Halofilum ochraceum gen. nov., sp. nov., a gammaproteobacterium isolated from a marine solar saltern

Abstract

A Gram-stain-negative, oxidase-negative, catalase-positive, facultative anaerobe, designated XJ16T, was isolated from a marine solar saltern on the coast of Weihai, China. Cells of strain XJ16T were long and rod-shaped. The colonies were ochre in colour and were able to reduce nitrate to nitrite. Optimal growth occurred at 33-37 °C (range, 20-45 °C) and in the presence of 8-10 % (w/v) NaCl (range, 2-20 %). The pH range for growth was found to be 6.5-9.5, with optimum growth at pH 7.5-8.0. Phylogenetic analysis based on the 16S rRNA gene sequence demonstrated that strain XJ16T was related to the phylum Proteobacteria. The most closely related neighbours were species of the genus Thioalkalivibrio, and the 16S rRNA gene sequence of strain XJ16T shared 93.1 % similarity with that of Thioalkalivibrio sulfidiphilus HL-EbGr7T and 93.0 % similarity with that of Thioalkalivibrio denitrificans ALJDT. The G+C content of the genomic DNA was 65.9 mol% (HPLC). The sole respiratory quinone was Q-8, and the predominant cellular fatty acids (>10 %) were iso-C15 : 0 2-OH/C16 : 1ω7c, C18 : 0 and C16 : 0 10-CH3. The predominant polar lipids in strain XJ16T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine. Based on these phylogenetic, physiological and biochemical characteristics, strain XJ16T should be classified representing a novel species of a new genus within the family Ectothiorhodospiraceae, for which the name Halofilum ochraceum gen. nov., sp. nov. is proposed. The type strain of the type species is XJ16T (=KCTC 42259T=MCCC 1H00120T=CICC 23817T).

Mining for Perchlorate Resistance Genes in Microorganisms From Sediments of a Hypersaline Pond in Atacama Desert, Chile

“…All predicted ORFs products were similar to proteins of different species of bacteria, mostly halophiles such as Halofilum ochraceum and Coraliomargarita sp. WN38, isolated from a marine solar saltern of Weihai (China) (Xia et al, 2017;Zhou et al, 2019), and Roseibaca ekhonensis, isolated from the hypersaline, heliothermal Ekho Lake (Antarctica) (Labrenz et al, 2009).…”

Dynamic of active microbial diversity in rhizosphere sediments of halophytes used for bioremediation of earthen shrimp ponds

“…These bacteria differ from oxygenic phototrophic bacteria by using sulphide, hydrogen or similar electron donors as the reducing power for photosynthesis rather than oxygen [ 70 ]. This function in the rhizosphere of Suaeda australis may be attributed to the evidence of biomarker Halofilum , purple sulphur bacteria, belonging to the Ectothiorhodospiraceae family [ 71 ]. In the context of sediment bioremediation, the hydrogen sulphide (H 2 S) oxidation by Ectothiorhodospiraceae family is of great interest because this compound is one of the biggest threats to aquaculture production due to its extreme toxicity to aquatic species such as shrimp [ 72 ].…”

Cross-kingdom metagenomic profiling of Lake Hillier reveals pigment-rich polyextremophiles and wide-ranging metabolic adaptations

“…Core species have been reported in different hosts (2; 36; 79) and environments (20; 22; 68) as functional contributors to the host survival and steady state of the environment. Most of Lake Hillier core species have been previously isolated from saline lakes in Senegal, Russia, Egypt, Spain (4; 63; 81; 83), solar salterns (19; 91; 96), contaminated environments (48; 50), and as algae symbionts (92). However, the taxonomic identity of half of the core species in Lake Hillier remains to be assigned.…”