Halofuginone triggers a transcriptional program centered on ribosome biogenesis and function in honey bees

Flores, Michelle Esthefani Retamozo; McNamara-Bordewick, Nora K.; Lovinger, Natalie L.; Snow, Jonathan W.

doi:10.1016/j.ibmb.2021.103667

Cited by 6 publications

(3 citation statements)

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“…To examine the expression of two genes that specifically mark translational stress in bees, we measured the expression of WD repeatcontaining protein 18 (Wd18) and WD repeat-containing protein 43 (Wd43). We have previously shown that these genes are transcriptionally upregulated after translation inhibition by either halofuginone or cycloheximide, but not other forms of stress (Figure 2A) [28]. Using qPCR, we found that the expression of WD43 and WD18 increased in the midguts of uninfected bees that were treated with paromomycin relative to the control bees after 4 days of feeding (Figure 4C).…”

Section: Paromomycin Impacts On the Expression Of General Stress Biom...mentioning

confidence: 69%

“…We found that both of these genes are induced after paromomycin treatment, suggesting that translation inhibition does occur. Although the cellular consequences of translation disruption in honey bees are not completely understood [18,28], a decrease in honey bee survival after paromomycin treatment is consistent with detrimental effects to the cell and tissue function. The increased expression of shsp genes also indicates that paromomycin has sublethal effects on honey bee midgut cells.…”

Section: Discussionmentioning

confidence: 96%

“…Conversely, in the fruit fly, pathogen-induced host translational blockages have been found to decrease both immune responses and epithelial renewal in the digestive tract [62]. We previously characterized the transcription response to translation inhibition in honey bee midgut tissue and identified a number of genes that are induced by translational stress (but not other types of stress), including Wd18 and Wd43 [28]. We found that both of these genes are induced after paromomycin treatment, suggesting that translation inhibition does occur.…”

Section: Discussionmentioning

confidence: 99%

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Paromomycin Reduces Vairimorpha (Nosema) ceranae Infection in Honey Bees but Perturbs Microbiome Levels and Midgut Cell Function

et al. 2022

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Paromomycin is a naturally occurring aminoglycoside antibiotic that has effects on both prokaryotic and eukaryotic microbes. However, previous reports have indicated that it has little effect on microsporidia, including Vairimorpha (Nosema) ceranae, in cell culture models. V. ceranae is one of a number of microsporidia species that cause disease in honey bees and substantial efforts to find new treatment strategies for bees that are infected with these pathogens are ongoing. When testing compounds for potential activity against V. ceranae in whole organisms, we found that paromomycin reduces the infection intensity of this parasite. Critically, the necessary doses of paromomycin have high activity against the bacteria of the honey bee microbiome and cause evident stress in bees. Microsporidia have been shown to lack an essential binding site on the ribosome that is known to allow for maximal inhibition by paromomycin. Thus, it is possible that paromomycin impacts parasite levels through non-cell autonomous effects on microsporidia infection levels via effects on the microbiome or midgut cellular function. As paromomycin treatment could cause widespread honey bee health issues in agricultural settings, it does not represent an appropriate anti-microsporidia agent for use in the field.

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Section: Paromomycin Impacts On the Expression Of General Stress Biom...mentioning

confidence: 69%

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

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Paromomycin Reduces Vairimorpha (Nosema) ceranae Infection in Honey Bees but Perturbs Microbiome Levels and Midgut Cell Function

et al. 2022

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What Does Cell Biology Have to Do with Saving Pollinators?

Snow

2023

Environmental Discourses in Science Education

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As a cell and molecular biologist trained in biomedical sciences, my early research focused on human disease etiology. I was fortunate to be able to realign my research and personal interests in sustainability when I began working on honey bee health as I transitioned to faculty positions at liberal arts colleges. As an Associate Professor of Biology at Barnard College, my teaching focuses on biology at the cell and molecular level, which is strongly associated with problems of human health. A major challenge I have faced centers around connecting the techniques and ways of thinking used in the study of cell biology with the broader environmental issues of honey bee disease and the growing pollinator crisis. In this chapter, I describe how I have worked to incorporate sustainability in a broad sense and justice in a somewhat narrow ecological sense into my lab and my teaching, with a specific focus on my course, Laboratory in Cell Biology. The lab class structure pursued here, which shares many aspects with the course-based undergraduate research experiences (CURE) model, seeks to create an authentic and inclusive environment to increase laboratory science involvement and enthusiasm in students from diverse backgrounds.

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Food contamination with fipronil alters gene expression associated with foraging in Africanized honey bees

Lima,

de Castro Lippi,

da Luz Scheffer

et al. 2024

Environ Sci Pollut Res

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Halofuginone triggers a transcriptional program centered on ribosome biogenesis and function in honey bees

Cited by 6 publications

References 81 publications

Paromomycin Reduces Vairimorpha (Nosema) ceranae Infection in Honey Bees but Perturbs Microbiome Levels and Midgut Cell Function

Paromomycin Reduces Vairimorpha (Nosema) ceranae Infection in Honey Bees but Perturbs Microbiome Levels and Midgut Cell Function

What Does Cell Biology Have to Do with Saving Pollinators?

Food contamination with fipronil alters gene expression associated with foraging in Africanized honey bees

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