Halomonas Jincaotanensis sp. nov., Isolated From the Pamir Plateau Degrading Polycyclic Aromatic Hydrocarbon

Abstract

A Gram-strain-negative, rod-shaped, aerobic bacterium, designated strain TRM 85114T, was isolated from the Jincaotan wetland in the Pamir Plateau of China. We identified this isolate was a novel species with an ability to degrade 1-naphthylamine. Strain grew optimally at 30 ℃ and pH 6.0 in the presence of 3% (w/v) NaCl. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain TRM 85114T was affiliated with the genus Halomonas, and shared high sequence similarity (97.3%) with Halomonas korlensis XK1T. Strain TRM 85114T contained C16:0 and C19:0 cyclo ω8c as primary cellular fatty acids, Q-9 as predominate respiratory quinone, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phospholipids of unknown structure containing glucosamine, unidentified aminophospholipids, unidentified lipids and three unidentified phospholipids as the major polar lipids. The complete genome of TRM 85114T comprises 3,902 putative genes with a total of 4,126,476 bp and a G + C content of 61.6 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between strain TRM 85114T and related type Halomonas strains was 78.6–88.9% and 25.7–39.2%, respectively. Based on phenotypic, chemotaxonomic, and molecular features, strain TRM 85114T represents a novel species of the genus Halomonas, duo to which the name Halomonas jincaotanensis sp. nov. is proposed. The type strain is TRM 85114T (CCTCC AB 2021006T = LMG 32311T). Degradation rate of 1-naphthylamine by strain TRM 85114T reached up to 32.0 mg/L in 14 days.