( 20 ), Halobacillus salicampi ( 21 ), Halobacillus massiliensis ( 22 ), Halobacillus marinus ( 23 ), Halobacillus ihumii ( 24 ), and Halobacillus fulvus ( 25 ). Members of the genus Halobacillus are moderate to highly halophilic ( 13 , 26 ), which makes them ideal candidates for biotechnological ( 27 ) as well as for astrobiological investigations ( 28 ).…”

Halobacillus salinarum sp. nov., Halobacillus shinanisalinarum sp. nov. and Halobacillus amylolyticus sp. nov., isolated from saltern soil

“…Due to salinity tolerance characteristics, Halobacillus species have been isolated from diverse saline environments such as solar salterns [3,5,[8][9][10][11], salt lakes [6,12,13], saline sediment and soils [1,14,15], and shrimp paste [2]. Solar salterns are extreme environments and known to harbour various halophilic microbes that are able to survive under saline conditions.…”

“…The genus Halobacillus belonging to the family Bacillaceae within the phylum Bacillota was proposed by Spring et al in 1996 [1]. At the time of writing, the genus contained 22 validly named species, along with the recently described Halobacillu fulvus [2] and Halobacillus salicampi [3], according to the List of Prokaryotic Names with Standing in Nomenclature [4]. Members of the genus are Gram-stain-positive, motile or non-motile, rod or cocci-shaped and endospore-forming at the central or subterminal positions.…”